L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes
The ability to modulate the catalytic activity of inorganic nanozymes is of high interest. In particular, understanding the interactions of inhibitor molecules with nanozymes can bring them one step closer to the natural enzymes and has thus started to attract intense interest. To date, a few revers...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/5/1285 |
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| author | Piyumi Dinusha Liyanage Pabudi Weerathunge Mandeep Singh Vipul Bansal Rajesh Ramanathan |
| author_facet | Piyumi Dinusha Liyanage Pabudi Weerathunge Mandeep Singh Vipul Bansal Rajesh Ramanathan |
| author_sort | Piyumi Dinusha Liyanage |
| collection | DOAJ |
| description | The ability to modulate the catalytic activity of inorganic nanozymes is of high interest. In particular, understanding the interactions of inhibitor molecules with nanozymes can bring them one step closer to the natural enzymes and has thus started to attract intense interest. To date, a few reversible inhibitors of the nanozyme activity have been reported. However, there are no reports of irreversible inhibitor molecules that can permanently inhibit the activity of nanozymes. In the current work, we show the ability of L-cysteine to act as an irreversible inhibitor to permanently block the nanozyme activity of 2-dimensional (2D) NiO nanosheets. Determination of the steady state kinetic parameters allowed us to obtain mechanistic insights into the catalytic inhibition process. Further, based on the irreversible catalytic inhibition capability of L-cysteine, we demonstrate a highly specific sensor for the detection of this biologically important molecule. |
| first_indexed | 2024-03-10T11:27:11Z |
| format | Article |
| id | doaj.art-cad2a9a67d564ab2a5b072d1b571597c |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T11:27:11Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-cad2a9a67d564ab2a5b072d1b571597c2023-11-21T19:35:33ZengMDPI AGNanomaterials2079-49912021-05-01115128510.3390/nano11051285L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based NanozymesPiyumi Dinusha Liyanage0Pabudi Weerathunge1Mandeep Singh2Vipul Bansal3Rajesh Ramanathan4Ian Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC 3000, AustraliaIan Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC 3000, AustraliaIan Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC 3000, AustraliaIan Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC 3000, AustraliaIan Potter NanoBioSensing Facility, NanoBiotechnology Research Laboratory (NBRL), School of Science, RMIT University, GPO Box 2476, Melbourne, VIC 3000, AustraliaThe ability to modulate the catalytic activity of inorganic nanozymes is of high interest. In particular, understanding the interactions of inhibitor molecules with nanozymes can bring them one step closer to the natural enzymes and has thus started to attract intense interest. To date, a few reversible inhibitors of the nanozyme activity have been reported. However, there are no reports of irreversible inhibitor molecules that can permanently inhibit the activity of nanozymes. In the current work, we show the ability of L-cysteine to act as an irreversible inhibitor to permanently block the nanozyme activity of 2-dimensional (2D) NiO nanosheets. Determination of the steady state kinetic parameters allowed us to obtain mechanistic insights into the catalytic inhibition process. Further, based on the irreversible catalytic inhibition capability of L-cysteine, we demonstrate a highly specific sensor for the detection of this biologically important molecule.https://www.mdpi.com/2079-4991/11/5/1285nanozymeL-cysteineinhibitorirreversible inhibitorenzyme-mimicperoxidase-mimic |
| spellingShingle | Piyumi Dinusha Liyanage Pabudi Weerathunge Mandeep Singh Vipul Bansal Rajesh Ramanathan L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes Nanomaterials nanozyme L-cysteine inhibitor irreversible inhibitor enzyme-mimic peroxidase-mimic |
| title | L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes |
| title_full | L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes |
| title_fullStr | L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes |
| title_full_unstemmed | L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes |
| title_short | L-Cysteine as an Irreversible Inhibitor of the Peroxidase-Mimic Catalytic Activity of 2-Dimensional Ni-Based Nanozymes |
| title_sort | l cysteine as an irreversible inhibitor of the peroxidase mimic catalytic activity of 2 dimensional ni based nanozymes |
| topic | nanozyme L-cysteine inhibitor irreversible inhibitor enzyme-mimic peroxidase-mimic |
| url | https://www.mdpi.com/2079-4991/11/5/1285 |
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