SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice
Skin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, ex...
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MDPI AG
2018-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/10/2611 |
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| author | Xionglin Chen Xiaoming Cao He Jiang Xiangxin Che Xiaoyuan Xu Baicheng Ma Jie Zhang Tao Huang |
| author_facet | Xionglin Chen Xiaoming Cao He Jiang Xiangxin Che Xiaoyuan Xu Baicheng Ma Jie Zhang Tao Huang |
| author_sort | Xionglin Chen |
| collection | DOAJ |
| description | Skin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment strategies that enhance angiogenesis and growth factors secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H&E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that seen in the negative and positive controls. Masson’s trichrome staining showed that more collagen fibers were deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. Immunohistochemistry assays demonstrated that more myofibroblasts were deposited and more angiogenesis occurred in skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. In addition, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of growth factors in skin wounds. Taken together, these results suggest that the SIKVAV-modified chitosan hydrogel has the potential to be developed as synthesized biomaterials for the treatment of skin wounds. |
| first_indexed | 2024-12-11T03:11:35Z |
| format | Article |
| id | doaj.art-caa6623600034f02995896e6e13ce27c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T03:11:35Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-caa6623600034f02995896e6e13ce27c2022-12-22T01:22:51ZengMDPI AGMolecules1420-30492018-10-012310261110.3390/molecules23102611molecules23102611SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in MiceXionglin Chen0Xiaoming Cao1He Jiang2Xiangxin Che3Xiaoyuan Xu4Baicheng Ma5Jie Zhang6Tao Huang7Department of Histology & Embryology and Medical Genetics, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Anatomy, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Histology & Embryology and Medical Genetics, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Anatomy, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Histology & Embryology and Medical Genetics, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Histology & Embryology and Medical Genetics, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Histology & Embryology and Medical Genetics, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaDepartment of Histology & Embryology and Medical Genetics, School of Basic Medical Sciences, Jiujiang University, Jiujiang 332000, ChinaSkin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment strategies that enhance angiogenesis and growth factors secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H&E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that seen in the negative and positive controls. Masson’s trichrome staining showed that more collagen fibers were deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. Immunohistochemistry assays demonstrated that more myofibroblasts were deposited and more angiogenesis occurred in skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. In addition, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of growth factors in skin wounds. Taken together, these results suggest that the SIKVAV-modified chitosan hydrogel has the potential to be developed as synthesized biomaterials for the treatment of skin wounds.http://www.mdpi.com/1420-3049/23/10/2611SIKVAVchitosan hydrogelskin wound healingangiogenesisgrowth factor |
| spellingShingle | Xionglin Chen Xiaoming Cao He Jiang Xiangxin Che Xiaoyuan Xu Baicheng Ma Jie Zhang Tao Huang SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice Molecules SIKVAV chitosan hydrogel skin wound healing angiogenesis growth factor |
| title | SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice |
| title_full | SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice |
| title_fullStr | SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice |
| title_full_unstemmed | SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice |
| title_short | SIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in Mice |
| title_sort | sikvav modified chitosan hydrogel as a skin substitutes for wound closure in mice |
| topic | SIKVAV chitosan hydrogel skin wound healing angiogenesis growth factor |
| url | http://www.mdpi.com/1420-3049/23/10/2611 |
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