<i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach
A cyanobacterial strain (ZJJ01), morphologically identified as a <i>Leptolyngbya</i>-like species was isolated from a freshwater pond in Zhangjiajie National Forest Park, China, and it was characterized through a polyphasic study based on morphological, ecological, and molecular data. Mo...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/1424-2818/14/5/364 |
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| author | Fangfang Cai Shuheng Li Hang Zhang Gongliang Yu Renhui Li |
| author_facet | Fangfang Cai Shuheng Li Hang Zhang Gongliang Yu Renhui Li |
| author_sort | Fangfang Cai |
| collection | DOAJ |
| description | A cyanobacterial strain (ZJJ01), morphologically identified as a <i>Leptolyngbya</i>-like species was isolated from a freshwater pond in Zhangjiajie National Forest Park, China, and it was characterized through a polyphasic study based on morphological, ecological, and molecular data. Morphologically, the strain fits the description of <i>Leptolyngbya</i> well, but under further examination, it formed a distinctive structure, namely nodules, which confirmed that it belonged to the genus of <i>Nodosilinea</i>. The 16S rRNA gene threshold value and 16S rRNA phylogenetic analyses clearly confirmed that the studied strain belongs to the genus <i>Nodosilinea</i> but that it is phylogenetically distinct from the 10 other species of <i>Nodosilinea</i>. Furthermore, The D1–D1’ and Box–B helix of the 16S–23S ITS region of the strain ZJJ01 were also different from those of previously described <i>Nodosilinea</i> species. On the basis of this polyphasic approach, here, we provide a description of the new taxon: <i>Nodosilinea hunanesis</i> sp. nov. |
| first_indexed | 2024-03-10T03:02:17Z |
| format | Article |
| id | doaj.art-cacb516ce8ce470888a6bccdcbde2eed |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T03:02:17Z |
| publishDate | 2022-05-01 |
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| spelling | doaj.art-cacb516ce8ce470888a6bccdcbde2eed2023-11-23T10:43:07ZengMDPI AGDiversity1424-28182022-05-0114536410.3390/d14050364<i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic ApproachFangfang Cai0Shuheng Li1Hang Zhang2Gongliang Yu3Renhui Li4Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, ChinaHubei Water Resources Research Institute, Hubei Water Resources and Hydropower Science and Technology Information Center, Wuhan 430070, ChinaKey Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaSchool of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, ChinaA cyanobacterial strain (ZJJ01), morphologically identified as a <i>Leptolyngbya</i>-like species was isolated from a freshwater pond in Zhangjiajie National Forest Park, China, and it was characterized through a polyphasic study based on morphological, ecological, and molecular data. Morphologically, the strain fits the description of <i>Leptolyngbya</i> well, but under further examination, it formed a distinctive structure, namely nodules, which confirmed that it belonged to the genus of <i>Nodosilinea</i>. The 16S rRNA gene threshold value and 16S rRNA phylogenetic analyses clearly confirmed that the studied strain belongs to the genus <i>Nodosilinea</i> but that it is phylogenetically distinct from the 10 other species of <i>Nodosilinea</i>. Furthermore, The D1–D1’ and Box–B helix of the 16S–23S ITS region of the strain ZJJ01 were also different from those of previously described <i>Nodosilinea</i> species. On the basis of this polyphasic approach, here, we provide a description of the new taxon: <i>Nodosilinea hunanesis</i> sp. nov.https://www.mdpi.com/1424-2818/14/5/36416S rRNA gene16S–23S ITSpolyphasic approach<i>Nodosilinea</i> |
| spellingShingle | Fangfang Cai Shuheng Li Hang Zhang Gongliang Yu Renhui Li <i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach Diversity 16S rRNA gene 16S–23S ITS polyphasic approach <i>Nodosilinea</i> |
| title | <i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach |
| title_full | <i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach |
| title_fullStr | <i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach |
| title_full_unstemmed | <i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach |
| title_short | <i>Nodosilinea hunanesis</i> sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach |
| title_sort | i nodosilinea hunanesis i sp nov prochlorotrichaceae synechococcales from a freshwater pond in china based on a polyphasic approach |
| topic | 16S rRNA gene 16S–23S ITS polyphasic approach <i>Nodosilinea</i> |
| url | https://www.mdpi.com/1424-2818/14/5/364 |
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