An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022
In August 2022 on a backyard farm in the Moscow region of Russia, mortality was observed among chickens, and all 45 birds of a particular farm died or were slaughtered after the onset of symptoms within a few days. Paramyxovirus was isolated from the diseased birds. Based on the nucleotide sequences...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/10/6/404 |
| _version_ | 1797592395202691072 |
|---|---|
| author | Artyom Rtishchev Anastasia Treshchalina Elena Shustova Elizaveta Boravleva Alexandra Gambaryan |
| author_facet | Artyom Rtishchev Anastasia Treshchalina Elena Shustova Elizaveta Boravleva Alexandra Gambaryan |
| author_sort | Artyom Rtishchev |
| collection | DOAJ |
| description | In August 2022 on a backyard farm in the Moscow region of Russia, mortality was observed among chickens, and all 45 birds of a particular farm died or were slaughtered after the onset of symptoms within a few days. Paramyxovirus was isolated from the diseased birds. Based on the nucleotide sequences of the F and NP gene fragments, it was determined that the virus belonged to subgenotype VII.1 AAvV-1 class II. The cleavage site of the F gene <sub>109</sub>SGGRRQKRFIG<sub>119</sub> and T in 546 and 555 position of the NP gene were typical for the velogenic type. The genetically closest NDV isolates were found in Iran. The mean time of death of 10-day-old chicken embryos upon infection with the minimal infectious dose was 52 h, which is typical for the velogenic pathotype. The virus caused 100% death of six-week-old chickens during oral infection as well as 100% mortality of all contact chickens, including those located in remote cages, which proves the ability of the virus to spread not only by the fecal–oral route but also by the aerosol route. That demonstrates a high level of pathogenicity and contagiousness of the isolated strain for chicken. However, mice intranasally infected with high doses of the virus did not die. |
| first_indexed | 2024-03-11T01:51:22Z |
| format | Article |
| id | doaj.art-cabc3141575649b2994a883fa3f090c7 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T01:51:22Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-cabc3141575649b2994a883fa3f090c72023-11-18T13:00:26ZengMDPI AGVeterinary Sciences2306-73812023-06-0110640410.3390/vetsci10060404An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022Artyom Rtishchev0Anastasia Treshchalina1Elena Shustova2Elizaveta Boravleva3Alexandra Gambaryan4Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera», 105064 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaChumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement “Moskovskiy”, 108819 Moscow, RussiaIn August 2022 on a backyard farm in the Moscow region of Russia, mortality was observed among chickens, and all 45 birds of a particular farm died or were slaughtered after the onset of symptoms within a few days. Paramyxovirus was isolated from the diseased birds. Based on the nucleotide sequences of the F and NP gene fragments, it was determined that the virus belonged to subgenotype VII.1 AAvV-1 class II. The cleavage site of the F gene <sub>109</sub>SGGRRQKRFIG<sub>119</sub> and T in 546 and 555 position of the NP gene were typical for the velogenic type. The genetically closest NDV isolates were found in Iran. The mean time of death of 10-day-old chicken embryos upon infection with the minimal infectious dose was 52 h, which is typical for the velogenic pathotype. The virus caused 100% death of six-week-old chickens during oral infection as well as 100% mortality of all contact chickens, including those located in remote cages, which proves the ability of the virus to spread not only by the fecal–oral route but also by the aerosol route. That demonstrates a high level of pathogenicity and contagiousness of the isolated strain for chicken. However, mice intranasally infected with high doses of the virus did not die.https://www.mdpi.com/2306-7381/10/6/404NDVParamyxovirusoutbreakpathogenicity |
| spellingShingle | Artyom Rtishchev Anastasia Treshchalina Elena Shustova Elizaveta Boravleva Alexandra Gambaryan An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022 Veterinary Sciences NDV Paramyxovirus outbreak pathogenicity |
| title | An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022 |
| title_full | An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022 |
| title_fullStr | An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022 |
| title_full_unstemmed | An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022 |
| title_short | An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022 |
| title_sort | outbreak of newcastle disease virus in the moscow region in the summer of 2022 |
| topic | NDV Paramyxovirus outbreak pathogenicity |
| url | https://www.mdpi.com/2306-7381/10/6/404 |
| work_keys_str_mv | AT artyomrtishchev anoutbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT anastasiatreshchalina anoutbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT elenashustova anoutbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT elizavetaboravleva anoutbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT alexandragambaryan anoutbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT artyomrtishchev outbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT anastasiatreshchalina outbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT elenashustova outbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT elizavetaboravleva outbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 AT alexandragambaryan outbreakofnewcastlediseasevirusinthemoscowregioninthesummerof2022 |