In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine
Background: Thalictrum minus L. is a medicinal plant contains berberine, which is used for complementary treating of some diseases. Excessive harvesting of this species from rangelands to extract the alkaloid has endangered its survival. Objective: This study was aimed for in vitro propagation of th...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2022-03-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-3218-en.html |
| _version_ | 1818041726029266944 |
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| author | Zahra Sargazi Moghadam Nasrin Moshtaghi Ahmad Sharifi Asma Zahedi Azade Khadem |
| author_facet | Zahra Sargazi Moghadam Nasrin Moshtaghi Ahmad Sharifi Asma Zahedi Azade Khadem |
| author_sort | Zahra Sargazi Moghadam |
| collection | DOAJ |
| description | Background: Thalictrum minus L. is a medicinal plant contains berberine, which is used for complementary treating of some diseases. Excessive harvesting of this species from rangelands to extract the alkaloid has endangered its survival. Objective: This study was aimed for in vitro propagation of this species and berberine production. Methods: The seeds were germinated at in vitro culture conditions. Then, the leaves were used as explants for callus induction in two MS and LS basal media supplemented with BA (0, 0.1, 0.5 and 1 mg/L) in combination with different concentrations of auxins, NAA, 2,4-D or IAA. Results: The results showed that MS medium with the plant growth regulator combination of 0.5 mg/L BA and 0.5 mg/L NAA had the highest callus formation and 100 % regeneration. Some calli were transferred to three liquid culture media (MS, LS, or ½ MS) with 1 mg/L BA + 0.1 mg/L NAA or without plant growth regulator to produce the berberine. The HPLC results identified 6 % of berberine in the liquid medium. This amount of berberine can be easily released into the culture medium, therefore, it will be easier to extract. Conclusion: This method can be successfully used for propagation and extraction of berberine without plant destruction. |
| first_indexed | 2024-12-10T08:35:00Z |
| format | Article |
| id | doaj.art-cabfd9ca29874aeb8b25fef13ad31c15 |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-10T08:35:00Z |
| publishDate | 2022-03-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-cabfd9ca29874aeb8b25fef13ad31c152022-12-22T01:56:00ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582022-03-01218192101In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberineZahra Sargazi Moghadam0Nasrin Moshtaghi1Ahmad Sharifi2Asma Zahedi3Azade Khadem4 Department of Agricultural Biotechnology, Ferdowsi University of Mashhad, Iran Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Iran Academic Center for Industrial Biotechnology, ACECR, Branch of Khorasan Razavi, Mashhad, Iran Department of Agricultural Biotechnology, Ferdowsi University of Mashhad, Iran Academic Center for Industrial Biotechnology, ACECR, Branch of Khorasan Razavi, Mashhad, Iran Background: Thalictrum minus L. is a medicinal plant contains berberine, which is used for complementary treating of some diseases. Excessive harvesting of this species from rangelands to extract the alkaloid has endangered its survival. Objective: This study was aimed for in vitro propagation of this species and berberine production. Methods: The seeds were germinated at in vitro culture conditions. Then, the leaves were used as explants for callus induction in two MS and LS basal media supplemented with BA (0, 0.1, 0.5 and 1 mg/L) in combination with different concentrations of auxins, NAA, 2,4-D or IAA. Results: The results showed that MS medium with the plant growth regulator combination of 0.5 mg/L BA and 0.5 mg/L NAA had the highest callus formation and 100 % regeneration. Some calli were transferred to three liquid culture media (MS, LS, or ½ MS) with 1 mg/L BA + 0.1 mg/L NAA or without plant growth regulator to produce the berberine. The HPLC results identified 6 % of berberine in the liquid medium. This amount of berberine can be easily released into the culture medium, therefore, it will be easier to extract. Conclusion: This method can be successfully used for propagation and extraction of berberine without plant destruction.http://jmp.ir/article-1-3218-en.htmlthalictrum minusalkaloidsin vitroextractionsecondary metabolite |
| spellingShingle | Zahra Sargazi Moghadam Nasrin Moshtaghi Ahmad Sharifi Asma Zahedi Azade Khadem In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine Journal of Medicinal Plants thalictrum minus alkaloids in vitro extraction secondary metabolite |
| title | In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine |
| title_full | In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine |
| title_fullStr | In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine |
| title_full_unstemmed | In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine |
| title_short | In vitro propagation and callus induction of medicinal endangered plant meadow rue (Thalictrum minus L.) for producing berberine |
| title_sort | in vitro propagation and callus induction of medicinal endangered plant meadow rue thalictrum minus l for producing berberine |
| topic | thalictrum minus alkaloids in vitro extraction secondary metabolite |
| url | http://jmp.ir/article-1-3218-en.html |
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