Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots
<i>Bruguiera gymnorhiza</i> (L.) Lam is a mangrove plant that spread in many parts of the world. Though mangrove plant polyphenols have been reported to exhibit many biological activities, little is known about mangrove plant tannins. To explore the application value of tannins from <...
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MDPI AG
2021-03-01
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| author | Xuelian Liu Ting Chen Qin Wang Jiaai Liu Yuhao Lu Yan Shi |
| author_facet | Xuelian Liu Ting Chen Qin Wang Jiaai Liu Yuhao Lu Yan Shi |
| author_sort | Xuelian Liu |
| collection | DOAJ |
| description | <i>Bruguiera gymnorhiza</i> (L.) Lam is a mangrove plant that spread in many parts of the world. Though mangrove plant polyphenols have been reported to exhibit many biological activities, little is known about mangrove plant tannins. To explore the application value of tannins from <i>B. gymnorhiza</i>, analyses on the structure and biological activity of condensed tannins (CTs) from <i>Bruguiera gymnorhiza</i> (L.) Lam were carried out. The results from <sup>13</sup>C nuclear magnetic resonance (<sup>13</sup>C-NMR) and reversed-phase, high-performance liquid chromatography (RP-HPLC) showed that the CTs were dominated by procyanidins, with a small quantity of prodelphinidins and propelargonidins; and that the monomeric constituents of <i>B. gymnorhiza</i> tannins were catechin/epicatechin, gallocatechin/epigallocatechin and afzelechin/epiafzelechin. The CTs were reversible and mixed competitive inhibitors of tyrosinase and the 50% inhibiting concentration (IC<sub>50</sub>) was estimated to be 123.90 ± 0.140 μg/mL. The antioxidant activities of CTs from <i>B. gymnorhiza</i> leaves were evaluated, the IC<sub>50</sub> for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid diammonium salt) (ABTS) scavenging activities were 88.81 ± 0.135 and 105.03 ± 0.130 μg/mL, respectively, and the ferric ion reducing antioxidant power (FRAP) value was 1052.27 ± 4.17 mgAAE/g. In addition, the results from fresh-keeping assays on fresh-cut lotus root reveal that CTs from <i>B. gymnorhiza</i> had excellent effects on inhibiting the activities of polyphenol oxidase (PPO) and peroxidase (POD), protecting fresh-cut lotus root from the oxidation of total phenolics and malondialdehyde (MDA) content and slowing the increase in total phenol content (TPC) at 4 °C during the whole storage period. Therefore, CTs showed good effects against the browning of fresh-cut lotus root. Together, these results suggested that <i>B. gymnorhiza</i> CTs are promising antibrowning agents for fresh-cut fruits. |
| first_indexed | 2024-03-09T05:37:09Z |
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| spelling | doaj.art-cafbfa2655ce47a38110e7d5de2001c52023-12-03T12:27:53ZengMDPI AGMolecules1420-30492021-03-01265136910.3390/molecules26051369Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus RootsXuelian Liu0Ting Chen1Qin Wang2Jiaai Liu3Yuhao Lu4Yan Shi5School of Life Sciences, Xiamen University, Xiamen 361102, ChinaSchool of Life Sciences, Xiamen University, Xiamen 361102, ChinaSchool of Life Sciences, Xiamen University, Xiamen 361102, ChinaSchool of Life Sciences, Xiamen University, Xiamen 361102, ChinaSchool of Life Sciences, Xiamen University, Xiamen 361102, ChinaSchool of Life Sciences, Xiamen University, Xiamen 361102, China<i>Bruguiera gymnorhiza</i> (L.) Lam is a mangrove plant that spread in many parts of the world. Though mangrove plant polyphenols have been reported to exhibit many biological activities, little is known about mangrove plant tannins. To explore the application value of tannins from <i>B. gymnorhiza</i>, analyses on the structure and biological activity of condensed tannins (CTs) from <i>Bruguiera gymnorhiza</i> (L.) Lam were carried out. The results from <sup>13</sup>C nuclear magnetic resonance (<sup>13</sup>C-NMR) and reversed-phase, high-performance liquid chromatography (RP-HPLC) showed that the CTs were dominated by procyanidins, with a small quantity of prodelphinidins and propelargonidins; and that the monomeric constituents of <i>B. gymnorhiza</i> tannins were catechin/epicatechin, gallocatechin/epigallocatechin and afzelechin/epiafzelechin. The CTs were reversible and mixed competitive inhibitors of tyrosinase and the 50% inhibiting concentration (IC<sub>50</sub>) was estimated to be 123.90 ± 0.140 μg/mL. The antioxidant activities of CTs from <i>B. gymnorhiza</i> leaves were evaluated, the IC<sub>50</sub> for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid diammonium salt) (ABTS) scavenging activities were 88.81 ± 0.135 and 105.03 ± 0.130 μg/mL, respectively, and the ferric ion reducing antioxidant power (FRAP) value was 1052.27 ± 4.17 mgAAE/g. In addition, the results from fresh-keeping assays on fresh-cut lotus root reveal that CTs from <i>B. gymnorhiza</i> had excellent effects on inhibiting the activities of polyphenol oxidase (PPO) and peroxidase (POD), protecting fresh-cut lotus root from the oxidation of total phenolics and malondialdehyde (MDA) content and slowing the increase in total phenol content (TPC) at 4 °C during the whole storage period. Therefore, CTs showed good effects against the browning of fresh-cut lotus root. Together, these results suggested that <i>B. gymnorhiza</i> CTs are promising antibrowning agents for fresh-cut fruits.https://www.mdpi.com/1420-3049/26/5/1369condensed tanninsstructure analysisbiological activitiesfresh-cut lotus rootfresh-keeping |
| spellingShingle | Xuelian Liu Ting Chen Qin Wang Jiaai Liu Yuhao Lu Yan Shi Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots Molecules condensed tannins structure analysis biological activities fresh-cut lotus root fresh-keeping |
| title | Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots |
| title_full | Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots |
| title_fullStr | Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots |
| title_full_unstemmed | Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots |
| title_short | Structure Analysis and Study of Biological Activities of Condensed Tannins from <i>Bruguiera gymnorhiza</i> (L.) Lam and Their Effect on Fresh-Cut Lotus Roots |
| title_sort | structure analysis and study of biological activities of condensed tannins from i bruguiera gymnorhiza i l lam and their effect on fresh cut lotus roots |
| topic | condensed tannins structure analysis biological activities fresh-cut lotus root fresh-keeping |
| url | https://www.mdpi.com/1420-3049/26/5/1369 |
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