Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer
Xanthohumol (XN), isoxanthohumol (IX) and 8-prenylnaringenin (8-PN) are important prenylflavonoids present in hops with potential beneficial properties. In this study, we examined differences in the content of XN, IX and 8-PN in hops and beer produced under organic and conventional production regime...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/8/1839 |
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| author | Jelena B. Golubović Ester Heath Iztok Jože Košir Nives Ogrinc Doris Potočnik Lidija Strojnik David Heath |
| author_facet | Jelena B. Golubović Ester Heath Iztok Jože Košir Nives Ogrinc Doris Potočnik Lidija Strojnik David Heath |
| author_sort | Jelena B. Golubović |
| collection | DOAJ |
| description | Xanthohumol (XN), isoxanthohumol (IX) and 8-prenylnaringenin (8-PN) are important prenylflavonoids present in hops with potential beneficial properties. In this study, we examined differences in the content of XN, IX and 8-PN in hops and beer produced under organic and conventional production regimes. A An ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method for analysing XN, IX and 8-PN in hops and beer was developed and validated, with LOQ ranging from 0.5 to 10 ng/mL. Finally, we examined <sup>15</sup>N/<sup>14</sup>N and <sup>12</sup>C/<sup>13</sup>C isotope ratios in the hops and beer using isotope ratio mass spectrometry (IRMS). The results show no statistically significant difference in the content of the selected prenylflavonoids between organic and conventionally produced hops and beer—in the whole sample group, as well as between the matched pairs. Stable isotope analysis indicated that only <i>δ</i><sup>15</sup>N values are statistically higher in organically produced hops and beer. However, the differentiation according to the type of production could not be made solely based on the <i>δ</i><sup>15</sup>N signature, but it could be used to provide supporting evidence. |
| first_indexed | 2024-03-10T08:49:07Z |
| format | Article |
| id | doaj.art-cb0207436ee3445b84d405407a8fd7e4 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T08:49:07Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-cb0207436ee3445b84d405407a8fd7e42023-11-22T07:39:33ZengMDPI AGFoods2304-81582021-08-01108183910.3390/foods10081839Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and BeerJelena B. Golubović0Ester Heath1Iztok Jože Košir2Nives Ogrinc3Doris Potočnik4Lidija Strojnik5David Heath6Jožef Stefan Institute, 1000 Ljubljana, SloveniaJožef Stefan Institute, 1000 Ljubljana, SloveniaInstitute for Hop Research and Brewing, 3310 Žalec, SloveniaJožef Stefan Institute, 1000 Ljubljana, SloveniaJožef Stefan Institute, 1000 Ljubljana, SloveniaJožef Stefan Institute, 1000 Ljubljana, SloveniaJožef Stefan Institute, 1000 Ljubljana, SloveniaXanthohumol (XN), isoxanthohumol (IX) and 8-prenylnaringenin (8-PN) are important prenylflavonoids present in hops with potential beneficial properties. In this study, we examined differences in the content of XN, IX and 8-PN in hops and beer produced under organic and conventional production regimes. A An ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method for analysing XN, IX and 8-PN in hops and beer was developed and validated, with LOQ ranging from 0.5 to 10 ng/mL. Finally, we examined <sup>15</sup>N/<sup>14</sup>N and <sup>12</sup>C/<sup>13</sup>C isotope ratios in the hops and beer using isotope ratio mass spectrometry (IRMS). The results show no statistically significant difference in the content of the selected prenylflavonoids between organic and conventionally produced hops and beer—in the whole sample group, as well as between the matched pairs. Stable isotope analysis indicated that only <i>δ</i><sup>15</sup>N values are statistically higher in organically produced hops and beer. However, the differentiation according to the type of production could not be made solely based on the <i>δ</i><sup>15</sup>N signature, but it could be used to provide supporting evidence.https://www.mdpi.com/2304-8158/10/8/1839beerhopsorganicliquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)phytoestrogenstable isotopes |
| spellingShingle | Jelena B. Golubović Ester Heath Iztok Jože Košir Nives Ogrinc Doris Potočnik Lidija Strojnik David Heath Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer Foods beer hops organic liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) phytoestrogen stable isotopes |
| title | Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer |
| title_full | Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer |
| title_fullStr | Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer |
| title_full_unstemmed | Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer |
| title_short | Differences in the Levels of the Selected Phytoestrogens and Stable Isotopes in Organic vs. Conventional Hops and Beer |
| title_sort | differences in the levels of the selected phytoestrogens and stable isotopes in organic vs conventional hops and beer |
| topic | beer hops organic liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) phytoestrogen stable isotopes |
| url | https://www.mdpi.com/2304-8158/10/8/1839 |
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