lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition
Esophageal squamous cell carcinoma (ESCC) is a common cancer occurring in males and females worldwide. Accumulating evidence continues to highlight the crucial roles of long non-coding RNAs (lncRNAs) in the process of tumorigenesis. However, the regulatory mechanism of lncRNAs in ESCC remains unclea...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-06-01
|
| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S216225312030055X |
| _version_ | 1818773209270452224 |
|---|---|
| author | Jun-Qi Liu Ming Deng Nan-Nan Xue Ting-Xuan Li Yue-Xin Guo Liang Gao Di Zhao Rui-Tai Fan |
| author_facet | Jun-Qi Liu Ming Deng Nan-Nan Xue Ting-Xuan Li Yue-Xin Guo Liang Gao Di Zhao Rui-Tai Fan |
| author_sort | Jun-Qi Liu |
| collection | DOAJ |
| description | Esophageal squamous cell carcinoma (ESCC) is a common cancer occurring in males and females worldwide. Accumulating evidence continues to highlight the crucial roles of long non-coding RNAs (lncRNAs) in the process of tumorigenesis. However, the regulatory mechanism of lncRNAs in ESCC remains unclear. The aim of this study is to elucidate the role of lncRNA Krüppel-like factor 3 antisense RNA 1 (KLF3-AS1) in ESCC by regulating miR-185-5p and KLF3. Initially, ESCC cell spheres with stem cell-like properties were prepared by suspension culture, and subsequently characterized by assessing colony formation ability and stem cell markers. LncRNA KLF3-AS1 was found to be poorly expressed in ESCC and could upregulate the expression of KLF3 by binding to miR-185-5p. lncRNA KLF3-AS1 upregulation was observed to inhibit miR-185-5p, thereby contributing to decreased expression of SOX2 and Oct4 (octamer-binding transcription factor 4). Furthermore, enhancement of lncRNA KLF3-AS1 resulted in reduced colony formation ability, cell invasion and migration, and tumor volume in vivo while promoting cell apoptosis in ESCC through downregulation of miR-185-5p. Collectively, this study indicated that lncRNA KLF3-AS1 inhibited ESCC cell invasion and migration by impairing miR-185-5p-mediated inhibition of KLF3, highlighting a promising novel potential target for ESCC treatment. |
| first_indexed | 2024-12-18T10:21:37Z |
| format | Article |
| id | doaj.art-caf87ee725c1454c849c1e82eed4b920 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-18T10:21:37Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-caf87ee725c1454c849c1e82eed4b9202022-12-21T21:11:08ZengElsevierMolecular Therapy: Nucleic Acids2162-25312020-06-0120231241lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 InhibitionJun-Qi Liu0Ming Deng1Nan-Nan Xue2Ting-Xuan Li3Yue-Xin Guo4Liang Gao5Di Zhao6Rui-Tai Fan7Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. ChinaDepartment of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, P.R. ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. ChinaDepartment of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. ChinaCenter of Experimental Orthopaedics, Saarland University, Kirrberger Strasse, Building 37, 66421 Homburg, GermanyDepartment of Endocrinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China; Corresponding author: Di Zhao, Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Erqi District, Zhengzhou 450000, Henan Province, P.R. China.Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China; Corresponding author: Rui-Tai Fan, Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Erqi District, Zhengzhou 450000, Henan Province, P.R. China.Esophageal squamous cell carcinoma (ESCC) is a common cancer occurring in males and females worldwide. Accumulating evidence continues to highlight the crucial roles of long non-coding RNAs (lncRNAs) in the process of tumorigenesis. However, the regulatory mechanism of lncRNAs in ESCC remains unclear. The aim of this study is to elucidate the role of lncRNA Krüppel-like factor 3 antisense RNA 1 (KLF3-AS1) in ESCC by regulating miR-185-5p and KLF3. Initially, ESCC cell spheres with stem cell-like properties were prepared by suspension culture, and subsequently characterized by assessing colony formation ability and stem cell markers. LncRNA KLF3-AS1 was found to be poorly expressed in ESCC and could upregulate the expression of KLF3 by binding to miR-185-5p. lncRNA KLF3-AS1 upregulation was observed to inhibit miR-185-5p, thereby contributing to decreased expression of SOX2 and Oct4 (octamer-binding transcription factor 4). Furthermore, enhancement of lncRNA KLF3-AS1 resulted in reduced colony formation ability, cell invasion and migration, and tumor volume in vivo while promoting cell apoptosis in ESCC through downregulation of miR-185-5p. Collectively, this study indicated that lncRNA KLF3-AS1 inhibited ESCC cell invasion and migration by impairing miR-185-5p-mediated inhibition of KLF3, highlighting a promising novel potential target for ESCC treatment.http://www.sciencedirect.com/science/article/pii/S216225312030055Xlong non-coding RNA Krüppel-like factor 3 antisense RNA 1competing endogenous RNAmiR-185-5pKrüppel-like factor 3esophageal squamous cell carcinoma stem cellsbiological characteristics |
| spellingShingle | Jun-Qi Liu Ming Deng Nan-Nan Xue Ting-Xuan Li Yue-Xin Guo Liang Gao Di Zhao Rui-Tai Fan lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition Molecular Therapy: Nucleic Acids long non-coding RNA Krüppel-like factor 3 antisense RNA 1 competing endogenous RNA miR-185-5p Krüppel-like factor 3 esophageal squamous cell carcinoma stem cells biological characteristics |
| title | lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition |
| title_full | lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition |
| title_fullStr | lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition |
| title_full_unstemmed | lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition |
| title_short | lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition |
| title_sort | lncrna klf3 as1 suppresses cell migration and invasion in escc by impairing mir 185 5p targeted klf3 inhibition |
| topic | long non-coding RNA Krüppel-like factor 3 antisense RNA 1 competing endogenous RNA miR-185-5p Krüppel-like factor 3 esophageal squamous cell carcinoma stem cells biological characteristics |
| url | http://www.sciencedirect.com/science/article/pii/S216225312030055X |
| work_keys_str_mv | AT junqiliu lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT mingdeng lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT nannanxue lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT tingxuanli lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT yuexinguo lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT lianggao lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT dizhao lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition AT ruitaifan lncrnaklf3as1suppressescellmigrationandinvasioninesccbyimpairingmir1855ptargetedklf3inhibition |