Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons
Short-read sequencing methods cannot delineate internal exon composition and alternative splicing events of long and multi-exon circular RNAs (circRNAs). Here the authors provide a global map of full-length circRNAs by long-read sequencing in human and mouse brain samples.
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24975-z |
| _version_ | 1818841223652179968 |
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| author | Karim Rahimi Morten T. Venø Daniel M. Dupont Jørgen Kjems |
| author_facet | Karim Rahimi Morten T. Venø Daniel M. Dupont Jørgen Kjems |
| author_sort | Karim Rahimi |
| collection | DOAJ |
| description | Short-read sequencing methods cannot delineate internal exon composition and alternative splicing events of long and multi-exon circular RNAs (circRNAs). Here the authors provide a global map of full-length circRNAs by long-read sequencing in human and mouse brain samples. |
| first_indexed | 2024-12-19T04:22:40Z |
| format | Article |
| id | doaj.art-caf8add5fb2e4f0f8efcdf021c9ce999 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T04:22:40Z |
| publishDate | 2021-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-caf8add5fb2e4f0f8efcdf021c9ce9992022-12-21T20:36:07ZengNature PortfolioNature Communications2041-17232021-08-0112111510.1038/s41467-021-24975-zNanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexonsKarim Rahimi0Morten T. Venø1Daniel M. Dupont2Jørgen Kjems3Department of Molecular Biology and Genetics (MBG), Aarhus UniversityDepartment of Molecular Biology and Genetics (MBG), Aarhus UniversityInterdisciplinary Nanoscience Center (iNANO), Aarhus UniversityDepartment of Molecular Biology and Genetics (MBG), Aarhus UniversityShort-read sequencing methods cannot delineate internal exon composition and alternative splicing events of long and multi-exon circular RNAs (circRNAs). Here the authors provide a global map of full-length circRNAs by long-read sequencing in human and mouse brain samples.https://doi.org/10.1038/s41467-021-24975-z |
| spellingShingle | Karim Rahimi Morten T. Venø Daniel M. Dupont Jørgen Kjems Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons Nature Communications |
| title | Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons |
| title_full | Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons |
| title_fullStr | Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons |
| title_full_unstemmed | Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons |
| title_short | Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons |
| title_sort | nanopore sequencing of brain derived full length circrnas reveals circrna specific exon usage intron retention and microexons |
| url | https://doi.org/10.1038/s41467-021-24975-z |
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