Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis
As the physical properties and functionality of dipeptides differ from those of amino acids, they have attracted attention in metabolomics; however, their functions in vivo have not been clarified in detail. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, and its majo...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/10/11/442 |
| _version_ | 1797549130937008128 |
|---|---|
| author | Hitoshi Ozawa Akiyoshi Hirayama Futaba Shoji Midori Maruyama Kumi Suzuki Hisami Yamanaka-Okumura Hiroshi Tatano Yuji Morine Tomoyoshi Soga Mitsuo Shimada Masaru Tomita |
| author_facet | Hitoshi Ozawa Akiyoshi Hirayama Futaba Shoji Midori Maruyama Kumi Suzuki Hisami Yamanaka-Okumura Hiroshi Tatano Yuji Morine Tomoyoshi Soga Mitsuo Shimada Masaru Tomita |
| author_sort | Hitoshi Ozawa |
| collection | DOAJ |
| description | As the physical properties and functionality of dipeptides differ from those of amino acids, they have attracted attention in metabolomics; however, their functions in vivo have not been clarified in detail. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, and its major cause is chronic hepatitis. This study was conducted to explore tumor-specific dipeptide characteristics by performing comprehensive dipeptide analysis in the tumor and surrounding nontumor tissue of patients with HCC. Dipeptides were analyzed by liquid chromatography tandem mass spectrometry and capillary electrophoresis tandem mass spectrometry. Principal component analysis using 236 detected dipeptides showed differences in the dipeptide profiles between nontumor and tumor tissues; however, no clear difference was observed in etiological comparison. In addition, the N- and C-terminal amino acid compositions of the detected dipeptides significantly differed, suggesting the substrate specificity of enzyme proteins, such as peptidase. Furthermore, hepatitis-derived HCC may show a characteristic dipeptide profile even before tumor formation. These results provide insight into HCC pathogenesis and may help identify novel biomarkers for diagnosis. |
| first_indexed | 2024-03-10T15:10:02Z |
| format | Article |
| id | doaj.art-cb01d272d53742a9a7159b7d51837d9a |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T15:10:02Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-cb01d272d53742a9a7159b7d51837d9a2023-11-20T19:26:06ZengMDPI AGMetabolites2218-19892020-11-01101144210.3390/metabo10110442Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and HepatitisHitoshi Ozawa0Akiyoshi Hirayama1Futaba Shoji2Midori Maruyama3Kumi Suzuki4Hisami Yamanaka-Okumura5Hiroshi Tatano6Yuji Morine7Tomoyoshi Soga8Mitsuo Shimada9Masaru Tomita10Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanDepartment of Clinical Nutrition and Food Management, Graduate School of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8503, JapanDepartment of Clinical Nutrition and Food Management, Graduate School of Biomedical Sciences, Tokushima University Graduate School, Tokushima 770-8503, JapanDepartment of Digestive and Pediatric Surgery, Graduate School of Medical Sciences, Tokushima University, Tokushima 770-8503, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanDepartment of Digestive and Pediatric Surgery, Graduate School of Medical Sciences, Tokushima University, Tokushima 770-8503, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, JapanAs the physical properties and functionality of dipeptides differ from those of amino acids, they have attracted attention in metabolomics; however, their functions in vivo have not been clarified in detail. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, and its major cause is chronic hepatitis. This study was conducted to explore tumor-specific dipeptide characteristics by performing comprehensive dipeptide analysis in the tumor and surrounding nontumor tissue of patients with HCC. Dipeptides were analyzed by liquid chromatography tandem mass spectrometry and capillary electrophoresis tandem mass spectrometry. Principal component analysis using 236 detected dipeptides showed differences in the dipeptide profiles between nontumor and tumor tissues; however, no clear difference was observed in etiological comparison. In addition, the N- and C-terminal amino acid compositions of the detected dipeptides significantly differed, suggesting the substrate specificity of enzyme proteins, such as peptidase. Furthermore, hepatitis-derived HCC may show a characteristic dipeptide profile even before tumor formation. These results provide insight into HCC pathogenesis and may help identify novel biomarkers for diagnosis.https://www.mdpi.com/2218-1989/10/11/442dipeptidehepatocellular carcinomahepatitis B virushepatitis C virusliquid chromatography tandem mass spectrometrycapillary electrophoresis tandem mass spectrometry |
| spellingShingle | Hitoshi Ozawa Akiyoshi Hirayama Futaba Shoji Midori Maruyama Kumi Suzuki Hisami Yamanaka-Okumura Hiroshi Tatano Yuji Morine Tomoyoshi Soga Mitsuo Shimada Masaru Tomita Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis Metabolites dipeptide hepatocellular carcinoma hepatitis B virus hepatitis C virus liquid chromatography tandem mass spectrometry capillary electrophoresis tandem mass spectrometry |
| title | Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis |
| title_full | Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis |
| title_fullStr | Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis |
| title_full_unstemmed | Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis |
| title_short | Comprehensive Dipeptide Analysis Revealed Cancer-Specific Profile in the Liver of Patients with Hepatocellular Carcinoma and Hepatitis |
| title_sort | comprehensive dipeptide analysis revealed cancer specific profile in the liver of patients with hepatocellular carcinoma and hepatitis |
| topic | dipeptide hepatocellular carcinoma hepatitis B virus hepatitis C virus liquid chromatography tandem mass spectrometry capillary electrophoresis tandem mass spectrometry |
| url | https://www.mdpi.com/2218-1989/10/11/442 |
| work_keys_str_mv | AT hitoshiozawa comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT akiyoshihirayama comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT futabashoji comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT midorimaruyama comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT kumisuzuki comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT hisamiyamanakaokumura comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT hiroshitatano comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT yujimorine comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT tomoyoshisoga comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT mitsuoshimada comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis AT masarutomita comprehensivedipeptideanalysisrevealedcancerspecificprofileintheliverofpatientswithhepatocellularcarcinomaandhepatitis |