Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy
Abstract Background Allelopathy is closely associated with rhizosphere biological processes, and rhizosphere microbial communities are essential for plant development. However, our understanding of rhizobacterial communities under influence of allelochemicals in licorice remains limited. In the pres...
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BMC
2023-05-01
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| Series: | Microbiome |
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| Online Access: | https://doi.org/10.1186/s40168-023-01511-3 |
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| author | Yang Liu Hao Wang Xun Qian Jie Gu Weimin Chen Xihui Shen Shiheng Tao Shuo Jiao Gehong Wei |
| author_facet | Yang Liu Hao Wang Xun Qian Jie Gu Weimin Chen Xihui Shen Shiheng Tao Shuo Jiao Gehong Wei |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Abstract Background Allelopathy is closely associated with rhizosphere biological processes, and rhizosphere microbial communities are essential for plant development. However, our understanding of rhizobacterial communities under influence of allelochemicals in licorice remains limited. In the present study, the responses and effects of rhizobacterial communities on licorice allelopathy were investigated using a combination of multi-omics sequencing and pot experiments, under allelochemical addition and rhizobacterial inoculation treatments. Results Here, we demonstrated that exogenous glycyrrhizin inhibits licorice development, and reshapes and enriches specific rhizobacteria and corresponding functions related to glycyrrhizin degradation. Moreover, the Novosphingobium genus accounted for a relatively high proportion of the enriched taxa and appeared in metagenomic assembly genomes. We further characterized the different capacities of single and synthetic inoculants to degrade glycyrrhizin and elucidated their distinct potency for alleviating licorice allelopathy. Notably, the single replenished N (Novosphingobium resinovorum) inoculant had the greatest allelopathy alleviation effects in licorice seedlings. Conclusions Altogether, the findings highlight that exogenous glycyrrhizin simulates the allelopathic autotoxicity effects of licorice, and indigenous single rhizobacteria had greater effects than synthetic inoculants in protecting licorice growth from allelopathy. The results of the present study enhance our understanding of rhizobacterial community dynamics during licorice allelopathy, with potential implications for resolving continuous cropping obstacle in medicinal plant agriculture using rhizobacterial biofertilizers. Video Abstract |
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| last_indexed | 2024-03-13T09:00:48Z |
| publishDate | 2023-05-01 |
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| series | Microbiome |
| spelling | doaj.art-cab6df39a7df43878fa8f2fdd8dded5e2023-05-28T11:22:49ZengBMCMicrobiome2049-26182023-05-0111111810.1186/s40168-023-01511-3Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathyYang Liu0Hao Wang1Xun Qian2Jie Gu3Weimin Chen4Xihui Shen5Shiheng Tao6Shuo Jiao7Gehong Wei8State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityInterdisciplinary Research Center for Soil Microbial Ecology and Land Sustainable Productivity in Dry Areas, Northwest A&F UniversityInterdisciplinary Research Center for Soil Microbial Ecology and Land Sustainable Productivity in Dry Areas, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Science, Northwest A&F UniversityAbstract Background Allelopathy is closely associated with rhizosphere biological processes, and rhizosphere microbial communities are essential for plant development. However, our understanding of rhizobacterial communities under influence of allelochemicals in licorice remains limited. In the present study, the responses and effects of rhizobacterial communities on licorice allelopathy were investigated using a combination of multi-omics sequencing and pot experiments, under allelochemical addition and rhizobacterial inoculation treatments. Results Here, we demonstrated that exogenous glycyrrhizin inhibits licorice development, and reshapes and enriches specific rhizobacteria and corresponding functions related to glycyrrhizin degradation. Moreover, the Novosphingobium genus accounted for a relatively high proportion of the enriched taxa and appeared in metagenomic assembly genomes. We further characterized the different capacities of single and synthetic inoculants to degrade glycyrrhizin and elucidated their distinct potency for alleviating licorice allelopathy. Notably, the single replenished N (Novosphingobium resinovorum) inoculant had the greatest allelopathy alleviation effects in licorice seedlings. Conclusions Altogether, the findings highlight that exogenous glycyrrhizin simulates the allelopathic autotoxicity effects of licorice, and indigenous single rhizobacteria had greater effects than synthetic inoculants in protecting licorice growth from allelopathy. The results of the present study enhance our understanding of rhizobacterial community dynamics during licorice allelopathy, with potential implications for resolving continuous cropping obstacle in medicinal plant agriculture using rhizobacterial biofertilizers. Video Abstracthttps://doi.org/10.1186/s40168-023-01511-3AutotoxicityAllelochemicalRhizosphereContinuous cropping obstacleGlycyrrhiza uralensis Fisch. |
| spellingShingle | Yang Liu Hao Wang Xun Qian Jie Gu Weimin Chen Xihui Shen Shiheng Tao Shuo Jiao Gehong Wei Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy Microbiome Autotoxicity Allelochemical Rhizosphere Continuous cropping obstacle Glycyrrhiza uralensis Fisch. |
| title | Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy |
| title_full | Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy |
| title_fullStr | Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy |
| title_full_unstemmed | Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy |
| title_short | Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy |
| title_sort | metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy |
| topic | Autotoxicity Allelochemical Rhizosphere Continuous cropping obstacle Glycyrrhiza uralensis Fisch. |
| url | https://doi.org/10.1186/s40168-023-01511-3 |
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