Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin
Background: Emerging evidence has supported that intensive exercise induces weakened performance and immune and metabolic disorders. We systematically evaluated the effects of quercetin against hepatic inflammatory damage caused by repeated intensive exercise and explored the potential mechanism. Me...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2072-6643/12/9/2770 |
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| author | Chao Gao Yang Liu Chunjie Jiang Liang Liu Juan Li Dan Li Xiaoping Guo Zhu Wang Yuexin Yang Liegang Liu Ping Yao Yuhan Tang |
| author_facet | Chao Gao Yang Liu Chunjie Jiang Liang Liu Juan Li Dan Li Xiaoping Guo Zhu Wang Yuexin Yang Liegang Liu Ping Yao Yuhan Tang |
| author_sort | Chao Gao |
| collection | DOAJ |
| description | Background: Emerging evidence has supported that intensive exercise induces weakened performance and immune and metabolic disorders. We systematically evaluated the effects of quercetin against hepatic inflammatory damage caused by repeated intensive exercise and explored the potential mechanism. Methods: Male BALB/c mice were administered quercetin (100 mg/kg BW) for four weeks, and performed a treadmill running protocol of 28 m/min, 5° slope, 90 min/day concurrently for the last seven days. Results: Quercetin administration reduced the leakage of aspartic acid and alanine aminotransferase and improved ultrastructural abnormalities such as swelling, and degeneration caused by high-intensity running in mice. Quercetin significantly decreased the hepatic and plasmatic levels of inflammatory cytokines IL-1β, IL-6, TNF-α, inducible nitric oxide synthase, cyclooxygenase-2 and intercellular adhesion molecule-1—provoked by over-exercise. Furthermore, diminished activation and nuclear translocation of NF-κB were found after quercetin treatment through inhibiting IKKα and Iκbα phosphorylation of intensive running mice. Conclusion: Quercetin offers protection for mouse livers against intensive sports-induced inflammatory injury, and the suppression of the NF-κB signal transduction pathway may play a role in its anti-inflammatory effects. Our findings broaden our understanding of natural phytochemicals as a promising strategy to prevent excessive exercise damage. |
| first_indexed | 2024-03-10T16:24:51Z |
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| id | doaj.art-caac39b672214b028ebd5c1aa2008576 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T16:24:51Z |
| publishDate | 2020-09-01 |
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| series | Nutrients |
| spelling | doaj.art-caac39b672214b028ebd5c1aa20085762023-11-20T13:21:26ZengMDPI AGNutrients2072-66432020-09-01129277010.3390/nu12092770Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of QuercetinChao Gao0Yang Liu1Chunjie Jiang2Liang Liu3Juan Li4Dan Li5Xiaoping Guo6Zhu Wang7Yuexin Yang8Liegang Liu9Ping Yao10Yuhan Tang11Key Laboratory of Trace Element Nutrition of National Health Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, ChinaKey Laboratory of Trace Element Nutrition of National Health Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaKey Laboratory of Trace Element Nutrition of National Health Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, ChinaKey Laboratory of Trace Element Nutrition of National Health Commission, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaBackground: Emerging evidence has supported that intensive exercise induces weakened performance and immune and metabolic disorders. We systematically evaluated the effects of quercetin against hepatic inflammatory damage caused by repeated intensive exercise and explored the potential mechanism. Methods: Male BALB/c mice were administered quercetin (100 mg/kg BW) for four weeks, and performed a treadmill running protocol of 28 m/min, 5° slope, 90 min/day concurrently for the last seven days. Results: Quercetin administration reduced the leakage of aspartic acid and alanine aminotransferase and improved ultrastructural abnormalities such as swelling, and degeneration caused by high-intensity running in mice. Quercetin significantly decreased the hepatic and plasmatic levels of inflammatory cytokines IL-1β, IL-6, TNF-α, inducible nitric oxide synthase, cyclooxygenase-2 and intercellular adhesion molecule-1—provoked by over-exercise. Furthermore, diminished activation and nuclear translocation of NF-κB were found after quercetin treatment through inhibiting IKKα and Iκbα phosphorylation of intensive running mice. Conclusion: Quercetin offers protection for mouse livers against intensive sports-induced inflammatory injury, and the suppression of the NF-κB signal transduction pathway may play a role in its anti-inflammatory effects. Our findings broaden our understanding of natural phytochemicals as a promising strategy to prevent excessive exercise damage.https://www.mdpi.com/2072-6643/12/9/2770quercetinintensive exerciseinflammationliver damage |
| spellingShingle | Chao Gao Yang Liu Chunjie Jiang Liang Liu Juan Li Dan Li Xiaoping Guo Zhu Wang Yuexin Yang Liegang Liu Ping Yao Yuhan Tang Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin Nutrients quercetin intensive exercise inflammation liver damage |
| title | Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin |
| title_full | Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin |
| title_fullStr | Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin |
| title_full_unstemmed | Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin |
| title_short | Intensive Running Enhances NF-κB Activity in the Mice Liver and the Intervention Effects of Quercetin |
| title_sort | intensive running enhances nf κb activity in the mice liver and the intervention effects of quercetin |
| topic | quercetin intensive exercise inflammation liver damage |
| url | https://www.mdpi.com/2072-6643/12/9/2770 |
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