A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients
Microparticles are sub-micron, membrane-bound particles released from virtually all cells and which are present in the circulation. In several autoimmune disorders their amount and composition in the circulation is altered. Microparticle surface protein expression has been explored as a differentiat...
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9228 |
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| author | Shuaishuai Hu Brenton L. Cavanagh Robert Harrington Muddassar Ahmad Grainne Kearns Steve Meaney Claire Wynne |
| author_facet | Shuaishuai Hu Brenton L. Cavanagh Robert Harrington Muddassar Ahmad Grainne Kearns Steve Meaney Claire Wynne |
| author_sort | Shuaishuai Hu |
| collection | DOAJ |
| description | Microparticles are sub-micron, membrane-bound particles released from virtually all cells and which are present in the circulation. In several autoimmune disorders their amount and composition in the circulation is altered. Microparticle surface protein expression has been explored as a differentiating tool in autoimmune disorders where the clinical pictures can overlap. Here, we examine the utility of a novel lipid-based marker—microparticle cholesterol, present in all microparticles regardless of cellular origin—to distinguish between rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). We first isolated a series of microparticle containing lipoprotein deficient fractions from patient and control plasma. There were no significant differences in the size, structure or protein content of microparticles isolated from each group. Compared to controls, both patient groups contained significantly greater amounts of platelet and endothelial cell-derived microparticles. The cholesterol content of microparticle fractions isolated from RA patients was significantly greater than those from either SLE patients or healthy controls. Our data indicate that circulating non-lipoprotein microparticle cholesterol, which may account for 1–2% of measured cholesterol in patient samples, may represent a novel differentiator of disease, which is independent of cellular origin. |
| first_indexed | 2024-03-10T14:20:46Z |
| format | Article |
| id | doaj.art-cad0a976619845d8895a5e49ff3d72e1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:20:46Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cad0a976619845d8895a5e49ff3d72e12023-11-20T23:24:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012123922810.3390/ijms21239228A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus PatientsShuaishuai Hu0Brenton L. Cavanagh1Robert Harrington2Muddassar Ahmad3Grainne Kearns4Steve Meaney5Claire Wynne6School of Biological and Health Sciences, College of Sciences and Health, Technological University Dublin—City Campus, D08 NF82 Dublin 8, IrelandCellular and Molecular Imaging Core, Royal College of Surgeons in Ireland, D02 YN77 Dublin 2, IrelandDepartment of Rheumatology, Beaumont Hospital, D09 V2N0 Dublin 9, IrelandDepartment of Rheumatology, Beaumont Hospital, D09 V2N0 Dublin 9, IrelandDepartment of Rheumatology, Beaumont Hospital, D09 V2N0 Dublin 9, IrelandSchool of Biological and Health Sciences, College of Sciences and Health, Technological University Dublin—City Campus, D08 NF82 Dublin 8, IrelandSchool of Biological and Health Sciences, College of Sciences and Health, Technological University Dublin—City Campus, D08 NF82 Dublin 8, IrelandMicroparticles are sub-micron, membrane-bound particles released from virtually all cells and which are present in the circulation. In several autoimmune disorders their amount and composition in the circulation is altered. Microparticle surface protein expression has been explored as a differentiating tool in autoimmune disorders where the clinical pictures can overlap. Here, we examine the utility of a novel lipid-based marker—microparticle cholesterol, present in all microparticles regardless of cellular origin—to distinguish between rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). We first isolated a series of microparticle containing lipoprotein deficient fractions from patient and control plasma. There were no significant differences in the size, structure or protein content of microparticles isolated from each group. Compared to controls, both patient groups contained significantly greater amounts of platelet and endothelial cell-derived microparticles. The cholesterol content of microparticle fractions isolated from RA patients was significantly greater than those from either SLE patients or healthy controls. Our data indicate that circulating non-lipoprotein microparticle cholesterol, which may account for 1–2% of measured cholesterol in patient samples, may represent a novel differentiator of disease, which is independent of cellular origin.https://www.mdpi.com/1422-0067/21/23/9228microparticlescholesterolsystemic lupus erythematosusrheumatoid arthritisbiomarker |
| spellingShingle | Shuaishuai Hu Brenton L. Cavanagh Robert Harrington Muddassar Ahmad Grainne Kearns Steve Meaney Claire Wynne A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients International Journal of Molecular Sciences microparticles cholesterol systemic lupus erythematosus rheumatoid arthritis biomarker |
| title | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients |
| title_full | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients |
| title_fullStr | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients |
| title_full_unstemmed | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients |
| title_short | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients |
| title_sort | novel pool of microparticle cholesterol is elevated in rheumatoid arthritis but not in systemic lupus erythematosus patients |
| topic | microparticles cholesterol systemic lupus erythematosus rheumatoid arthritis biomarker |
| url | https://www.mdpi.com/1422-0067/21/23/9228 |
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