BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES
Optimization of the process of biotransformation of hydroquinone into its β-D-glucoside – arbutin, was performed in agitated shoot cultures of Schisandra chinensis. The optimisation involved testing various concentrations of the precursor and different ways of administering it. Arbutin was accumula...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2017-12-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2389 |
| _version_ | 1797394169699762176 |
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| author | Agnieszka Szopa Inga Kwiecień Halina Ekiert |
| author_facet | Agnieszka Szopa Inga Kwiecień Halina Ekiert |
| author_sort | Agnieszka Szopa |
| collection | DOAJ |
| description |
Optimization of the process of biotransformation of hydroquinone into its β-D-glucoside – arbutin, was performed in agitated shoot cultures of Schisandra chinensis. The optimisation involved testing various concentrations of the precursor and different ways of administering it. Arbutin was accumulated mainly in the in vitro cultured biomass (85.2–98.6%). By optimizing the process, a 2.26-fold increase in the overall product content was obtained. The highest amount (17.8 mg·g–1 DW) was found after administering 384 mg·l–1 hydroquinone in a dose divided into two portions. An experiment with the biotransformation of 4-hydroxybenzoic
acid did not produce arbutin but a mixture of two products of glucosylation of the precursor – hydroxybenzoic acid 4-O-β-glucopyranoside and 4-hydroxybenzoic acid β-glucopyranosyl ester. The identity of all biotransformation products was confirmed by 1H-NMR analysis. The results for the production of arbutin by the biotransformation of hydroquinone are of potential practical importance. On the other hand, the fact of confirming the presence of two glucosylation products has a great cognitive value.
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| first_indexed | 2024-03-09T00:15:36Z |
| format | Article |
| id | doaj.art-cb0124bb456744baaeca234a0fc2f9cb |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:15:36Z |
| publishDate | 2017-12-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-cb0124bb456744baaeca234a0fc2f9cb2023-12-12T08:01:15ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052017-12-01166BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURESAgnieszka SzopaInga KwiecieńHalina Ekiert Optimization of the process of biotransformation of hydroquinone into its β-D-glucoside – arbutin, was performed in agitated shoot cultures of Schisandra chinensis. The optimisation involved testing various concentrations of the precursor and different ways of administering it. Arbutin was accumulated mainly in the in vitro cultured biomass (85.2–98.6%). By optimizing the process, a 2.26-fold increase in the overall product content was obtained. The highest amount (17.8 mg·g–1 DW) was found after administering 384 mg·l–1 hydroquinone in a dose divided into two portions. An experiment with the biotransformation of 4-hydroxybenzoic acid did not produce arbutin but a mixture of two products of glucosylation of the precursor – hydroxybenzoic acid 4-O-β-glucopyranoside and 4-hydroxybenzoic acid β-glucopyranosyl ester. The identity of all biotransformation products was confirmed by 1H-NMR analysis. The results for the production of arbutin by the biotransformation of hydroquinone are of potential practical importance. On the other hand, the fact of confirming the presence of two glucosylation products has a great cognitive value. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2389schizandraβ-glucosylationarbutin productionagitated shoot culturesHPLC analysis |
| spellingShingle | Agnieszka Szopa Inga Kwiecień Halina Ekiert BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES Acta Scientiarum Polonorum: Hortorum Cultus schizandra β-glucosylation arbutin production agitated shoot cultures HPLC analysis |
| title | BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES |
| title_full | BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES |
| title_fullStr | BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES |
| title_full_unstemmed | BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES |
| title_short | BIOTRANSFORMATION OF HYDROQUINONE AND 4-HYDROXYBENZOIC ACID IN Schisandra chinensis (CHINESE MAGNOLIA VINE) in vitro CULTURES |
| title_sort | biotransformation of hydroquinone and 4 hydroxybenzoic acid in schisandra chinensis chinese magnolia vine in vitro cultures |
| topic | schizandra β-glucosylation arbutin production agitated shoot cultures HPLC analysis |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2389 |
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