Influence of climatic conditions on in vitro production of bovine embryos

Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus...

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Main Authors: G.A Pessoa, R.B Navarro, L.P Rauber, K.E Brass, C.A.M Silva, M.I.B Rubin
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2010-12-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013&lng=en&tlng=en
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author G.A Pessoa
R.B Navarro
L.P Rauber
K.E Brass
C.A.M Silva
M.I.B Rubin
author_facet G.A Pessoa
R.B Navarro
L.P Rauber
K.E Brass
C.A.M Silva
M.I.B Rubin
author_sort G.A Pessoa
collection DOAJ
description Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo yield declined, possibly because of reduced availability and growth of native pasture.
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spelling doaj.art-cacecdaf40a447a492fcbc144b062c4e2022-12-22T02:39:10ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622010-12-016261381138710.1590/S0102-09352010000600013S0102-09352010000600013Influence of climatic conditions on in vitro production of bovine embryosG.A Pessoa0R.B Navarro1L.P Rauber2K.E Brass3C.A.M Silva4M.I.B Rubin5Universidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaUniversidade Federal de Santa MariaTemperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo yield declined, possibly because of reduced availability and growth of native pasture.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013&lng=en&tlng=enbovinoovócitosembriõesclimaprodução in vitro
spellingShingle G.A Pessoa
R.B Navarro
L.P Rauber
K.E Brass
C.A.M Silva
M.I.B Rubin
Influence of climatic conditions on in vitro production of bovine embryos
Arquivo Brasileiro de Medicina Veterinária e Zootecnia
bovino
ovócitos
embriões
clima
produção in vitro
title Influence of climatic conditions on in vitro production of bovine embryos
title_full Influence of climatic conditions on in vitro production of bovine embryos
title_fullStr Influence of climatic conditions on in vitro production of bovine embryos
title_full_unstemmed Influence of climatic conditions on in vitro production of bovine embryos
title_short Influence of climatic conditions on in vitro production of bovine embryos
title_sort influence of climatic conditions on in vitro production of bovine embryos
topic bovino
ovócitos
embriões
clima
produção in vitro
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013&lng=en&tlng=en
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AT rbnavarro influenceofclimaticconditionsoninvitroproductionofbovineembryos
AT lprauber influenceofclimaticconditionsoninvitroproductionofbovineembryos
AT kebrass influenceofclimaticconditionsoninvitroproductionofbovineembryos
AT camsilva influenceofclimaticconditionsoninvitroproductionofbovineembryos
AT mibrubin influenceofclimaticconditionsoninvitroproductionofbovineembryos