First Look at the Venom of Naja ashei
Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/3/609 |
| _version_ | 1811274972142764032 |
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| author | Konrad Kamil Hus Justyna Buczkowicz Vladimír Petrilla Monika Petrillová Andrzej Łyskowski Jaroslav Legáth Aleksandra Bocian |
| author_facet | Konrad Kamil Hus Justyna Buczkowicz Vladimír Petrilla Monika Petrillová Andrzej Łyskowski Jaroslav Legáth Aleksandra Bocian |
| author_sort | Konrad Kamil Hus |
| collection | DOAJ |
| description | Naja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA2s (Phospholipases A2). Additionally the presence of cysteine-rich venom proteins, 5′-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras—cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species. |
| first_indexed | 2024-04-12T23:29:50Z |
| format | Article |
| id | doaj.art-cac6bffda9d3417883fe92173da68375 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T23:29:50Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cac6bffda9d3417883fe92173da683752022-12-22T03:12:19ZengMDPI AGMolecules1420-30492018-03-0123360910.3390/molecules23030609molecules23030609First Look at the Venom of Naja asheiKonrad Kamil Hus0Justyna Buczkowicz1Vladimír Petrilla2Monika Petrillová3Andrzej Łyskowski4Jaroslav Legáth5Aleksandra Bocian6Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, PolandDepartment of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, SlovakiaDepartment of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, SlovakiaDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, PolandDepartment of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszow, PolandNaja ashei is an African spitting cobra species closely related to N. mossambica and N. nigricollis. It is known that the venom of N. ashei, like that of other African spitting cobras, mainly has cytotoxic effects, however data about its specific protein composition are not yet available. Thus, an attempt was made to determine the venom proteome of N. ashei with the use of 2-D electrophoresis and MALDI ToF/ToF (Matrix-Assisted Laser Desorption/Ionization Time of Flight) mass spectrometry techniques. Our investigation revealed that the main components of analysed venom are 3FTxs (Three-Finger Toxins) and PLA2s (Phospholipases A2). Additionally the presence of cysteine-rich venom proteins, 5′-nucleotidase and metalloproteinases has also been confirmed. The most interesting fact derived from this study is that the venom of N. ashei includes proteins not described previously in other African spitting cobras—cobra venom factor and venom nerve growth factor. To our knowledge, there are currently no other reports concerning this venom composition and we believe that our results will significantly increase interest in research of this species.http://www.mdpi.com/1420-3049/23/3/609Naja asheivenom composition2-D electrophoresisproteomics |
| spellingShingle | Konrad Kamil Hus Justyna Buczkowicz Vladimír Petrilla Monika Petrillová Andrzej Łyskowski Jaroslav Legáth Aleksandra Bocian First Look at the Venom of Naja ashei Molecules Naja ashei venom composition 2-D electrophoresis proteomics |
| title | First Look at the Venom of Naja ashei |
| title_full | First Look at the Venom of Naja ashei |
| title_fullStr | First Look at the Venom of Naja ashei |
| title_full_unstemmed | First Look at the Venom of Naja ashei |
| title_short | First Look at the Venom of Naja ashei |
| title_sort | first look at the venom of naja ashei |
| topic | Naja ashei venom composition 2-D electrophoresis proteomics |
| url | http://www.mdpi.com/1420-3049/23/3/609 |
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