Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts
As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts...
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/11/3874 |
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| author | Ute Nazet Susanne Grässel Jonathan Jantsch Peter Proff Agnes Schröder Christian Kirschneck |
| author_facet | Ute Nazet Susanne Grässel Jonathan Jantsch Peter Proff Agnes Schröder Christian Kirschneck |
| author_sort | Ute Nazet |
| collection | DOAJ |
| description | As events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts. Three distinct models of tensile stretching were applied: static isotropic tensile strain at 0 Hz, 16% tension for 48 h; short-term high-frequency cyclic tension at 1 Hz, 10% tension for 4 h; and dynamic tensile stretching for 48 h, consisting of two blocks of moderate stretching at 0.2 Hz, 2%, advanced stretching at 0.5 Hz, 15%, or a combination of both. General signs of inflammation were present after static isotropic tension, whereas short-term high-frequency cyclic tension showed increased levels of <i>IL-6</i> paired with diminished levels of <i>IL-1β</i>. Reduced inflammatory effects of <i>TNF-α</i>, <i>IL-6</i>, and <i>IL-1β</i> were observed when exposed to advanced stretching. Long-term tensile strain induced extracellular matrix remodelling at the gene and protein levels. While hyaluronan acid synthesis was increased with static tensile strain, dynamic tensile stretching had a reducing effect. Our study revealed that proinflammatory markers were activated by mechanical strain as seen in static isotropic tension and short-term high-frequency tensile strain, whereas long-term exposure induced extracellular matrix remodelling processes. |
| first_indexed | 2024-03-10T19:31:03Z |
| format | Article |
| id | doaj.art-cacbf5f6235340c989108ea04a75a5c4 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:31:03Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cacbf5f6235340c989108ea04a75a5c42023-11-20T02:10:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012111387410.3390/ijms21113874Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial FibroblastsUte Nazet0Susanne Grässel1Jonathan Jantsch2Peter Proff3Agnes Schröder4Christian Kirschneck5Department of Orthodontics, University Medical Centre of Regensburg, D-93053 Regensburg, GermanyDepartment of Orthopaedic Surgery, Experimental Orthopaedics, Centre for Medical Biotechnology, University of Regensburg, D-93053 Regensburg, GermanyInstitute of Clinical Microbiology and Hygiene, University Medical Centre of Regensburg, D-93053 Regensburg, GermanyDepartment of Orthodontics, University Medical Centre of Regensburg, D-93053 Regensburg, GermanyDepartment of Orthodontics, University Medical Centre of Regensburg, D-93053 Regensburg, GermanyDepartment of Orthodontics, University Medical Centre of Regensburg, D-93053 Regensburg, GermanyAs events triggering early osteoarthritis onset can be related to mechanical stress and proinflammatory signaling, we investigated the effect of different mechanical strain protocols on the expression of proinflammatory genes, as well as extracellular matrix remodelling in human synovial fibroblasts. Three distinct models of tensile stretching were applied: static isotropic tensile strain at 0 Hz, 16% tension for 48 h; short-term high-frequency cyclic tension at 1 Hz, 10% tension for 4 h; and dynamic tensile stretching for 48 h, consisting of two blocks of moderate stretching at 0.2 Hz, 2%, advanced stretching at 0.5 Hz, 15%, or a combination of both. General signs of inflammation were present after static isotropic tension, whereas short-term high-frequency cyclic tension showed increased levels of <i>IL-6</i> paired with diminished levels of <i>IL-1β</i>. Reduced inflammatory effects of <i>TNF-α</i>, <i>IL-6</i>, and <i>IL-1β</i> were observed when exposed to advanced stretching. Long-term tensile strain induced extracellular matrix remodelling at the gene and protein levels. While hyaluronan acid synthesis was increased with static tensile strain, dynamic tensile stretching had a reducing effect. Our study revealed that proinflammatory markers were activated by mechanical strain as seen in static isotropic tension and short-term high-frequency tensile strain, whereas long-term exposure induced extracellular matrix remodelling processes.https://www.mdpi.com/1422-0067/21/11/3874osteoarthritissynovial fibroblastsmechanical straininflammation |
| spellingShingle | Ute Nazet Susanne Grässel Jonathan Jantsch Peter Proff Agnes Schröder Christian Kirschneck Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts International Journal of Molecular Sciences osteoarthritis synovial fibroblasts mechanical strain inflammation |
| title | Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts |
| title_full | Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts |
| title_fullStr | Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts |
| title_full_unstemmed | Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts |
| title_short | Early OA Stage Like Response Occurs after Dynamic Stretching of Human Synovial Fibroblasts |
| title_sort | early oa stage like response occurs after dynamic stretching of human synovial fibroblasts |
| topic | osteoarthritis synovial fibroblasts mechanical strain inflammation |
| url | https://www.mdpi.com/1422-0067/21/11/3874 |
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