Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers
A colloidal particle placed inside the cell cytoplasm is enmeshed within a network of cytoskeletal fibres immersed in the cytosolic fluid. The translational mode is believed to yield different rheological parameters than the rotational mode, given that these modes stretch the fibers differently. We...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Physics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphy.2022.1099958/full |
| _version_ | 1828068557716455424 |
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| author | Srestha Roy Rahul Vaippully Muruga Lokesh Gokul Nalupurackal Privita Edwina Saumendra Bajpai Basudev Roy |
| author_facet | Srestha Roy Rahul Vaippully Muruga Lokesh Gokul Nalupurackal Privita Edwina Saumendra Bajpai Basudev Roy |
| author_sort | Srestha Roy |
| collection | DOAJ |
| description | A colloidal particle placed inside the cell cytoplasm is enmeshed within a network of cytoskeletal fibres immersed in the cytosolic fluid. The translational mode is believed to yield different rheological parameters than the rotational mode, given that these modes stretch the fibers differently. We compare the parameters for Michigan Cancer Foundation-7 (MCF-7) cells in this manuscript and find that the results are well comparable to each other. At low values of 0 Hz viscosity, the rotational and translational viscoelasticity matches well. However, discrepancies appear at higher values which may indicate that the cytoskeletal modes involved in rotation and translation of the particle are getting invoked. We also show that the 0 Hz viscosity increases as the cell ages under the conditions of constant room temperature of 25°C on the sample chamber. |
| first_indexed | 2024-04-11T00:04:49Z |
| format | Article |
| id | doaj.art-cabea8faa3f34ecba4bb505d9d18e420 |
| institution | Directory Open Access Journal |
| issn | 2296-424X |
| language | English |
| last_indexed | 2024-04-11T00:04:49Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physics |
| spelling | doaj.art-cabea8faa3f34ecba4bb505d9d18e4202023-01-09T12:21:31ZengFrontiers Media S.A.Frontiers in Physics2296-424X2023-01-011010.3389/fphy.2022.10999581099958Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezersSrestha Roy0Rahul Vaippully1Muruga Lokesh2Gokul Nalupurackal3Privita Edwina4Saumendra Bajpai5Basudev Roy6Department of Physics, Indian Institute of Technology Madras, Chennai, IndiaDepartment of Physics, Indian Institute of Technology Madras, Chennai, IndiaDepartment of Physics, Indian Institute of Technology Madras, Chennai, IndiaDepartment of Physics, Indian Institute of Technology Madras, Chennai, IndiaDepartment of Applied Mechanics, Indian Institute of Technology Madras, Chennai, IndiaDepartment of Applied Mechanics, Indian Institute of Technology Madras, Chennai, IndiaDepartment of Physics, Indian Institute of Technology Madras, Chennai, IndiaA colloidal particle placed inside the cell cytoplasm is enmeshed within a network of cytoskeletal fibres immersed in the cytosolic fluid. The translational mode is believed to yield different rheological parameters than the rotational mode, given that these modes stretch the fibers differently. We compare the parameters for Michigan Cancer Foundation-7 (MCF-7) cells in this manuscript and find that the results are well comparable to each other. At low values of 0 Hz viscosity, the rotational and translational viscoelasticity matches well. However, discrepancies appear at higher values which may indicate that the cytoskeletal modes involved in rotation and translation of the particle are getting invoked. We also show that the 0 Hz viscosity increases as the cell ages under the conditions of constant room temperature of 25°C on the sample chamber.https://www.frontiersin.org/articles/10.3389/fphy.2022.1099958/fullviscosity ratiorelaxation timecytosoltranslationrotationoptical tweezers |
| spellingShingle | Srestha Roy Rahul Vaippully Muruga Lokesh Gokul Nalupurackal Privita Edwina Saumendra Bajpai Basudev Roy Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers Frontiers in Physics viscosity ratio relaxation time cytosol translation rotation optical tweezers |
| title | Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers |
| title_full | Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers |
| title_fullStr | Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers |
| title_full_unstemmed | Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers |
| title_short | Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers |
| title_sort | comparison of translational and rotational modes towards passive rheology of the cytoplasm of mcf 7 cells using optical tweezers |
| topic | viscosity ratio relaxation time cytosol translation rotation optical tweezers |
| url | https://www.frontiersin.org/articles/10.3389/fphy.2022.1099958/full |
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