Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review
Abstract In situ physiological signals of in vitro neural disease models are essential for studying pathogenesis and drug screening. Currently, an increasing number of in vitro neural disease models are established using human‐induced pluripotent stem cell (hiPSC) derived neurons (hiPSC‐DNs) to over...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202301828 |
| _version_ | 1797465055266078720 |
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| author | Shiya Lv Enhui He Jinping Luo Yaoyao Liu Wei Liang Shihong Xu Kui Zhang Yan Yang Mixia Wang Yilin Song Yirong Wu Xinxia Cai |
| author_facet | Shiya Lv Enhui He Jinping Luo Yaoyao Liu Wei Liang Shihong Xu Kui Zhang Yan Yang Mixia Wang Yilin Song Yirong Wu Xinxia Cai |
| author_sort | Shiya Lv |
| collection | DOAJ |
| description | Abstract In situ physiological signals of in vitro neural disease models are essential for studying pathogenesis and drug screening. Currently, an increasing number of in vitro neural disease models are established using human‐induced pluripotent stem cell (hiPSC) derived neurons (hiPSC‐DNs) to overcome interspecific gene expression differences. Microelectrode arrays (MEAs) can be readily interfaced with two‐dimensional (2D), and more recently, three‐dimensional (3D) neural stem cell‐derived in vitro models of the human brain to monitor their physiological activity in real time. Therefore, MEAs are emerging and useful tools to model neurological disorders and disease in vitro using human iPSCs. This is enabling a real‐time window into neuronal signaling at the network scale from patient derived. This paper provides a comprehensive review of MEA's role in analyzing neural disease models established by hiPSC‐DNs. It covers the significance of MEA fabrication, surface structure and modification schemes for hiPSC‐DNs culturing and signal detection. Additionally, this review discusses advances in the development and use of MEA technology to study in vitro neural disease models, including epilepsy, autism spectrum developmental disorder (ASD), and others established using hiPSC‐DNs. The paper also highlights the application of MEAs combined with hiPSC‐DNs in detecting in vitro neurotoxic substances. Finally, the future development and outlook of multifunctional and integrated devices for in vitro medical diagnostics and treatment are discussed. |
| first_indexed | 2024-03-09T18:17:02Z |
| format | Article |
| id | doaj.art-cac2b3bd28884a048a0e4a1e099f035b |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-09T18:17:02Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-cac2b3bd28884a048a0e4a1e099f035b2023-11-24T08:40:29ZengWileyAdvanced Science2198-38442023-11-011033n/an/a10.1002/advs.202301828Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A ReviewShiya Lv0Enhui He1Jinping Luo2Yaoyao Liu3Wei Liang4Shihong Xu5Kui Zhang6Yan Yang7Mixia Wang8Yilin Song9Yirong Wu10Xinxia Cai11State Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaState Key Laboratory of Transducer Technology Aerospace Information Research Institute Chinese Academy of Sciences Beijing 100190 ChinaAbstract In situ physiological signals of in vitro neural disease models are essential for studying pathogenesis and drug screening. Currently, an increasing number of in vitro neural disease models are established using human‐induced pluripotent stem cell (hiPSC) derived neurons (hiPSC‐DNs) to overcome interspecific gene expression differences. Microelectrode arrays (MEAs) can be readily interfaced with two‐dimensional (2D), and more recently, three‐dimensional (3D) neural stem cell‐derived in vitro models of the human brain to monitor their physiological activity in real time. Therefore, MEAs are emerging and useful tools to model neurological disorders and disease in vitro using human iPSCs. This is enabling a real‐time window into neuronal signaling at the network scale from patient derived. This paper provides a comprehensive review of MEA's role in analyzing neural disease models established by hiPSC‐DNs. It covers the significance of MEA fabrication, surface structure and modification schemes for hiPSC‐DNs culturing and signal detection. Additionally, this review discusses advances in the development and use of MEA technology to study in vitro neural disease models, including epilepsy, autism spectrum developmental disorder (ASD), and others established using hiPSC‐DNs. The paper also highlights the application of MEAs combined with hiPSC‐DNs in detecting in vitro neurotoxic substances. Finally, the future development and outlook of multifunctional and integrated devices for in vitro medical diagnostics and treatment are discussed.https://doi.org/10.1002/advs.202301828CMOS‐MEAhuman‐induced pluripotent stem cell‐derived neurons (hiPSC‐DNs)in vitro brain‐machine interface (in BMI)microelectrode arrays (MEAs)neural diseasesneurotoxic substances |
| spellingShingle | Shiya Lv Enhui He Jinping Luo Yaoyao Liu Wei Liang Shihong Xu Kui Zhang Yan Yang Mixia Wang Yilin Song Yirong Wu Xinxia Cai Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review Advanced Science CMOS‐MEA human‐induced pluripotent stem cell‐derived neurons (hiPSC‐DNs) in vitro brain‐machine interface (in BMI) microelectrode arrays (MEAs) neural diseases neurotoxic substances |
| title | Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review |
| title_full | Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review |
| title_fullStr | Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review |
| title_full_unstemmed | Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review |
| title_short | Using Human‐Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review |
| title_sort | using human induced pluripotent stem cell derived neurons on microelectrode arrays to model neurological disease a review |
| topic | CMOS‐MEA human‐induced pluripotent stem cell‐derived neurons (hiPSC‐DNs) in vitro brain‐machine interface (in BMI) microelectrode arrays (MEAs) neural diseases neurotoxic substances |
| url | https://doi.org/10.1002/advs.202301828 |
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