Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1

Background: Sirtuins mediate metabolic responses to nutrient availability and slow aging and accompanying decline in health. This study was designed to assess the expressions of sirtuin1 (SIRT1) and sirtuin3 (SIRT3) in the liver and hypothalamus after duodenal-jejunal bypass (DJB) surgery in rats. M...

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Main Authors: Eunyoung Ha, Jong Yeon Kang, Kyung Sik Park, Youn Kyoung Seo, Tae Kyung Ha
Format: Article
Language:English
Published: Korean Society for the Study of Obesity 2018-12-01
Series:Journal of Obesity & Metabolic Syndrome
Subjects:
Online Access:https://doi.org/10.7570/jomes.2018.27.4.248
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author Eunyoung Ha
Jong Yeon Kang
Kyung Sik Park
Youn Kyoung Seo
Tae Kyung Ha
author_facet Eunyoung Ha
Jong Yeon Kang
Kyung Sik Park
Youn Kyoung Seo
Tae Kyung Ha
author_sort Eunyoung Ha
collection DOAJ
description Background: Sirtuins mediate metabolic responses to nutrient availability and slow aging and accompanying decline in health. This study was designed to assess the expressions of sirtuin1 (SIRT1) and sirtuin3 (SIRT3) in the liver and hypothalamus after duodenal-jejunal bypass (DJB) surgery in rats. Methods: A total of 38 rats were randomly assigned to either sham group (n=8) or DJB group (n=30). DJB group was again divided into three groups according to the elapsed time after surgery (10 weeks, DJB10; 16 week, DJB16; 28 week, DJB28). The mRNA and protein expressions of SIRT1 and SIRT3 in the liver and hypothalamus were measured by reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry analyses. NAD⁺/NADH ratio was also measured. Results: We found increased mRNA and protein expression levels of SIRT1 in the liver of DJB16 and DJB28 groups compared with those of sham group. The mRNA and protein expressions of SIRT3 in the liver of DJB group increased proportionally to the elapsed time after DJB surgery. The mRNA expression levels of SIRT1 in the hypothalamus increased in DJB16 and DJB28 groups and protein expression levels of SIRT1 in the hypothalamus increased in DJB10, DBJ16, and DJB28 groups compared with sham group. We observed that mRNA and protein levels of SIRT3 in the hypothalamus of DJB group were not changed. Conclusion: This study proves that DJB increases SIRT1 and SIRT3 expressions in the liver and SIRT1 expression in the hypothalamus. These results suggest the possibility of sirtuins being involved in bypass surgery-induced metabolic changes.
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spelling doaj.art-cae674ffc06d4317ac76faf85b478ffa2022-12-21T18:41:13ZengKorean Society for the Study of ObesityJournal of Obesity & Metabolic Syndrome2508-62352018-12-0127424825310.7570/jomes.2018.27.4.248jomes.2018.27.4.248Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1Eunyoung Ha0Jong Yeon Kang1Kyung Sik Park2Youn Kyoung Seo3Tae Kyung Ha4Department of Biochemistry, Keimyung University School of Medicine, Daegu, KoreaDepartment of Biochemistry, Keimyung University School of Medicine, Daegu, KoreaDepartment of Biochemistry, Keimyung University School of Medicine, Daegu, KoreaDepartment of Anatomy and Cell Biology, Hanyang University College of Medicine, Seoul, KoreaDepartment of Surgery, Hanyang University College of Medicine, Seoul, KoreaBackground: Sirtuins mediate metabolic responses to nutrient availability and slow aging and accompanying decline in health. This study was designed to assess the expressions of sirtuin1 (SIRT1) and sirtuin3 (SIRT3) in the liver and hypothalamus after duodenal-jejunal bypass (DJB) surgery in rats. Methods: A total of 38 rats were randomly assigned to either sham group (n=8) or DJB group (n=30). DJB group was again divided into three groups according to the elapsed time after surgery (10 weeks, DJB10; 16 week, DJB16; 28 week, DJB28). The mRNA and protein expressions of SIRT1 and SIRT3 in the liver and hypothalamus were measured by reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry analyses. NAD⁺/NADH ratio was also measured. Results: We found increased mRNA and protein expression levels of SIRT1 in the liver of DJB16 and DJB28 groups compared with those of sham group. The mRNA and protein expressions of SIRT3 in the liver of DJB group increased proportionally to the elapsed time after DJB surgery. The mRNA expression levels of SIRT1 in the hypothalamus increased in DJB16 and DJB28 groups and protein expression levels of SIRT1 in the hypothalamus increased in DJB10, DBJ16, and DJB28 groups compared with sham group. We observed that mRNA and protein levels of SIRT3 in the hypothalamus of DJB group were not changed. Conclusion: This study proves that DJB increases SIRT1 and SIRT3 expressions in the liver and SIRT1 expression in the hypothalamus. These results suggest the possibility of sirtuins being involved in bypass surgery-induced metabolic changes.https://doi.org/10.7570/jomes.2018.27.4.248Duodenojejunal bypassLife spanSirtuin1Sirtuin3
spellingShingle Eunyoung Ha
Jong Yeon Kang
Kyung Sik Park
Youn Kyoung Seo
Tae Kyung Ha
Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1
Journal of Obesity & Metabolic Syndrome
Duodenojejunal bypass
Life span
Sirtuin1
Sirtuin3
title Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1
title_full Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1
title_fullStr Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1
title_full_unstemmed Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1
title_short Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1
title_sort duodenal jejunal bypass surgery stimulates the expressions of hepatic sirtuin1 and 3 and hypothalamic sirtuin1
topic Duodenojejunal bypass
Life span
Sirtuin1
Sirtuin3
url https://doi.org/10.7570/jomes.2018.27.4.248
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