Intraoperative radiation therapy induces immune response activity after pancreatic surgery
Abstract Background Pancreatic cancer has highly aggressive features, such as local recurrence that leads to significantly high morbidity and mortality and recurrence after successful tumour resection. Intraoperative radiation therapy (IORT), which delivers targeted radiation to a tumour bed, is kno...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-021-08807-3 |
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| author | Yun Sun Lee Hyung Sun Kim Yeona Cho Ik Jae Lee Hyo Jung Kim Da Eun Lee Hyeon Woong Kang Joon Seong Park |
| author_facet | Yun Sun Lee Hyung Sun Kim Yeona Cho Ik Jae Lee Hyo Jung Kim Da Eun Lee Hyeon Woong Kang Joon Seong Park |
| author_sort | Yun Sun Lee |
| collection | DOAJ |
| description | Abstract Background Pancreatic cancer has highly aggressive features, such as local recurrence that leads to significantly high morbidity and mortality and recurrence after successful tumour resection. Intraoperative radiation therapy (IORT), which delivers targeted radiation to a tumour bed, is known to reduce local recurrence by directly killing tumour cells and modifying the tumour microenvironment. Methods Among 30 patients diagnosed with pancreatic cancer, 17 patients received IORT immediately after surgical resection. We investigated changes in the immune response induced by IORT by analysing the peritoneal fluid (PF) and blood of patients with and without IORT treatment after pancreatic cancer surgery. Further, we treated three pancreatic cell lines with PF to observe proliferation and activity changes. Results Levels of cytokines involved in the PI3K/SMAD pathway were increased in the PF of IORT-treated patients. Moreover, IORT-treated PF inhibited the growth, migration, and invasiveness of pancreatic cancer cells. Changes in lymphocyte populations in the blood of IORT-treated patients indicated an increased immune response. Conclusions Based on the characterisation and quantification of immune cells in the blood and cytokine levels in the PF, we conclude that IORT induced an anti-tumour effect by activating the immune response, which may prevent pancreatic cancer recurrence. Clinical trial registration NCT03273374 . |
| first_indexed | 2024-12-17T21:30:56Z |
| format | Article |
| id | doaj.art-cae8fcd38ce642ad91f81a9de8d373db |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-17T21:30:56Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-cae8fcd38ce642ad91f81a9de8d373db2022-12-21T21:31:53ZengBMCBMC Cancer1471-24072021-10-0121111210.1186/s12885-021-08807-3Intraoperative radiation therapy induces immune response activity after pancreatic surgeryYun Sun Lee0Hyung Sun Kim1Yeona Cho2Ik Jae Lee3Hyo Jung Kim4Da Eun Lee5Hyeon Woong Kang6Joon Seong Park7Department of Surgery, Gangnam Severance Hospital, Yonsei University College of MedicineDepartment of Surgery, Gangnam Severance Hospital, Yonsei University College of MedicineDepartment of Radiation Oncology, Yonsei University College of MedicineDepartment of Radiation Oncology, Yonsei University College of MedicineDepartment of Surgery, Gangnam Severance Hospital, Yonsei University College of MedicineDepartment of Surgery, Gangnam Severance Hospital, Yonsei University College of MedicineDepartment of Surgery, Gangnam Severance Hospital, Yonsei University College of MedicineDepartment of Surgery, Gangnam Severance Hospital, Yonsei University College of MedicineAbstract Background Pancreatic cancer has highly aggressive features, such as local recurrence that leads to significantly high morbidity and mortality and recurrence after successful tumour resection. Intraoperative radiation therapy (IORT), which delivers targeted radiation to a tumour bed, is known to reduce local recurrence by directly killing tumour cells and modifying the tumour microenvironment. Methods Among 30 patients diagnosed with pancreatic cancer, 17 patients received IORT immediately after surgical resection. We investigated changes in the immune response induced by IORT by analysing the peritoneal fluid (PF) and blood of patients with and without IORT treatment after pancreatic cancer surgery. Further, we treated three pancreatic cell lines with PF to observe proliferation and activity changes. Results Levels of cytokines involved in the PI3K/SMAD pathway were increased in the PF of IORT-treated patients. Moreover, IORT-treated PF inhibited the growth, migration, and invasiveness of pancreatic cancer cells. Changes in lymphocyte populations in the blood of IORT-treated patients indicated an increased immune response. Conclusions Based on the characterisation and quantification of immune cells in the blood and cytokine levels in the PF, we conclude that IORT induced an anti-tumour effect by activating the immune response, which may prevent pancreatic cancer recurrence. Clinical trial registration NCT03273374 .https://doi.org/10.1186/s12885-021-08807-3Pancreatic cancerIntraoperative radiation therapyImmune responseCytokine |
| spellingShingle | Yun Sun Lee Hyung Sun Kim Yeona Cho Ik Jae Lee Hyo Jung Kim Da Eun Lee Hyeon Woong Kang Joon Seong Park Intraoperative radiation therapy induces immune response activity after pancreatic surgery BMC Cancer Pancreatic cancer Intraoperative radiation therapy Immune response Cytokine |
| title | Intraoperative radiation therapy induces immune response activity after pancreatic surgery |
| title_full | Intraoperative radiation therapy induces immune response activity after pancreatic surgery |
| title_fullStr | Intraoperative radiation therapy induces immune response activity after pancreatic surgery |
| title_full_unstemmed | Intraoperative radiation therapy induces immune response activity after pancreatic surgery |
| title_short | Intraoperative radiation therapy induces immune response activity after pancreatic surgery |
| title_sort | intraoperative radiation therapy induces immune response activity after pancreatic surgery |
| topic | Pancreatic cancer Intraoperative radiation therapy Immune response Cytokine |
| url | https://doi.org/10.1186/s12885-021-08807-3 |
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