Production of Poly--hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
<!--[if gte mso 9]><xml> <o:DocumentProperties> <o:Description>generated by an Adobe application</o:Description> <o:Version>11.5606</o:Version> </o:DocumentProperties> </xml><![endif]--> <p class="MsoNormal" style="text-al...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2015-11-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/7804 |
| Summary: | <!--[if gte mso 9]><xml> <o:DocumentProperties> <o:Description>generated by an Adobe application</o:Description> <o:Version>11.5606</o:Version> </o:DocumentProperties> </xml><![endif]--> <p class="MsoNormal" style="text-align: justify; text-justify: inter-ideograph; mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 9.0pt; font-family: "Book Antiqua"; mso-fareast-font-family: "MS Mincho"; mso-bidi-font-family: "Book Antiqua"; color: black; mso-ansi-language: EN-GB; mso-fareast-language: JA;">A new bacterial strain that produces amylase and poly-</span><span style="font-size: 9.0pt; font-family: Symbol; mso-fareast-font-family: "MS Mincho"; mso-bidi-font-family: Symbol; color: black; mso-ansi-language: EN-GB; mso-fareast-language: JA;">a</span><span style="font-size: 9.0pt; font-family: "Book Antiqua"; mso-fareast-font-family: "MS Mincho"; mso-bidi-font-family: "Book Antiqua"; color: black; mso-ansi-language: EN-GB; mso-fareast-language: JA;">-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the <em>Bacillus megaterium</em> group based on phenotypic characteristics<span style="mso-spacerun: yes;"> </span>and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by <em>B. megaterium </em>PSA10 was identified by<span style="mso-spacerun: yes;"> </span>Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain <em>B.megaterium</em> PSA10 was a potent PHB producer.</span></p> <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:HyphenationZone>36</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:UseFELayout /> </w:Compatibility> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} --> <!--[endif] --> |
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| ISSN: | 0853-8654 2089-2241 |