Autofluorescence in eleocytes of some earthworm species.
Immunocompetent cells of earthworms, coelomocytes, comprise adherent amoebocytes and granular eleocytes (chloragocytes). Both cell populations can be expelled via dorsal pores of adult earthworms by exposure to an electric current (4.5 V) for 1 min. Analysis by phase contrast/fluorescence microscopy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Via Medica
2006-04-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4591 |
| _version_ | 1798044909099286528 |
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| author | Barbara Płytycz A John Morgan Stephen R StĂźrzenbaum Michael O' Reilly Graham P Feeney Justyna Cholewa |
| author_facet | Barbara Płytycz A John Morgan Stephen R StĂźrzenbaum Michael O' Reilly Graham P Feeney Justyna Cholewa |
| author_sort | Barbara Płytycz |
| collection | DOAJ |
| description | Immunocompetent cells of earthworms, coelomocytes, comprise adherent amoebocytes and granular eleocytes (chloragocytes). Both cell populations can be expelled via dorsal pores of adult earthworms by exposure to an electric current (4.5 V) for 1 min. Analysis by phase contrast/fluorescence microscopy and flow cytometry demonstrated that eleocyte population of several species exhibits a strong autofluorescence. A high percentage (11-35%) of autofluorescent eleocytes was recorded in Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion sp. (O. cyaneum, O. tyrtaeum tyrtaeum and O. tyrtaeum lacteum). In contrast, autofluorescent coelomocytes were exceptionally scarce (less than 1%) in representative Aporrectodea sp. (A. caliginosa and A. longa) and Lumbricus sp. (L. castaneus, L. festivus, L. rubellus, L. terrestris). Thus, this paper for the first time describes profound intrinsic fluorescence of eleocytes in some--but not all--earthworm species. The function (if any) and inter-species differences of the autofluorescent coelomocytes still remain elusive. |
| first_indexed | 2024-04-11T23:12:25Z |
| format | Article |
| id | doaj.art-cacd61e61da8449b8b65b4bc3ba6800d |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-04-11T23:12:25Z |
| publishDate | 2006-04-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-cacd61e61da8449b8b65b4bc3ba6800d2022-12-22T03:57:48ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312006-04-01441657110.5603/4591Autofluorescence in eleocytes of some earthworm species.Barbara PłytyczA John MorganStephen R StĂźrzenbaumMichael O' ReillyGraham P FeeneyJustyna CholewaImmunocompetent cells of earthworms, coelomocytes, comprise adherent amoebocytes and granular eleocytes (chloragocytes). Both cell populations can be expelled via dorsal pores of adult earthworms by exposure to an electric current (4.5 V) for 1 min. Analysis by phase contrast/fluorescence microscopy and flow cytometry demonstrated that eleocyte population of several species exhibits a strong autofluorescence. A high percentage (11-35%) of autofluorescent eleocytes was recorded in Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion sp. (O. cyaneum, O. tyrtaeum tyrtaeum and O. tyrtaeum lacteum). In contrast, autofluorescent coelomocytes were exceptionally scarce (less than 1%) in representative Aporrectodea sp. (A. caliginosa and A. longa) and Lumbricus sp. (L. castaneus, L. festivus, L. rubellus, L. terrestris). Thus, this paper for the first time describes profound intrinsic fluorescence of eleocytes in some--but not all--earthworm species. The function (if any) and inter-species differences of the autofluorescent coelomocytes still remain elusive.http://czasopisma.viamedica.pl/fhc/article/view/4591 |
| spellingShingle | Barbara Płytycz A John Morgan Stephen R StĂźrzenbaum Michael O' Reilly Graham P Feeney Justyna Cholewa Autofluorescence in eleocytes of some earthworm species. Folia Histochemica et Cytobiologica |
| title | Autofluorescence in eleocytes of some earthworm species. |
| title_full | Autofluorescence in eleocytes of some earthworm species. |
| title_fullStr | Autofluorescence in eleocytes of some earthworm species. |
| title_full_unstemmed | Autofluorescence in eleocytes of some earthworm species. |
| title_short | Autofluorescence in eleocytes of some earthworm species. |
| title_sort | autofluorescence in eleocytes of some earthworm species |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4591 |
| work_keys_str_mv | AT barbarapłytycz autofluorescenceineleocytesofsomeearthwormspecies AT ajohnmorgan autofluorescenceineleocytesofsomeearthwormspecies AT stephenrstazrzenbaum autofluorescenceineleocytesofsomeearthwormspecies AT michaeloreilly autofluorescenceineleocytesofsomeearthwormspecies AT grahampfeeney autofluorescenceineleocytesofsomeearthwormspecies AT justynacholewa autofluorescenceineleocytesofsomeearthwormspecies |