Does MiRNA gene expression predict gastric cancer stage and localization?
Gastric cancer comprises 10% of all cancers in the world and is rarely seen before the age of 30. Different genetic interactions or molecular changes like e-cadherin, P16, APC, c-erb B2, c-met, ras, or c-myc have been described in the pathogenesis of the disease. MiRNAs play critical roles in variou...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2019-03-01
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| Series: | Medicine Science |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=27512 |
| _version_ | 1797329182563237888 |
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| author | Onder Ozcan Ahmet Korkut Belli Selami Ilgaz Kayilioglu Ozgur Ilhan Celik Mehmet Kaplan Murat Kara Murat Polat |
| author_facet | Onder Ozcan Ahmet Korkut Belli Selami Ilgaz Kayilioglu Ozgur Ilhan Celik Mehmet Kaplan Murat Kara Murat Polat |
| author_sort | Onder Ozcan |
| collection | DOAJ |
| description | Gastric cancer comprises 10% of all cancers in the world and is rarely seen before the age of 30. Different genetic interactions or molecular changes like e-cadherin, P16, APC, c-erb B2, c-met, ras, or c-myc have been described in the pathogenesis of the disease. MiRNAs play critical roles in various biological processes such as cell proliferation, cell differentiation, apoptosis, repairing of DNA damage, angiogenesis, stress response, and stem cell division. The aim of this study is to investigate the relationship between miRNA gene expressions and gastric cancer. Method: Sixty-four patients with gastric cancer diagnosed consecutively in our pathology laboratory and 30 consecutive participants with normal gastric tissue, as a control group, were included in our study between 2011 and 2013. Total miRNAs were obtained by using genomic miRNA extraction kit (QIAGEN Sample & Assay Technologies, Hilden, Germany) after the samples had been deparaffinized. MiR-21, 107, 141, 150, 192, 202, 218, 331 gene expressions were evaluated by using real time-PCR (RT-PCR) from the whole miRNA. Results: MiR-331 was downregulated in the stage 2 patients and the rest of the miRNAs were significantly upregulated (p < 0.001). All miRNAs were significantly upregulated in the stage 3a patients (p < 0.001). In the antrum localized tumors, miR-107, -150, -202, -218, and -331 were significantly upregulated, whereas miR-21, -192 were statistically downregulated (p < 0.05). In the cardia localized tumors, miR-21 (p > 0.05) was downregulated and miR-107, -150, -202, -218, and -331 were upregulated (p < 0.05) Different gene expressions of miRNAs according to the different localizations of gastric cancer and different stages suggest that it can be tumor marker to predict the stage and localization of gastric cancer. [Med-Science 2019; 8(1.000): 255-9] |
| first_indexed | 2024-03-08T07:02:08Z |
| format | Article |
| id | doaj.art-cabbf77a3d754e488a918417b55e9ac8 |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T07:02:08Z |
| publishDate | 2019-03-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-cabbf77a3d754e488a918417b55e9ac82024-02-03T05:37:14ZengSociety of Turaz BilimMedicine Science2147-06342019-03-0181255910.5455/medscience.2019.08.901227512Does MiRNA gene expression predict gastric cancer stage and localization?Onder Ozcan0Ahmet Korkut BelliSelami Ilgaz KayiliogluOzgur Ilhan CelikMehmet KaplanMurat KaraMurat PolatMugla Sitki Kocman Universty Medical School, Department of General Surgery, Mugla, Turkey . Mugla Sitki Kocman Universty Medical School, Department of Pathology, Mugla, Turkey. NCR International Hospital, Department of General Surgery, Gaziantep, Turkey. Medical Genetics Specialist, Self Employment, Mersin,Turkey. Mugla Sitki Kocman Universty Medical School, Department of General Surgery, Mugla, TurkeyGastric cancer comprises 10% of all cancers in the world and is rarely seen before the age of 30. Different genetic interactions or molecular changes like e-cadherin, P16, APC, c-erb B2, c-met, ras, or c-myc have been described in the pathogenesis of the disease. MiRNAs play critical roles in various biological processes such as cell proliferation, cell differentiation, apoptosis, repairing of DNA damage, angiogenesis, stress response, and stem cell division. The aim of this study is to investigate the relationship between miRNA gene expressions and gastric cancer. Method: Sixty-four patients with gastric cancer diagnosed consecutively in our pathology laboratory and 30 consecutive participants with normal gastric tissue, as a control group, were included in our study between 2011 and 2013. Total miRNAs were obtained by using genomic miRNA extraction kit (QIAGEN Sample & Assay Technologies, Hilden, Germany) after the samples had been deparaffinized. MiR-21, 107, 141, 150, 192, 202, 218, 331 gene expressions were evaluated by using real time-PCR (RT-PCR) from the whole miRNA. Results: MiR-331 was downregulated in the stage 2 patients and the rest of the miRNAs were significantly upregulated (p < 0.001). All miRNAs were significantly upregulated in the stage 3a patients (p < 0.001). In the antrum localized tumors, miR-107, -150, -202, -218, and -331 were significantly upregulated, whereas miR-21, -192 were statistically downregulated (p < 0.05). In the cardia localized tumors, miR-21 (p > 0.05) was downregulated and miR-107, -150, -202, -218, and -331 were upregulated (p < 0.05) Different gene expressions of miRNAs according to the different localizations of gastric cancer and different stages suggest that it can be tumor marker to predict the stage and localization of gastric cancer. [Med-Science 2019; 8(1.000): 255-9]http://www.ejmanager.com/fulltextpdf.php?mno=27512MiRNAgastric carcinomageneexpression |
| spellingShingle | Onder Ozcan Ahmet Korkut Belli Selami Ilgaz Kayilioglu Ozgur Ilhan Celik Mehmet Kaplan Murat Kara Murat Polat Does MiRNA gene expression predict gastric cancer stage and localization? Medicine Science MiRNA gastric carcinoma gene expression |
| title | Does MiRNA gene expression predict gastric cancer stage and localization? |
| title_full | Does MiRNA gene expression predict gastric cancer stage and localization? |
| title_fullStr | Does MiRNA gene expression predict gastric cancer stage and localization? |
| title_full_unstemmed | Does MiRNA gene expression predict gastric cancer stage and localization? |
| title_short | Does MiRNA gene expression predict gastric cancer stage and localization? |
| title_sort | does mirna gene expression predict gastric cancer stage and localization |
| topic | MiRNA gastric carcinoma gene expression |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=27512 |
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