Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella
Worldwide distributed coccidiosis is caused by infection of both Eimeria species and Cystoisospora in the host intestine and causes huge economic losses to the livestock industry, especially the poultry industry. The control of such diseases relies mainly on chemoprophylaxis with anticoccidials, whi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | International Journal for Parasitology: Drugs and Drug Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211320723000179 |
| _version_ | 1797771590621986816 |
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| author | Hongtao Zhang Lei Zhang Ganglin Ren Hongbin Si Xingju Song Xianyong Liu Xun Suo Dandan Hu |
| author_facet | Hongtao Zhang Lei Zhang Ganglin Ren Hongbin Si Xingju Song Xianyong Liu Xun Suo Dandan Hu |
| author_sort | Hongtao Zhang |
| collection | DOAJ |
| description | Worldwide distributed coccidiosis is caused by infection of both Eimeria species and Cystoisospora in the host intestine and causes huge economic losses to the livestock industry, especially the poultry industry. The control of such diseases relies mainly on chemoprophylaxis with anticoccidials, which has led to a very common drug resistance in this field. However, the genetic mechanisms underlying resistance to many anticoccidial drugs remain unknown. In this study, strains of E. tenella resistant to 250 mg/kg monensin were generated and characterized. Forward genetic approaches based on pooled genome sequencing, including experimental evolution and linkage group selection, were used to locate candidate targets responsible for resistance to monensin and diclazuril in E. tenella. A total of 16 nonsynonymous mutants in protein-coding genes were identified in monensin-resistant strains, and two genomic regions with strong selection signals were also detected in diclazuril-resistant strains. Our study reveals the genetic characterization of the experimental evolution and linkage group selection in Eimeria species, and also provides important information that contributes to the understanding of the molecular mechanism of drug resistance in coccidia. |
| first_indexed | 2024-03-12T21:39:50Z |
| format | Article |
| id | doaj.art-caee3aadc29e44a2a2b4787e1e4100f2 |
| institution | Directory Open Access Journal |
| issn | 2211-3207 |
| language | English |
| last_indexed | 2024-03-12T21:39:50Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Drugs and Drug Resistance |
| spelling | doaj.art-caee3aadc29e44a2a2b4787e1e4100f22023-07-27T05:55:48ZengElsevierInternational Journal for Parasitology: Drugs and Drug Resistance2211-32072023-08-01224451Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenellaHongtao Zhang0Lei Zhang1Ganglin Ren2Hongbin Si3Xingju Song4Xianyong Liu5Xun Suo6Dandan Hu7College of Animal Science and Technology, Guangxi University, ChinaCollege of Animal Science and Technology, Guangxi University, ChinaCollege of Animal Science and Technology, Guangxi University, ChinaCollege of Animal Science and Technology, Guangxi University, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, 530004, ChinaCollege of Animal Science and Technology, Guangxi University, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, 530004, ChinaKey Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaKey Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaCollege of Animal Science and Technology, Guangxi University, China; Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, China; Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Nanning, 530004, China; Corresponding author. College of Animal Science and Technology, Guangxi University, China.Worldwide distributed coccidiosis is caused by infection of both Eimeria species and Cystoisospora in the host intestine and causes huge economic losses to the livestock industry, especially the poultry industry. The control of such diseases relies mainly on chemoprophylaxis with anticoccidials, which has led to a very common drug resistance in this field. However, the genetic mechanisms underlying resistance to many anticoccidial drugs remain unknown. In this study, strains of E. tenella resistant to 250 mg/kg monensin were generated and characterized. Forward genetic approaches based on pooled genome sequencing, including experimental evolution and linkage group selection, were used to locate candidate targets responsible for resistance to monensin and diclazuril in E. tenella. A total of 16 nonsynonymous mutants in protein-coding genes were identified in monensin-resistant strains, and two genomic regions with strong selection signals were also detected in diclazuril-resistant strains. Our study reveals the genetic characterization of the experimental evolution and linkage group selection in Eimeria species, and also provides important information that contributes to the understanding of the molecular mechanism of drug resistance in coccidia.http://www.sciencedirect.com/science/article/pii/S2211320723000179EimeriaWGSMonensinDiclazurilLinkage group selection |
| spellingShingle | Hongtao Zhang Lei Zhang Ganglin Ren Hongbin Si Xingju Song Xianyong Liu Xun Suo Dandan Hu Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella International Journal for Parasitology: Drugs and Drug Resistance Eimeria WGS Monensin Diclazuril Linkage group selection |
| title | Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella |
| title_full | Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella |
| title_fullStr | Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella |
| title_full_unstemmed | Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella |
| title_short | Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella |
| title_sort | forward genetic analysis of monensin and diclazuril resistance in eimeria tenella |
| topic | Eimeria WGS Monensin Diclazuril Linkage group selection |
| url | http://www.sciencedirect.com/science/article/pii/S2211320723000179 |
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