Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles
Herein we describe the preparation, characterization and the antibacterial effect of Tobramycin-chitosan nanoparticles (TOB-CS NPs) coated with zinc oxide nanoparticles (ZnO NPs). Four formulations of TOB-CS NPs (A-D) were prepared to study the effect of experimental variables on the NPs behavior. T...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016422000238 |
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| author | Nusaiba.K. Al-nemrawi Rami Q. Alkhatib Hadeel Ayyad Nid''A Alshraiedeh |
| author_facet | Nusaiba.K. Al-nemrawi Rami Q. Alkhatib Hadeel Ayyad Nid''A Alshraiedeh |
| author_sort | Nusaiba.K. Al-nemrawi |
| collection | DOAJ |
| description | Herein we describe the preparation, characterization and the antibacterial effect of Tobramycin-chitosan nanoparticles (TOB-CS NPs) coated with zinc oxide nanoparticles (ZnO NPs). Four formulations of TOB-CS NPs (A-D) were prepared to study the effect of experimental variables on the NPs behavior. Two formulations of ZnO NPs were prepared using the solvothermal and the precipitation methods (ZnO1 and ZnO2), and then characterized. TOB-CS NPs (Formula d) was coated with the ZnO1. Moreover, the antibacterial activity of TOB-CS NPs, ZnO NPs and the coated nanoparticles against S. aureus and E. coli was examined. Changing the variables in preparing TOB-CS NPs resulting in variabilities in sizes (297.6–1116.3 nm), charges (+8.29–+39.00 mV), entrapment (51.95–90.60%). Further, TOB release was sustained over four days. ZnO NPs have sizes of 47.44 and 394.4 nm and charges of −62.3 and 89.4 mV when prepared by solvothermal and precipitation technique, respectively. Coated TOB-CS NPs had a size of 342 nm, a charge of +4.39 and released 100 µg/ mL of the drug after four days. The antimicrobial activity of TOB-CS NPs was lower than free TOB against S. aureus and E. coli. The coated NPs showed higher antimicrobial effect in comparison to formula D and ZnO1. In conclusion, coating TOB-CS NPs with ZnO NPs exhibited a great antibacterial effect that may be sustained for days. |
| first_indexed | 2024-12-11T13:13:21Z |
| format | Article |
| id | doaj.art-cabbbb04d01740f284763d5b45022bb5 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-12-11T13:13:21Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-cabbbb04d01740f284763d5b45022bb52022-12-22T01:06:07ZengElsevierSaudi Pharmaceutical Journal1319-01642022-04-01304454461Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticlesNusaiba.K. Al-nemrawi0Rami Q. Alkhatib1Hadeel Ayyad2Nid''A Alshraiedeh3Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Jordan; Corresponding author.Department of Biotechnology and Genetic Engineering, Faculty of Science and Art, Jordan University of Science and Technology, JordanDepartment of Applied Biological Sciences, Faculty of Science and Art, Jordan University of Science and Technology, JordanDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, JordanHerein we describe the preparation, characterization and the antibacterial effect of Tobramycin-chitosan nanoparticles (TOB-CS NPs) coated with zinc oxide nanoparticles (ZnO NPs). Four formulations of TOB-CS NPs (A-D) were prepared to study the effect of experimental variables on the NPs behavior. Two formulations of ZnO NPs were prepared using the solvothermal and the precipitation methods (ZnO1 and ZnO2), and then characterized. TOB-CS NPs (Formula d) was coated with the ZnO1. Moreover, the antibacterial activity of TOB-CS NPs, ZnO NPs and the coated nanoparticles against S. aureus and E. coli was examined. Changing the variables in preparing TOB-CS NPs resulting in variabilities in sizes (297.6–1116.3 nm), charges (+8.29–+39.00 mV), entrapment (51.95–90.60%). Further, TOB release was sustained over four days. ZnO NPs have sizes of 47.44 and 394.4 nm and charges of −62.3 and 89.4 mV when prepared by solvothermal and precipitation technique, respectively. Coated TOB-CS NPs had a size of 342 nm, a charge of +4.39 and released 100 µg/ mL of the drug after four days. The antimicrobial activity of TOB-CS NPs was lower than free TOB against S. aureus and E. coli. The coated NPs showed higher antimicrobial effect in comparison to formula D and ZnO1. In conclusion, coating TOB-CS NPs with ZnO NPs exhibited a great antibacterial effect that may be sustained for days.http://www.sciencedirect.com/science/article/pii/S1319016422000238TobramycinNanoparticlesIonic gelationTPPZinc oxideBacterial resistance |
| spellingShingle | Nusaiba.K. Al-nemrawi Rami Q. Alkhatib Hadeel Ayyad Nid''A Alshraiedeh Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles Saudi Pharmaceutical Journal Tobramycin Nanoparticles Ionic gelation TPP Zinc oxide Bacterial resistance |
| title | Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles |
| title_full | Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles |
| title_fullStr | Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles |
| title_full_unstemmed | Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles |
| title_short | Formulation and characterization of tobramycin-chitosan nanoparticles coated with zinc oxide nanoparticles |
| title_sort | formulation and characterization of tobramycin chitosan nanoparticles coated with zinc oxide nanoparticles |
| topic | Tobramycin Nanoparticles Ionic gelation TPP Zinc oxide Bacterial resistance |
| url | http://www.sciencedirect.com/science/article/pii/S1319016422000238 |
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