The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress
Lysine malonylation (Kmal) is an evolutionarily conserved post-translational modification (PTM) that has been demonstrated to be involved in cellular and organismal metabolism. However, the role that Kmal plays in response to drought stress of the terrestrial cyanobacteria <i>N. flagelliforme&...
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MDPI AG
2023-05-01
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| author | Meng Wang Qiang Zhu Ning Yao Wangli Liang Xiaoxia Ma Jingjing Li Xiaoxu Li Lingxia Wang Wenyu Liang |
| author_facet | Meng Wang Qiang Zhu Ning Yao Wangli Liang Xiaoxia Ma Jingjing Li Xiaoxu Li Lingxia Wang Wenyu Liang |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | Lysine malonylation (Kmal) is an evolutionarily conserved post-translational modification (PTM) that has been demonstrated to be involved in cellular and organismal metabolism. However, the role that Kmal plays in response to drought stress of the terrestrial cyanobacteria <i>N. flagelliforme</i> is still unknown. In this study, we performed the first proteomic analysis of Kmal in <i>N. flagelliforme</i> under different drought stresses using LC-MS/MS. In total, 421 malonylated lysine residues were found in 236 different proteins. GO and KEGG enrichment analysis indicated that these malonylated proteins were highly enriched in several metabolic pathways, including carbon metabolism and photosynthesis. Decreased malonylation levels were found to hinder the reception and transmission of light energy and CO<sub>2</sub> fixation, which led to a decrease in photosynthetic activity. Kmal was also shown to inhibit the flux of the TCA cycle and activate the gluconeogenesis pathway in response to drought stress. Furthermore, malonylated antioxidant enzymes and antioxidants were synergistically involved in reactive oxygen species (ROS) scavenging. Malonylation was involved in lipid degradation and amino acid biosynthesis as part of drought stress adaptation. This work represents the first comprehensive investigation of the role of malonylation in dehydrated <i>N. flagelliforme</i>, providing an important resource for understanding the drought tolerance mechanism of this organism. |
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| issn | 1661-6596 1422-0067 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cae5c5dcd3bf4a86868817615e82b0b92023-11-17T23:09:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249844610.3390/ijms24098446The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought StressMeng Wang0Qiang Zhu1Ning Yao2Wangli Liang3Xiaoxia Ma4Jingjing Li5Xiaoxu Li6Lingxia Wang7Wenyu Liang8School of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaSchool of Life Sciences, Ningxia University, Yinchuan 750021, ChinaLysine malonylation (Kmal) is an evolutionarily conserved post-translational modification (PTM) that has been demonstrated to be involved in cellular and organismal metabolism. However, the role that Kmal plays in response to drought stress of the terrestrial cyanobacteria <i>N. flagelliforme</i> is still unknown. In this study, we performed the first proteomic analysis of Kmal in <i>N. flagelliforme</i> under different drought stresses using LC-MS/MS. In total, 421 malonylated lysine residues were found in 236 different proteins. GO and KEGG enrichment analysis indicated that these malonylated proteins were highly enriched in several metabolic pathways, including carbon metabolism and photosynthesis. Decreased malonylation levels were found to hinder the reception and transmission of light energy and CO<sub>2</sub> fixation, which led to a decrease in photosynthetic activity. Kmal was also shown to inhibit the flux of the TCA cycle and activate the gluconeogenesis pathway in response to drought stress. Furthermore, malonylated antioxidant enzymes and antioxidants were synergistically involved in reactive oxygen species (ROS) scavenging. Malonylation was involved in lipid degradation and amino acid biosynthesis as part of drought stress adaptation. This work represents the first comprehensive investigation of the role of malonylation in dehydrated <i>N. flagelliforme</i>, providing an important resource for understanding the drought tolerance mechanism of this organism.https://www.mdpi.com/1422-0067/24/9/8446terrestrial cyanobacterialysine malonylationdrought stresscarbon metabolismphotosynthesismolecular function |
| spellingShingle | Meng Wang Qiang Zhu Ning Yao Wangli Liang Xiaoxia Ma Jingjing Li Xiaoxu Li Lingxia Wang Wenyu Liang The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress International Journal of Molecular Sciences terrestrial cyanobacteria lysine malonylation drought stress carbon metabolism photosynthesis molecular function |
| title | The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress |
| title_full | The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress |
| title_fullStr | The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress |
| title_full_unstemmed | The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress |
| title_short | The Enzyme Lysine Malonylation of Calvin Cycle and Gluconeogenesis Regulated Glycometabolism in <i>Nostoc flagelliforme</i> to Adapt to Drought Stress |
| title_sort | enzyme lysine malonylation of calvin cycle and gluconeogenesis regulated glycometabolism in i nostoc flagelliforme i to adapt to drought stress |
| topic | terrestrial cyanobacteria lysine malonylation drought stress carbon metabolism photosynthesis molecular function |
| url | https://www.mdpi.com/1422-0067/24/9/8446 |
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