Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity
Oxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content...
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| Format: | Article |
| Language: | English |
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Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2022-12-01
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| Series: | Jurnal Kimia Sains dan Aplikasi |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/52516 |
| _version_ | 1797862274124218368 |
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| author | Galuh Dwi Arum Mukhammad Asy'ari Nies Suci Mulyani |
| author_facet | Galuh Dwi Arum Mukhammad Asy'ari Nies Suci Mulyani |
| author_sort | Galuh Dwi Arum |
| collection | DOAJ |
| description | Oxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content of 2% to 30%. These bacteria can produce various kinds of pigments for self-defense from extreme environments, which function as immunomodulatory compounds and have antioxidant activity. The antioxidant activity of the yellow pigment halophilic bacteria Bacillus clausii J1G-0%B using the DPPH method showed that the effect of light and air on antioxidant activity was 17.88% inhibition in the crude extract and 14.24% inhibition at 1600 ppm. Antioxidant activity decreased by 17.13% under the influence of air, 1.89% under the influence of light, and 28.58% under the influence of air and light. FTIR analysis under the influence of air experienced a decrease in the peak of the CO carbonyl group of 1739.29 cm-1 and an increase in the alkane C-H group of 1369.52 cm-1, while under the influence of light and open conditions, the carbonyl CO group experienced an increase of 1739.50 cm-1 and 1739.46 cm-1 and the increase in alkane C-H groups 1371.41 cm-1 and 1369.53 cm- 1. |
| first_indexed | 2024-04-09T22:16:35Z |
| format | Article |
| id | doaj.art-cab7a30bf0314aca8afc56435da6bfea |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-04-09T22:16:35Z |
| publishDate | 2022-12-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-cab7a30bf0314aca8afc56435da6bfea2023-03-23T03:35:06ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142022-12-01251139940410.14710/jksa.25.11.399-40421724Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant ActivityGaluh Dwi Arum0Mukhammad Asy'ari1https://orcid.org/0000-0002-3489-1644Nies Suci Mulyani2Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, IndonesiaChemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, IndonesiaChemistry Department, Faculty of Sciences and Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang, IndonesiaOxidative stress is a state of excess free radicals in the body, which results in increased oxidation processes in the body’s cells and causes damage. In previous studies, one way to neutralize this situation was found, namely with halophilic bacteria. Halophilic bacteria can live at a salt content of 2% to 30%. These bacteria can produce various kinds of pigments for self-defense from extreme environments, which function as immunomodulatory compounds and have antioxidant activity. The antioxidant activity of the yellow pigment halophilic bacteria Bacillus clausii J1G-0%B using the DPPH method showed that the effect of light and air on antioxidant activity was 17.88% inhibition in the crude extract and 14.24% inhibition at 1600 ppm. Antioxidant activity decreased by 17.13% under the influence of air, 1.89% under the influence of light, and 28.58% under the influence of air and light. FTIR analysis under the influence of air experienced a decrease in the peak of the CO carbonyl group of 1739.29 cm-1 and an increase in the alkane C-H group of 1369.52 cm-1, while under the influence of light and open conditions, the carbonyl CO group experienced an increase of 1739.50 cm-1 and 1739.46 cm-1 and the increase in alkane C-H groups 1371.41 cm-1 and 1369.53 cm- 1.https://ejournal.undip.ac.id/index.php/ksa/article/view/52516halophilicbacillus clausiiantioxidantpigment |
| spellingShingle | Galuh Dwi Arum Mukhammad Asy'ari Nies Suci Mulyani Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity Jurnal Kimia Sains dan Aplikasi halophilic bacillus clausii antioxidant pigment |
| title | Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity |
| title_full | Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity |
| title_fullStr | Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity |
| title_full_unstemmed | Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity |
| title_short | Effect of Storage of Yellow Pigment from Halophilic Bacillus clausii J1G-0%B on Antioxidant Activity |
| title_sort | effect of storage of yellow pigment from halophilic bacillus clausii j1g 0 b on antioxidant activity |
| topic | halophilic bacillus clausii antioxidant pigment |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/52516 |
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