Evaluation of FASTPlaqueTB Test for the Rapid Diagnosis of Mycobacterium tuberculosis Infections
Acid fast bacilli (AFB) smear and culturing the organism are conventional methods for the diagnosis of mycobacterial infections. FASTPlaqueTB is a rapid test that utilises bacteriophage amplification technology for the detection of viable Mycobacterium tuberculosis in clinical specimens. This test c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bilimsel Tip Yayinevi
2002-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2002-7-2-089-093.pdf |
| Summary: | Acid fast bacilli (AFB) smear and culturing the organism are conventional methods for the diagnosis of mycobacterial infections. FASTPlaqueTB is a rapid test that utilises bacteriophage amplification technology for the detection of viable Mycobacterium tuberculosis in clinical specimens. This test can detect 100-300 bacilli/mL and the results are available in 24 hours. We evaluated the performance of the FASTPlaqueTB test by comparing with BACTEC 460 TB culture system, polymerase chain reaction (PCR) and AFB smear methods. We investigated 58 sputum specimens sent to our laboratory. Seventeen AFB smear positive specimens were also positive by FASTPlaqueTB test, BACTEC 460 TB culture system and PCR method. Twenty two of 41 specimens that were negative by microscopy were positive by BACTEC 460 TB culture system and 18 of 41 specimens were found as positive by FASTPlaqueTB test and 17 of 41 specimens were positive by PCR. One specimen was evaluated as positive only by PCR whereas one specimen was positive only by FASTPlaqueTB test. One of the specimens was found as positive by BACTEC 460 TB culture system and FASTPlaqueTB test. The sensitivities of AFB smear, PCR and FASTPlaqueTB test were 43.6%, 84.6% and 87.2% respectively when we accepted BACTEC 460 TB culture system as gold standard. As a result of the evaluation of these three tests according to BACTEC 460 TB culture system we conclude that FASTPlaqueTB test has a good potential for rapid diagnosis of Mycobacterium tuberculosis infections. |
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| ISSN: | 1300-932X 1300-932X |