Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia
Objective: Indonesia is an archipelagic country with a mega biodiversity, among others, in the marine area. Seahorses (Hippocampus spp.) are a marine fish known to have biocompounds used in traditional medicine "Jamu," such as Hippocampus comes L. (HCL). The present study aims to a...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2022-12-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=90035 |
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| author | Trisnawati Mundijo Franciscus Dhyanagiri Suyatna Agung Eru Wibowo Agus Supriyono Yurnadi Hanafi Midoen |
| author_facet | Trisnawati Mundijo Franciscus Dhyanagiri Suyatna Agung Eru Wibowo Agus Supriyono Yurnadi Hanafi Midoen |
| author_sort | Trisnawati Mundijo |
| collection | DOAJ |
| description | Objective: Indonesia is an archipelagic country with a mega biodiversity, among others, in the marine area. Seahorses (Hippocampus spp.) are a marine fish known to have biocompounds used in traditional medicine "Jamu," such as Hippocampus comes L. (HCL). The present study aims to analyze and compare the chemical contents of cultured seahorse (CS) and natural seahorse (NS) extracts.
Materials and Methods: The CS and NS were identified morphometrically. After freeze-drying, the seahorses were ground into powder with a grinder. The seahorse powder was extracted with ethanol and a water solvent. The extract contained biocompounds, proximate, amino acids, and steroids with high-performance liquid chromatography.
Results: The study found unique characteristics of HCL. The highest yield was obtained in NS using a water solvent (18.6%). The biocompounds in seahorses consist of alkaloids and triterpenoids. The highest proximate of water content (11.03%) and ash content (42.50%) was found in NS. In addition, other compounds were also detected in CS, such as fat (7.48%) and protein (47.67%). Both of HCL's different sources had all essential and nonessential amino acids in which the highest concentration were in NS, i.e., L-arginine (56,537.22 mg/kg), L-lysine (17,794.17), glycine (113,649.80 mg/kg), L-proline (47,056.15), and L-alanine (43,451.81). The analysis of the steroid compound of the extract suggested the presence of steroid glycosides.
Conclusion: The highest yield of the seahorse extract with a water solvent is about 18.6% and protein content of 47.67% in CS. The crude extract has alkaloids, triterpenoids, and glycine (113,649.80 mg/kg) in NS with water, suggesting the presence of steroid glycosides. [J Adv Vet Anim Res 2022; 9(4.000): 610-616] |
| first_indexed | 2024-04-11T00:01:33Z |
| format | Article |
| id | doaj.art-caa64a1adece4beaacbd3875c60c26c3 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-04-11T00:01:33Z |
| publishDate | 2022-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-caa64a1adece4beaacbd3875c60c26c32023-01-10T04:13:29ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102022-12-019461061610.5455/javar.2022.i63090035Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, IndonesiaTrisnawati Mundijo0Franciscus Dhyanagiri Suyatna1Agung Eru Wibowo2Agus Supriyono3Yurnadi Hanafi Midoen4Doctoral Programme Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. & Department of Medical Biology, Faculty of Medicine, Universitas Muhammadiyah Palembang, Palembang, Indonesia Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Tangerang, Indonesia Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Tangerang, Indonesia Department of Medical Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.Objective: Indonesia is an archipelagic country with a mega biodiversity, among others, in the marine area. Seahorses (Hippocampus spp.) are a marine fish known to have biocompounds used in traditional medicine "Jamu," such as Hippocampus comes L. (HCL). The present study aims to analyze and compare the chemical contents of cultured seahorse (CS) and natural seahorse (NS) extracts. Materials and Methods: The CS and NS were identified morphometrically. After freeze-drying, the seahorses were ground into powder with a grinder. The seahorse powder was extracted with ethanol and a water solvent. The extract contained biocompounds, proximate, amino acids, and steroids with high-performance liquid chromatography. Results: The study found unique characteristics of HCL. The highest yield was obtained in NS using a water solvent (18.6%). The biocompounds in seahorses consist of alkaloids and triterpenoids. The highest proximate of water content (11.03%) and ash content (42.50%) was found in NS. In addition, other compounds were also detected in CS, such as fat (7.48%) and protein (47.67%). Both of HCL's different sources had all essential and nonessential amino acids in which the highest concentration were in NS, i.e., L-arginine (56,537.22 mg/kg), L-lysine (17,794.17), glycine (113,649.80 mg/kg), L-proline (47,056.15), and L-alanine (43,451.81). The analysis of the steroid compound of the extract suggested the presence of steroid glycosides. Conclusion: The highest yield of the seahorse extract with a water solvent is about 18.6% and protein content of 47.67% in CS. The crude extract has alkaloids, triterpenoids, and glycine (113,649.80 mg/kg) in NS with water, suggesting the presence of steroid glycosides. [J Adv Vet Anim Res 2022; 9(4.000): 610-616]http://www.ejmanager.com/fulltextpdf.php?mno=90035hippocampus comes l.; amino acid; steroid; cultured; nature |
| spellingShingle | Trisnawati Mundijo Franciscus Dhyanagiri Suyatna Agung Eru Wibowo Agus Supriyono Yurnadi Hanafi Midoen Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia Journal of Advanced Veterinary and Animal Research hippocampus comes l.; amino acid; steroid; cultured; nature |
| title | Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia |
| title_full | Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia |
| title_fullStr | Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia |
| title_full_unstemmed | Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia |
| title_short | Characterization of seahorse (Hippocampus comes L.) extracts originating from culture and nature in Pesawaran, Lampung, Indonesia |
| title_sort | characterization of seahorse hippocampus comes l extracts originating from culture and nature in pesawaran lampung indonesia |
| topic | hippocampus comes l.; amino acid; steroid; cultured; nature |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=90035 |
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