Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets
Abstract Seaweeds, such as Laminaria digitata, are a sustainable alternative to conventional feedstuffs for weaned piglet diets, improving their health and mitigating environmental impacts. L. digitata has a complex cell wall that can be difficult for monogastrics to digest. However, carbohydrate-ac...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33835-3 |
| _version_ | 1797841104512483328 |
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| author | David M. Ribeiro Rui M. A. Pinto Paula A. Lopes José M. Pestana Cristina M. Alfaia Mónica M. Costa Daniela F. P. Carvalho Miguel P. Mourato André M. de Almeida João P. B. Freire José A. M. Prates |
| author_facet | David M. Ribeiro Rui M. A. Pinto Paula A. Lopes José M. Pestana Cristina M. Alfaia Mónica M. Costa Daniela F. P. Carvalho Miguel P. Mourato André M. de Almeida João P. B. Freire José A. M. Prates |
| author_sort | David M. Ribeiro |
| collection | DOAJ |
| description | Abstract Seaweeds, such as Laminaria digitata, are a sustainable alternative to conventional feedstuffs for weaned piglet diets, improving their health and mitigating environmental impacts. L. digitata has a complex cell wall that can be difficult for monogastrics to digest. However, carbohydrate-active enzymes (CAZymes) such as Rovabio® Excel AP and alginate lyase can help break down these polysaccharides and render intracellular nutrients more accessible. This study aimed to evaluate the impact of 10% L. digitata feed inclusion and CAZyme supplementation on piglet blood cells, serum metabolites, liver lipid and mineral profiles. Forty weaned piglets were randomly assigned to one of four diets (n = 10 each): a control diet, 10% L. digitata (LA), 10% L. digitata + 0.005% Rovabio® Excel AP (LAR), and 10% L. digitata + 0.01% alginate lyase (LAL). After two weeks of trial, animals were slaughtered and liver and blood serum samples taken for analysis. The results showed that the LA and LAL diets increased blood lymphocytes, IgG and IgM, and decreased serum lipids, improving both cellular and humoral immune response and cardiovascular health. Dietary CAZymes reversed the anti-inflammatory and hematopoietic effects. Additionally, cortisol levels were reduced with seaweed inclusion compared to the control diet (P < 0.001). In the liver, total n-3 PUFA and n-6/n-3 ratio were increased and decreased, respectively, due to eicosapentaenoic acid and α-linolenic acid accumulation (P < 0.001). However, total liver mineral content was incorporated to a lesser extent with the combined seaweed and enzyme diets (P < 0.001), potentially indicating a negative effect on mineral bioavailability. Overall, results suggest that a 10% L. digitata inclusion can effectively improve piglet health by reducing stress during weaning, without the need for dietary CAZymes. |
| first_indexed | 2024-04-09T16:25:37Z |
| format | Article |
| id | doaj.art-caf81825c78c48db828bfea1769a4b60 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T16:25:37Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-caf81825c78c48db828bfea1769a4b602023-04-23T11:13:21ZengNature PortfolioScientific Reports2045-23222023-04-0113111110.1038/s41598-023-33835-3Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned pigletsDavid M. Ribeiro0Rui M. A. Pinto1Paula A. Lopes2José M. Pestana3Cristina M. Alfaia4Mónica M. Costa5Daniela F. P. Carvalho6Miguel P. Mourato7André M. de Almeida8João P. B. Freire9José A. M. Prates10LEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de LisboaiMED.UL, Faculdade de Farmácia, Universidade de LisboaCIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de LisboaCIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de LisboaCIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de LisboaCIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de LisboaLEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de LisboaLEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de LisboaLEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de LisboaLEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de LisboaCIISA-Centre for Interdisciplinary Research in Animal Health, Faculdade de Medicina Veterinária, Universidade de LisboaAbstract Seaweeds, such as Laminaria digitata, are a sustainable alternative to conventional feedstuffs for weaned piglet diets, improving their health and mitigating environmental impacts. L. digitata has a complex cell wall that can be difficult for monogastrics to digest. However, carbohydrate-active enzymes (CAZymes) such as Rovabio® Excel AP and alginate lyase can help break down these polysaccharides and render intracellular nutrients more accessible. This study aimed to evaluate the impact of 10% L. digitata feed inclusion and CAZyme supplementation on piglet blood cells, serum metabolites, liver lipid and mineral profiles. Forty weaned piglets were randomly assigned to one of four diets (n = 10 each): a control diet, 10% L. digitata (LA), 10% L. digitata + 0.005% Rovabio® Excel AP (LAR), and 10% L. digitata + 0.01% alginate lyase (LAL). After two weeks of trial, animals were slaughtered and liver and blood serum samples taken for analysis. The results showed that the LA and LAL diets increased blood lymphocytes, IgG and IgM, and decreased serum lipids, improving both cellular and humoral immune response and cardiovascular health. Dietary CAZymes reversed the anti-inflammatory and hematopoietic effects. Additionally, cortisol levels were reduced with seaweed inclusion compared to the control diet (P < 0.001). In the liver, total n-3 PUFA and n-6/n-3 ratio were increased and decreased, respectively, due to eicosapentaenoic acid and α-linolenic acid accumulation (P < 0.001). However, total liver mineral content was incorporated to a lesser extent with the combined seaweed and enzyme diets (P < 0.001), potentially indicating a negative effect on mineral bioavailability. Overall, results suggest that a 10% L. digitata inclusion can effectively improve piglet health by reducing stress during weaning, without the need for dietary CAZymes.https://doi.org/10.1038/s41598-023-33835-3 |
| spellingShingle | David M. Ribeiro Rui M. A. Pinto Paula A. Lopes José M. Pestana Cristina M. Alfaia Mónica M. Costa Daniela F. P. Carvalho Miguel P. Mourato André M. de Almeida João P. B. Freire José A. M. Prates Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets Scientific Reports |
| title | Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets |
| title_full | Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets |
| title_fullStr | Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets |
| title_full_unstemmed | Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets |
| title_short | Effect of Laminaria digitata dietary inclusion and CAZyme supplementation on blood cells, serum metabolites and hepatic lipids and minerals of weaned piglets |
| title_sort | effect of laminaria digitata dietary inclusion and cazyme supplementation on blood cells serum metabolites and hepatic lipids and minerals of weaned piglets |
| url | https://doi.org/10.1038/s41598-023-33835-3 |
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