An insight into the sialotranscriptome and virome of Amazonian anophelines
Abstract Background Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and anti-inflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint a...
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| Format: | Article |
| Language: | English |
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BMC
2019-03-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5545-0 |
| _version_ | 1818684506216857600 |
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| author | Vera Margarete Scarpassa Humbeto Julio Debat Ronildo Baiatone Alencar José Ferreira Saraiva Eric Calvo Bruno Arcà José M. C. Ribeiro |
| author_facet | Vera Margarete Scarpassa Humbeto Julio Debat Ronildo Baiatone Alencar José Ferreira Saraiva Eric Calvo Bruno Arcà José M. C. Ribeiro |
| author_sort | Vera Margarete Scarpassa |
| collection | DOAJ |
| description | Abstract Background Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and anti-inflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies. |
| first_indexed | 2024-12-17T10:51:43Z |
| format | Article |
| id | doaj.art-cab9de5e984041ce96b79f9b86300747 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T10:51:43Z |
| publishDate | 2019-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-cab9de5e984041ce96b79f9b863007472022-12-21T21:51:58ZengBMCBMC Genomics1471-21642019-03-0120111310.1186/s12864-019-5545-0An insight into the sialotranscriptome and virome of Amazonian anophelinesVera Margarete Scarpassa0Humbeto Julio Debat1Ronildo Baiatone Alencar2José Ferreira Saraiva3Eric Calvo4Bruno Arcà5José M. C. Ribeiro6Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da AmazôniaInstituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA)Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da AmazôniaLaboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da AmazôniaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious DiseasesDepartment of Public Health and Infectious Diseases, Division of Parasitology, Sapienza University of RomeLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious DiseasesAbstract Background Saliva of mosquitoes contains anti-platelet, anti-clotting, vasodilatory, anti-complement and anti-inflammatory substances that help the blood feeding process. The salivary polypeptides are at a fast pace of evolution possibly due to their relative lack of structural constraint and possibly also by positive selection on their genes leading to evasion of host immune pressure. Results In this study, we used deep mRNA sequence to uncover for the first time the sialomes of four Amazonian anophelines species (Anopheles braziliensis, A. marajorara, A. nuneztovari and A. triannulatus) and extend the knowledge of the A. darlingi sialome. Two libraries were generated from A. darlingi mosquitoes, sampled from two localities separated ~ 1100 km apart. A total of 60,016 sequences were submitted to GenBank, which will help discovery of novel pharmacologically active polypeptides and the design of specific immunological markers of mosquito exposure. Additionally, in these analyses we identified and characterized novel phasmaviruses and anpheviruses associated to the sialomes of A. triannulatus, A. marajorara and A. darlingi species. Conclusions Besides their pharmacological properties, which may be exploited for the development of new drugs (e.g. anti-thrombotics), salivary proteins of blood feeding arthropods may be turned into tools to prevent and/or better control vector borne diseases; for example, through the development of vaccines or biomarkers to evaluate human exposure to vector bites. The sialotranscriptome study reported here provided novel data on four New World anopheline species and allowed to extend our knowledge on the salivary repertoire of A. darlingi. Additionally, we discovered novel viruses following analysis of the transcriptomes, a procedure that should become standard within future RNAseq studies.http://link.springer.com/article/10.1186/s12864-019-5545-0Vector biologyMosquitoesMalariaVirusSalivary glandsTranscriptome |
| spellingShingle | Vera Margarete Scarpassa Humbeto Julio Debat Ronildo Baiatone Alencar José Ferreira Saraiva Eric Calvo Bruno Arcà José M. C. Ribeiro An insight into the sialotranscriptome and virome of Amazonian anophelines BMC Genomics Vector biology Mosquitoes Malaria Virus Salivary glands Transcriptome |
| title | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_full | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_fullStr | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_full_unstemmed | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_short | An insight into the sialotranscriptome and virome of Amazonian anophelines |
| title_sort | insight into the sialotranscriptome and virome of amazonian anophelines |
| topic | Vector biology Mosquitoes Malaria Virus Salivary glands Transcriptome |
| url | http://link.springer.com/article/10.1186/s12864-019-5545-0 |
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