The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis
The ETHYLENE INSENSITIVE3‐LIKE (EIL) transcription factor family plays a critical role in the ethylene signaling pathway, which regulates a broad spectrum of plant growth and developmental processes, as well as defenses to myriad stresses. Although genome‐wide analysis of this family has been carrie...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-04-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12596 |
| _version_ | 1797821942436200448 |
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| author | Qing Li Yanting Shen Luqin Guo Hong Wang Yu Zhang Chengming Fan Yihong Zheng |
| author_facet | Qing Li Yanting Shen Luqin Guo Hong Wang Yu Zhang Chengming Fan Yihong Zheng |
| author_sort | Qing Li |
| collection | DOAJ |
| description | The ETHYLENE INSENSITIVE3‐LIKE (EIL) transcription factor family plays a critical role in the ethylene signaling pathway, which regulates a broad spectrum of plant growth and developmental processes, as well as defenses to myriad stresses. Although genome‐wide analysis of this family has been carried out for several plant species, no comprehensive analysis of the EIL gene family in soybean has been reported so far. Furthermore, there are few studies on the functions of EIL genes in soybean. In this study, we identified 12 soybean (Gm) EIL genes, which we divided into three groups based on their phylogenetic relationships. We then detected their duplication status and found that most of the GmEIL genes have duplicated copies derived from two whole‐genome duplication events. These duplicated genes underwent strong negative selection during evolution. We further analyzed the transcript profiles of GmEIL genes using the transcriptome data and found that their spatio‐temporal and stress expression patterns varied considerably. For example, GmEIL1–GmEIL5 were found to be strongly expressed in almost every sample, while GmEIL8–GmEIL12 exhibited low expression, or were not expressed at all. Additionally, these genes showed different responses to dehydration, salinity and phosphate starvation. Finally, we surveyed genetic variations of these genes in 302 resequenced wild soybeans, landraces and improved soybean cultivars. Our data showed that most GmEIL genes are well conserved, and are not modified in domesticated or improved cultivars. Together, these findings provide a potentially valuable resource for characterizing the GmEIL gene family and lay the basis for further elucidation of their molecular mechanisms. |
| first_indexed | 2024-03-13T10:00:09Z |
| format | Article |
| id | doaj.art-cae71407d8be463698279f00b59b4f2c |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-13T10:00:09Z |
| publishDate | 2019-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-cae71407d8be463698279f00b59b4f2c2023-05-23T06:46:21ZengWileyFEBS Open Bio2211-54632019-04-019462964210.1002/2211-5463.12596The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysisQing Li0Yanting Shen1Luqin Guo2Hong Wang3Yu Zhang4Chengming Fan5Yihong Zheng6College of Life Sciences and Oceanography Shenzhen University ChinaInstitute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing ChinaCollege of Horticulture Henan Agricultural University Zhengzhou ChinaCollege of Life Sciences and Oceanography Shenzhen University ChinaCollege of Life Sciences and Oceanography Shenzhen University ChinaInstitute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing ChinaCollege of Life Sciences and Oceanography Shenzhen University ChinaThe ETHYLENE INSENSITIVE3‐LIKE (EIL) transcription factor family plays a critical role in the ethylene signaling pathway, which regulates a broad spectrum of plant growth and developmental processes, as well as defenses to myriad stresses. Although genome‐wide analysis of this family has been carried out for several plant species, no comprehensive analysis of the EIL gene family in soybean has been reported so far. Furthermore, there are few studies on the functions of EIL genes in soybean. In this study, we identified 12 soybean (Gm) EIL genes, which we divided into three groups based on their phylogenetic relationships. We then detected their duplication status and found that most of the GmEIL genes have duplicated copies derived from two whole‐genome duplication events. These duplicated genes underwent strong negative selection during evolution. We further analyzed the transcript profiles of GmEIL genes using the transcriptome data and found that their spatio‐temporal and stress expression patterns varied considerably. For example, GmEIL1–GmEIL5 were found to be strongly expressed in almost every sample, while GmEIL8–GmEIL12 exhibited low expression, or were not expressed at all. Additionally, these genes showed different responses to dehydration, salinity and phosphate starvation. Finally, we surveyed genetic variations of these genes in 302 resequenced wild soybeans, landraces and improved soybean cultivars. Our data showed that most GmEIL genes are well conserved, and are not modified in domesticated or improved cultivars. Together, these findings provide a potentially valuable resource for characterizing the GmEIL gene family and lay the basis for further elucidation of their molecular mechanisms.https://doi.org/10.1002/2211-5463.12596EIL gene familyethyleneethylene signalingsoybean |
| spellingShingle | Qing Li Yanting Shen Luqin Guo Hong Wang Yu Zhang Chengming Fan Yihong Zheng The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis FEBS Open Bio EIL gene family ethylene ethylene signaling soybean |
| title | The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis |
| title_full | The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis |
| title_fullStr | The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis |
| title_full_unstemmed | The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis |
| title_short | The EIL transcription factor family in soybean: Genome‐wide identification, expression profiling and genetic diversity analysis |
| title_sort | eil transcription factor family in soybean genome wide identification expression profiling and genetic diversity analysis |
| topic | EIL gene family ethylene ethylene signaling soybean |
| url | https://doi.org/10.1002/2211-5463.12596 |
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