Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis
Enteric adenoviruses (EAd), which are the major causative agents of acute gastroenteritis next to rotavirus in young children, are classified among the endemic group of adenoviruses (Adv) today. In this study, 194 fecal samples obtained from children with acute gastroenteritis admitted to Hacettepe...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
1997-09-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1997-2-3-195-207.pdf |
| _version_ | 1797914975354748928 |
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| author | Serdar TUNCER Mehmet CEYHAN Kadriye YURDAKÖK Güler KANRA Şemsettin USTAÇELEBİ |
| author_facet | Serdar TUNCER Mehmet CEYHAN Kadriye YURDAKÖK Güler KANRA Şemsettin USTAÇELEBİ |
| author_sort | Serdar TUNCER |
| collection | DOAJ |
| description | Enteric adenoviruses (EAd), which are the major causative agents of acute gastroenteritis next to rotavirus in young children, are classified among the endemic group of adenoviruses (Adv) today. In this study, 194 fecal samples obtained from children with acute gastroenteritis admitted to Hacettepe Children Hospital Diarrhea Center were analyzed for the presence of EAd during the period August 1993 to May 1995. Fecal specimens were tested by ELISA with Adv group, type 40 and 41 specific monoclonal antibodies. All fecal specimens were also inoculated into KB and Graham 293 cells. Viral isolates were typed by restriction endonuclease analysis. Adv 40 and Adv 41 were detected 6 (3.1%) and 4 (2.1%) of 15 isolates from all fecal specimens, respectively. ELISA and isolation results have shown high correlation for the detection of Adv and EAd. Of children infected with EAd 90% were under 2 years old. Seasonal EAd isolation rates were not significantly different. These results have shown that Adv 40 and 41 are also important causes of acute viral gastroenteritis in our population. Antigenic and genotypic variants were not detected in enteric adenoviral isolates. |
| first_indexed | 2024-04-10T12:34:30Z |
| format | Article |
| id | doaj.art-cae8bbe655b243e4b7d770cd718489a2 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T12:34:30Z |
| publishDate | 1997-09-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-cae8bbe655b243e4b7d770cd718489a22023-02-15T16:14:44ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X1997-09-0123195207Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with GastroenteritisSerdar TUNCER0Mehmet CEYHAN1Kadriye YURDAKÖK2Güler KANRA3Şemsettin USTAÇELEBİ4Hacettepe Üniversitesi Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İnfeksiyon Hastalıkları Ünitesi,Hacettepe Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, İnfeksiyon Hastalıkları Bilim Dalı,Hacettepe Üniversitesi Tıp Fakültesi, Çocuk Sağlığı Enstitüsü, Sosyal Pediatri Anabilim Dalı,Hacettepe Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, İnfeksiyon Hastalıkları Bilim Dalı,Haccettepe Üniversitesi Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, ANKARAEnteric adenoviruses (EAd), which are the major causative agents of acute gastroenteritis next to rotavirus in young children, are classified among the endemic group of adenoviruses (Adv) today. In this study, 194 fecal samples obtained from children with acute gastroenteritis admitted to Hacettepe Children Hospital Diarrhea Center were analyzed for the presence of EAd during the period August 1993 to May 1995. Fecal specimens were tested by ELISA with Adv group, type 40 and 41 specific monoclonal antibodies. All fecal specimens were also inoculated into KB and Graham 293 cells. Viral isolates were typed by restriction endonuclease analysis. Adv 40 and Adv 41 were detected 6 (3.1%) and 4 (2.1%) of 15 isolates from all fecal specimens, respectively. ELISA and isolation results have shown high correlation for the detection of Adv and EAd. Of children infected with EAd 90% were under 2 years old. Seasonal EAd isolation rates were not significantly different. These results have shown that Adv 40 and 41 are also important causes of acute viral gastroenteritis in our population. Antigenic and genotypic variants were not detected in enteric adenoviral isolates.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1997-2-3-195-207.pdfEnteric AdenovirusesELISARestriction Endonuclease Analysis |
| spellingShingle | Serdar TUNCER Mehmet CEYHAN Kadriye YURDAKÖK Güler KANRA Şemsettin USTAÇELEBİ Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi Enteric Adenoviruses ELISA Restriction Endonuclease Analysis |
| title | Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis |
| title_full | Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis |
| title_fullStr | Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis |
| title_full_unstemmed | Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis |
| title_short | Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric Adenovirus Infection Rate in Children with Gastroenteritis |
| title_sort | typing of human adenoviruses by restriction endonuclease analysis and enteric adenovirus infection rate in children with gastroenteritis |
| topic | Enteric Adenoviruses ELISA Restriction Endonuclease Analysis |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1997-2-3-195-207.pdf |
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