Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models
Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen that can cause a variety of infections in humans, such as burn wound infections and infections of the lungs, the bloodstream and surgical site infections. Nosocomial spread is often concurrent with high degrees of antibiotic res...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02603-0 |
| _version_ | 1798038005596815360 |
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| author | Shri Natrajan Arumugam Prasanth Manohar Sunilkumar Sukumaran Sathish Sadagopan Belinda Loh Sebastian Leptihn Ramesh Nachimuthu |
| author_facet | Shri Natrajan Arumugam Prasanth Manohar Sunilkumar Sukumaran Sathish Sadagopan Belinda Loh Sebastian Leptihn Ramesh Nachimuthu |
| author_sort | Shri Natrajan Arumugam |
| collection | DOAJ |
| description | Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen that can cause a variety of infections in humans, such as burn wound infections and infections of the lungs, the bloodstream and surgical site infections. Nosocomial spread is often concurrent with high degrees of antibiotic resistance. Such resistant strains are difficult to treat, and in some cases, even reserved antibiotics are ineffective. A particularly promising therapy to combat infections of resistant bacteria is the deployment of bacteriophages, known as phage therapy. In this work, we evaluated the in vivo efficacy of two Pseudomonas phages in bacteremia mice models. For this study, non-neutropenic mice (BalB/C) were infected with P. aeruginosa AB030 strain and treated using two bacteriophages, AP025 and AP006. Results The results showed that a single dose of phages at higher concentrations, bacteria: phage at 1:10 and 1:100 were effective in eliminating the bloodstream infection and achieving 100% mice survival. Conclusion This study highlights the efficacy of using a single dose of phages to restore mice from bacteremia. |
| first_indexed | 2024-04-11T21:34:19Z |
| format | Article |
| id | doaj.art-caeddcca467740cda437ca92b03f7978 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-11T21:34:19Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-caeddcca467740cda437ca92b03f79782022-12-22T04:01:48ZengBMCBMC Microbiology1471-21802022-08-012211710.1186/s12866-022-02603-0Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice modelsShri Natrajan Arumugam0Prasanth Manohar1Sunilkumar Sukumaran2Sathish Sadagopan3Belinda Loh4Sebastian Leptihn5Ramesh Nachimuthu6Anthem Biosciences Pvt Ltd.Zhejiang University-University of Edinburgh (ZJE) Institute, Zhejiang University, School of MedicineAnthem Biosciences Pvt Ltd.Anthem Biosciences Pvt Ltd.Fraunhofer Institute for Cell Therapy and Immunology (IZI), Department of Antimicrobial BiotechnologyZhejiang University-University of Edinburgh (ZJE) Institute, Zhejiang University, School of MedicineAntibiotic Resistance and Phage Therapy Laboratory, Vellore Institute of Technology, School of Biosciences and TechnologyAbstract Background Pseudomonas aeruginosa is an opportunistic pathogen that can cause a variety of infections in humans, such as burn wound infections and infections of the lungs, the bloodstream and surgical site infections. Nosocomial spread is often concurrent with high degrees of antibiotic resistance. Such resistant strains are difficult to treat, and in some cases, even reserved antibiotics are ineffective. A particularly promising therapy to combat infections of resistant bacteria is the deployment of bacteriophages, known as phage therapy. In this work, we evaluated the in vivo efficacy of two Pseudomonas phages in bacteremia mice models. For this study, non-neutropenic mice (BalB/C) were infected with P. aeruginosa AB030 strain and treated using two bacteriophages, AP025 and AP006. Results The results showed that a single dose of phages at higher concentrations, bacteria: phage at 1:10 and 1:100 were effective in eliminating the bloodstream infection and achieving 100% mice survival. Conclusion This study highlights the efficacy of using a single dose of phages to restore mice from bacteremia.https://doi.org/10.1186/s12866-022-02603-0Pseudomonas aeruginosaAntibiotic resistancePhage therapyBloodstream infectionsIn vivo modelsPhage efficacy |
| spellingShingle | Shri Natrajan Arumugam Prasanth Manohar Sunilkumar Sukumaran Sathish Sadagopan Belinda Loh Sebastian Leptihn Ramesh Nachimuthu Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models BMC Microbiology Pseudomonas aeruginosa Antibiotic resistance Phage therapy Bloodstream infections In vivo models Phage efficacy |
| title | Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models |
| title_full | Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models |
| title_fullStr | Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models |
| title_full_unstemmed | Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models |
| title_short | Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models |
| title_sort | antibacterial efficacy of lytic phages against multidrug resistant pseudomonas aeruginosa infections in bacteraemia mice models |
| topic | Pseudomonas aeruginosa Antibiotic resistance Phage therapy Bloodstream infections In vivo models Phage efficacy |
| url | https://doi.org/10.1186/s12866-022-02603-0 |
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