Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
Intracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important cha...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977129/?tool=EBI |
| _version_ | 1818404629130510336 |
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| author | Mitsuru J Nakamura Kohji Hotta Kotaro Oka |
| author_facet | Mitsuru J Nakamura Kohji Hotta Kotaro Oka |
| author_sort | Mitsuru J Nakamura |
| collection | DOAJ |
| description | Intracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important challenge in developmental biology. Raman spectroscopic imaging, a non-invasive and label-free technique, allows simultaneous imaging of the intracellular composition and distribution of multiple bio-molecules. In this study, we explored the application of Raman spectroscopic imaging in the whole Ciona intestinalis embryo during development. Analysis of Raman spectra scattered from C. intestinalis embryos revealed a number of localized patterns of high Raman intensity within the embryo. Based on the observed distribution of bio-molecules, we succeeded in identifying the location and structure of differentiated muscle and endoderm within the whole embryo, up to the tailbud stage, in a label-free manner. Furthermore, during cell differentiation, we detected significant differences in cell state between muscle/endoderm daughter cells and daughter cells with other fates that had divided from the same mother cells; this was achieved by focusing on the Raman intensity of single Raman bands at 1002 or 1526 cm(-1), respectively. This study reports the first application of Raman spectroscopic imaging to the study of identifying and characterizing differentiating tissues in a whole chordate embryo. Our results suggest that Raman spectroscopic imaging is a feasible label-free technique for investigating the developmental process of the whole embryo of C. intestinalis. |
| first_indexed | 2024-12-14T08:43:11Z |
| format | Article |
| id | doaj.art-cac2801dbf024287b36108ecc7dc0019 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:43:11Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cac2801dbf024287b36108ecc7dc00192022-12-21T23:09:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7173910.1371/journal.pone.0071739Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.Mitsuru J NakamuraKohji HottaKotaro OkaIntracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important challenge in developmental biology. Raman spectroscopic imaging, a non-invasive and label-free technique, allows simultaneous imaging of the intracellular composition and distribution of multiple bio-molecules. In this study, we explored the application of Raman spectroscopic imaging in the whole Ciona intestinalis embryo during development. Analysis of Raman spectra scattered from C. intestinalis embryos revealed a number of localized patterns of high Raman intensity within the embryo. Based on the observed distribution of bio-molecules, we succeeded in identifying the location and structure of differentiated muscle and endoderm within the whole embryo, up to the tailbud stage, in a label-free manner. Furthermore, during cell differentiation, we detected significant differences in cell state between muscle/endoderm daughter cells and daughter cells with other fates that had divided from the same mother cells; this was achieved by focusing on the Raman intensity of single Raman bands at 1002 or 1526 cm(-1), respectively. This study reports the first application of Raman spectroscopic imaging to the study of identifying and characterizing differentiating tissues in a whole chordate embryo. Our results suggest that Raman spectroscopic imaging is a feasible label-free technique for investigating the developmental process of the whole embryo of C. intestinalis.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977129/?tool=EBI |
| spellingShingle | Mitsuru J Nakamura Kohji Hotta Kotaro Oka Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. PLoS ONE |
| title | Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. |
| title_full | Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. |
| title_fullStr | Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. |
| title_full_unstemmed | Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. |
| title_short | Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development. |
| title_sort | raman spectroscopic imaging of the whole ciona intestinalis embryo during development |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977129/?tool=EBI |
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