Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth
Precise classification of bacteria facilitates prediction of their ecological niche. The genus <i>Enterobacter</i> includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of <i>Enterobacter</i>...
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/575 |
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| author | Ge Gao Yan Zhang Shaofang Niu Yu Chen Shaojing Wang Nusratgul Anwar Shuai Chen Guoqiang Li Ting Ma |
| author_facet | Ge Gao Yan Zhang Shaofang Niu Yu Chen Shaojing Wang Nusratgul Anwar Shuai Chen Guoqiang Li Ting Ma |
| author_sort | Ge Gao |
| collection | DOAJ |
| description | Precise classification of bacteria facilitates prediction of their ecological niche. The genus <i>Enterobacter</i> includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of <i>Enterobacter</i> FY-07 (FY-07), a strain that has many plant-growth-promoting traits and produces bacterial cellulose (BC), to the <i>Kosakonia</i> genera. To re-examine the taxonomic position of FY-07, a polyphasic approach including 16S rRNA gene sequence analysis, ATP synthase β subunit (<i>atpD</i>) gene sequence analysis, DNA gyrase (<i>gyrB</i>) gene sequence analysis, initiation translation factor 2 (<i>infB</i>) gene sequence analysis, RNA polymerase β subunit (<i>rpoB</i>) gene sequence analysis, determination of DNA G + C content, average nucleotide identity based on BLAST, in silico DNA–DNA hybridization and analysis of phenotypic features was applied. This polyphasic analysis suggested that <i>Enterobacter</i> sp. FY-07 should be reclassified as <i>Kosakonia oryzendophytica</i> FY-07. In addition, the potential of FY-07 to promote plant growth was also investigated by detecting related traits and the colonization of FY-07 in rice roots. |
| first_indexed | 2024-03-09T13:15:52Z |
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| id | doaj.art-caf59b5301594fb9ad09cb843262b5c7 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:15:52Z |
| publishDate | 2022-03-01 |
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| series | Microorganisms |
| spelling | doaj.art-caf59b5301594fb9ad09cb843262b5c72023-11-30T21:36:17ZengMDPI AGMicroorganisms2076-26072022-03-0110357510.3390/microorganisms10030575Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant GrowthGe Gao0Yan Zhang1Shaofang Niu2Yu Chen3Shaojing Wang4Nusratgul Anwar5Shuai Chen6Guoqiang Li7Ting Ma8Key Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaKey Laboratory of Molecular Microbiology and Technology, College of Life Sciences, Nankai University, Ministry of Education, Tianjin 300071, ChinaPrecise classification of bacteria facilitates prediction of their ecological niche. The genus <i>Enterobacter</i> includes pathogens of plants and animals but also beneficial bacteria that may require reclassification. Here, we propose reclassification of <i>Enterobacter</i> FY-07 (FY-07), a strain that has many plant-growth-promoting traits and produces bacterial cellulose (BC), to the <i>Kosakonia</i> genera. To re-examine the taxonomic position of FY-07, a polyphasic approach including 16S rRNA gene sequence analysis, ATP synthase β subunit (<i>atpD</i>) gene sequence analysis, DNA gyrase (<i>gyrB</i>) gene sequence analysis, initiation translation factor 2 (<i>infB</i>) gene sequence analysis, RNA polymerase β subunit (<i>rpoB</i>) gene sequence analysis, determination of DNA G + C content, average nucleotide identity based on BLAST, in silico DNA–DNA hybridization and analysis of phenotypic features was applied. This polyphasic analysis suggested that <i>Enterobacter</i> sp. FY-07 should be reclassified as <i>Kosakonia oryzendophytica</i> FY-07. In addition, the potential of FY-07 to promote plant growth was also investigated by detecting related traits and the colonization of FY-07 in rice roots.https://www.mdpi.com/2076-2607/10/3/575<i>Enterobacter</i><i>Kosakonia</i>reclassificationFY-07promote plant growth |
| spellingShingle | Ge Gao Yan Zhang Shaofang Niu Yu Chen Shaojing Wang Nusratgul Anwar Shuai Chen Guoqiang Li Ting Ma Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth Microorganisms <i>Enterobacter</i> <i>Kosakonia</i> reclassification FY-07 promote plant growth |
| title | Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth |
| title_full | Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth |
| title_fullStr | Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth |
| title_full_unstemmed | Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth |
| title_short | Reclassification of <i>Enterobacter</i> sp. FY-07 as <i>Kosakonia oryzendophytica</i> FY-07 and Its Potential to Promote Plant Growth |
| title_sort | reclassification of i enterobacter i sp fy 07 as i kosakonia oryzendophytica i fy 07 and its potential to promote plant growth |
| topic | <i>Enterobacter</i> <i>Kosakonia</i> reclassification FY-07 promote plant growth |
| url | https://www.mdpi.com/2076-2607/10/3/575 |
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