Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes
This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarc...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2016-07-01
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| Series: | Bioscience Journal |
| Subjects: | |
| Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32935 |
| _version_ | 1819289653214183424 |
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| author | Arthur Filipe Sousa Gomes Bruna Silveira Lamanes dos Santos Emanuele Giuliani Franciscon Milla Alves Baffi |
| author_facet | Arthur Filipe Sousa Gomes Bruna Silveira Lamanes dos Santos Emanuele Giuliani Franciscon Milla Alves Baffi |
| author_sort | Arthur Filipe Sousa Gomes |
| collection | DOAJ |
| description | This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 oC). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts. |
| first_indexed | 2024-12-24T03:10:16Z |
| format | Article |
| id | doaj.art-cab7c2ad265047058379e1721c544b39 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-24T03:10:16Z |
| publishDate | 2016-07-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-cab7c2ad265047058379e1721c544b392022-12-21T17:17:51ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632016-07-0132410.14393/BJ-v32n4a2016-3293532935Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastesArthur Filipe Sousa GomesBruna Silveira Lamanes dos SantosEmanuele Giuliani FrancisconMilla Alves BaffiThis study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 oC). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32935sugarcane bagassewheat breancorn strawxylanaseaspergillus fumigatus |
| spellingShingle | Arthur Filipe Sousa Gomes Bruna Silveira Lamanes dos Santos Emanuele Giuliani Franciscon Milla Alves Baffi Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes Bioscience Journal sugarcane bagasse wheat brean corn straw xylanase aspergillus fumigatus |
| title | Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes |
| title_full | Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes |
| title_fullStr | Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes |
| title_full_unstemmed | Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes |
| title_short | Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes |
| title_sort | substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes |
| topic | sugarcane bagasse wheat brean corn straw xylanase aspergillus fumigatus |
| url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32935 |
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