Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol
Phytosterols are important micronutrients that are precursors of important molting hormones and help maintain cellular membrane integrity in insects including bees. Previous research has shown that 24-methylenecholesterol is a key phytosterol that enhances honey bee longevity and improves nurse bee...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/11/743 |
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| author | Priyadarshini Chakrabarti Ramesh R. Sagili |
| author_facet | Priyadarshini Chakrabarti Ramesh R. Sagili |
| author_sort | Priyadarshini Chakrabarti |
| collection | DOAJ |
| description | Phytosterols are important micronutrients that are precursors of important molting hormones and help maintain cellular membrane integrity in insects including bees. Previous research has shown that 24-methylenecholesterol is a key phytosterol that enhances honey bee longevity and improves nurse bee physiology. Nurse bees have the ability to selectively transfer this sterol to developing larvae through brood food. This study examines the physiological impacts of 24-methylenecholesterol on nurse bees, by analyzing the protein profiles of nurse bee heads upon dietary sterol manipulation. Dietary experimental groups consisting of newly emerged honey bees were provided with varying concentrations of 24-methylenecholesterol for three weeks. At the end of the study, honey bees were collected and proteomic analysis was performed on honey bee heads. A total of 1715 proteins were identified across experimental groups. The mean relative abundances of nutritional marker proteins (<i>viz</i>. major royal jelly proteins 1, 4, 5, 7) were higher in experimental groups supplemented with higher dietary sterol concentrations, when compared with the control dietary group. The mean relative abundances of important enzymatic proteins (aminopeptidase and calcium-transporting ATPase) were higher in control groups, whereas mean relative abundances of oxysterol-binding protein and fatty acid-binding protein were higher in higher dietary sterol groups. |
| first_indexed | 2024-03-10T15:14:14Z |
| format | Article |
| id | doaj.art-cac7322521cc49de878fd50967d1a068 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T15:14:14Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-cac7322521cc49de878fd50967d1a0682023-11-20T19:03:35ZengMDPI AGInsects2075-44502020-10-01111174310.3390/insects11110743Changes in Honey Bee Head Proteome in Response to Dietary 24-MethylenecholesterolPriyadarshini Chakrabarti0Ramesh R. Sagili1Department of Horticulture, Oregon State University, Corvallis, OR 97331, USADepartment of Horticulture, Oregon State University, Corvallis, OR 97331, USAPhytosterols are important micronutrients that are precursors of important molting hormones and help maintain cellular membrane integrity in insects including bees. Previous research has shown that 24-methylenecholesterol is a key phytosterol that enhances honey bee longevity and improves nurse bee physiology. Nurse bees have the ability to selectively transfer this sterol to developing larvae through brood food. This study examines the physiological impacts of 24-methylenecholesterol on nurse bees, by analyzing the protein profiles of nurse bee heads upon dietary sterol manipulation. Dietary experimental groups consisting of newly emerged honey bees were provided with varying concentrations of 24-methylenecholesterol for three weeks. At the end of the study, honey bees were collected and proteomic analysis was performed on honey bee heads. A total of 1715 proteins were identified across experimental groups. The mean relative abundances of nutritional marker proteins (<i>viz</i>. major royal jelly proteins 1, 4, 5, 7) were higher in experimental groups supplemented with higher dietary sterol concentrations, when compared with the control dietary group. The mean relative abundances of important enzymatic proteins (aminopeptidase and calcium-transporting ATPase) were higher in control groups, whereas mean relative abundances of oxysterol-binding protein and fatty acid-binding protein were higher in higher dietary sterol groups.https://www.mdpi.com/2075-4450/11/11/743nurse beehoney bee proteomicsphytosterol24-methylenecholesterolhoney bee nutritionhoney bee physiology |
| spellingShingle | Priyadarshini Chakrabarti Ramesh R. Sagili Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol Insects nurse bee honey bee proteomics phytosterol 24-methylenecholesterol honey bee nutrition honey bee physiology |
| title | Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol |
| title_full | Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol |
| title_fullStr | Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol |
| title_full_unstemmed | Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol |
| title_short | Changes in Honey Bee Head Proteome in Response to Dietary 24-Methylenecholesterol |
| title_sort | changes in honey bee head proteome in response to dietary 24 methylenecholesterol |
| topic | nurse bee honey bee proteomics phytosterol 24-methylenecholesterol honey bee nutrition honey bee physiology |
| url | https://www.mdpi.com/2075-4450/11/11/743 |
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