Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS
Yeonkyopaedok-san is a traditional Korean medicine used in the early treatment of boils. In the present study, its 12 marker components for quality control were determined using high-performance liquid chromatography (HPLC) with photodiode array detection and ultra-performance liquid chromatography&...
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MDPI AG
2020-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/5/1713 |
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| author | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_facet | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_sort | Chang-Seob Seo |
| collection | DOAJ |
| description | Yeonkyopaedok-san is a traditional Korean medicine used in the early treatment of boils. In the present study, its 12 marker components for quality control were determined using high-performance liquid chromatography (HPLC) with photodiode array detection and ultra-performance liquid chromatography−mass spectrometry with tandem mass spectrometry (UPLC−MS/MS). The investigated 12 marker components of Yeonkyopaedok-san were as follows: 3-caffeoylquinic acid, cimifugin 7-glucoside, liquiritin apioside, ferulic acid, narirutin, 5-<i>O</i>-methylvisammioside, naringin, neohesperidin, oxypeucedanin hydrate, arctigenin, glycyrrhizic acid, and 6-gingerol. The analytical column used for the separation of the 12 marker analytes in Yeonkyopaedok-san was a Waters SunFire C18 column (4.6 mm × 250 mm, 5 μm). The two mobile phases used were 0.1% (<i>v</i>/<i>v</i>) aqueous formic acid and 0.1% (<i>v</i>/<i>v</i>) formic acid in acetonitrile. In the UPLC−MS/MS analysis, all components were separated using a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm). The two mobile phases used were 0.1% (<i>v</i>/<i>v</i>) aqueous formic acid and acetonitrile. The coefficient of determination of the calibration curves in both analysis systems showed good linearity (>0.99). The amounts of the 12 marker components in Yeonkyopaedok-san determined using HPLC−photodiode array detection and UPLC−MS/MS analyses were found to be 0.14−9.00 mg/g and 2.35−853.11 μg/g, respectively. |
| first_indexed | 2024-12-13T06:31:10Z |
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| language | English |
| last_indexed | 2024-12-13T06:31:10Z |
| publishDate | 2020-03-01 |
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| series | Applied Sciences |
| spelling | doaj.art-cac1505562b64c0c934f96082edccd9f2022-12-21T23:56:37ZengMDPI AGApplied Sciences2076-34172020-03-01105171310.3390/app10051713app10051713Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MSChang-Seob Seo0Hyeun-Kyoo Shin1Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaYeonkyopaedok-san is a traditional Korean medicine used in the early treatment of boils. In the present study, its 12 marker components for quality control were determined using high-performance liquid chromatography (HPLC) with photodiode array detection and ultra-performance liquid chromatography−mass spectrometry with tandem mass spectrometry (UPLC−MS/MS). The investigated 12 marker components of Yeonkyopaedok-san were as follows: 3-caffeoylquinic acid, cimifugin 7-glucoside, liquiritin apioside, ferulic acid, narirutin, 5-<i>O</i>-methylvisammioside, naringin, neohesperidin, oxypeucedanin hydrate, arctigenin, glycyrrhizic acid, and 6-gingerol. The analytical column used for the separation of the 12 marker analytes in Yeonkyopaedok-san was a Waters SunFire C18 column (4.6 mm × 250 mm, 5 μm). The two mobile phases used were 0.1% (<i>v</i>/<i>v</i>) aqueous formic acid and 0.1% (<i>v</i>/<i>v</i>) formic acid in acetonitrile. In the UPLC−MS/MS analysis, all components were separated using a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm). The two mobile phases used were 0.1% (<i>v</i>/<i>v</i>) aqueous formic acid and acetonitrile. The coefficient of determination of the calibration curves in both analysis systems showed good linearity (>0.99). The amounts of the 12 marker components in Yeonkyopaedok-san determined using HPLC−photodiode array detection and UPLC−MS/MS analyses were found to be 0.14−9.00 mg/g and 2.35−853.11 μg/g, respectively.https://www.mdpi.com/2076-3417/10/5/1713hplc–pdalc–ms/mssimultaneous determinationtraditional herbal formulayeonkyopaedok-san |
| spellingShingle | Chang-Seob Seo Hyeun-Kyoo Shin Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS Applied Sciences hplc–pda lc–ms/ms simultaneous determination traditional herbal formula yeonkyopaedok-san |
| title | Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS |
| title_full | Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS |
| title_fullStr | Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS |
| title_full_unstemmed | Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS |
| title_short | Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS |
| title_sort | simultaneous determination of 12 marker components in yeonkyopaedok san using hplc pda and lc ms ms |
| topic | hplc–pda lc–ms/ms simultaneous determination traditional herbal formula yeonkyopaedok-san |
| url | https://www.mdpi.com/2076-3417/10/5/1713 |
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