Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells
The (-)-<i>Epigallocatechin</i>-<i>gallate</i> (EGCG) metabolite is a natural polyphenol derived from green tea and is associated with antioxidant, biocompatible, and anti-inflammatory effects. Objective: To evaluate the effects of EGCG to promote the odontoblast-like cells d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomimetics |
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| Online Access: | https://www.mdpi.com/2313-7673/8/1/75 |
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| author | Rene Garcia-Contreras Patricia Alejandra Chavez-Granados Carlos Alberto Jurado Benjamin Aranda-Herrera Kelvin I. Afrashtehfar Hamid Nurrohman |
| author_facet | Rene Garcia-Contreras Patricia Alejandra Chavez-Granados Carlos Alberto Jurado Benjamin Aranda-Herrera Kelvin I. Afrashtehfar Hamid Nurrohman |
| author_sort | Rene Garcia-Contreras |
| collection | DOAJ |
| description | The (-)-<i>Epigallocatechin</i>-<i>gallate</i> (EGCG) metabolite is a natural polyphenol derived from green tea and is associated with antioxidant, biocompatible, and anti-inflammatory effects. Objective: To evaluate the effects of EGCG to promote the odontoblast-like cells differentiated from human dental pulp stem cells (hDPSCs); the antimicrobial effects on <i>Escherichia coli</i>, <i>Streptococcus mutans</i>, and <i>Staphylococcus aureus</i>; and improve the adhesion on enamel and dentin by shear bond strength (SBS) and the adhesive remnant index (ARI). Material and methods: hDSPCs were isolated from pulp tissue and immunologically characterized. EEGC dose-response viability was calculated by MTT assay. Odontoblast-like cells were differentiated from hDPSCs and tested for mineral deposition activity by alizarin red, Von Kossa, and collagen/vimentin staining. Antimicrobial assays were performed in the microdilution test. Demineralization of enamel and dentin in teeth was performed, and the adhesion was conducted by incorporating EGCG in an adhesive system and testing with SBS-ARI. The data were analyzed with normalized Shapiro–Wilks test and ANOVA post hoc Tukey test. Results: The hDPSCs were positive to CD105, CD90, and vimentin and negative to CD34. EGCG (3.12 µg/mL) accelerated the differentiation of odontoblast-like cells. <i>Streptococcus mutans</i> exhibited the highest susceptibility < <i>Staphylococcus aureus</i> < <i>Escherichia coli</i>. EGCG increased (<i>p</i> < 0.05) the dentin adhesion, and cohesive failure was the most frequent. Conclusion: (-)-<i>Epigallocatechin</i>-<i>gallate</i> is nontoxic, promotes differentiation into odontoblast-like cells, possesses an antibacterial effect, and increases dentin adhesion. |
| first_indexed | 2024-03-11T06:52:56Z |
| format | Article |
| id | doaj.art-cb021ec7f80e4391bb33081ed1bf086e |
| institution | Directory Open Access Journal |
| issn | 2313-7673 |
| language | English |
| last_indexed | 2024-03-11T06:52:56Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
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| series | Biomimetics |
| spelling | doaj.art-cb021ec7f80e4391bb33081ed1bf086e2023-11-17T09:49:55ZengMDPI AGBiomimetics2313-76732023-02-01817510.3390/biomimetics8010075Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like CellsRene Garcia-Contreras0Patricia Alejandra Chavez-Granados1Carlos Alberto Jurado2Benjamin Aranda-Herrera3Kelvin I. Afrashtehfar4Hamid Nurrohman5Interdisciplinary Research Laboratory, Nanostructures, and Biomaterials Area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico (UNAM), Leon 37684, Guanajuato, MexicoInterdisciplinary Research Laboratory, Nanostructures, and Biomaterials Area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico (UNAM), Leon 37684, Guanajuato, MexicoDepartment of Prosthodontics, The University of Iowa College of Dentistry and Dental Clinics, Iowa City, IA 52242, USAInterdisciplinary Research Laboratory, Nanostructures, and Biomaterials Area, National School of Higher Studies (ENES) Leon, National Autonomous University of Mexico (UNAM), Leon 37684, Guanajuato, MexicoClinical Sciences Department, College of Dentistry, Ajman University, Ajman City P.O. Box 346, United Arab EmiratesMissouri School of Dentistry & Oral Health, A. T. Still University, Kirksville, MO 63501, USAThe (-)-<i>Epigallocatechin</i>-<i>gallate</i> (EGCG) metabolite is a natural polyphenol derived from green tea and is associated with antioxidant, biocompatible, and anti-inflammatory effects. Objective: To evaluate the effects of EGCG to promote the odontoblast-like cells differentiated from human dental pulp stem cells (hDPSCs); the antimicrobial effects on <i>Escherichia coli</i>, <i>Streptococcus mutans</i>, and <i>Staphylococcus aureus</i>; and improve the adhesion on enamel and dentin by shear bond strength (SBS) and the adhesive remnant index (ARI). Material and methods: hDSPCs were isolated from pulp tissue and immunologically characterized. EEGC dose-response viability was calculated by MTT assay. Odontoblast-like cells were differentiated from hDPSCs and tested for mineral deposition activity by alizarin red, Von Kossa, and collagen/vimentin staining. Antimicrobial assays were performed in the microdilution test. Demineralization of enamel and dentin in teeth was performed, and the adhesion was conducted by incorporating EGCG in an adhesive system and testing with SBS-ARI. The data were analyzed with normalized Shapiro–Wilks test and ANOVA post hoc Tukey test. Results: The hDPSCs were positive to CD105, CD90, and vimentin and negative to CD34. EGCG (3.12 µg/mL) accelerated the differentiation of odontoblast-like cells. <i>Streptococcus mutans</i> exhibited the highest susceptibility < <i>Staphylococcus aureus</i> < <i>Escherichia coli</i>. EGCG increased (<i>p</i> < 0.05) the dentin adhesion, and cohesive failure was the most frequent. Conclusion: (-)-<i>Epigallocatechin</i>-<i>gallate</i> is nontoxic, promotes differentiation into odontoblast-like cells, possesses an antibacterial effect, and increases dentin adhesion.https://www.mdpi.com/2313-7673/8/1/75polyphenolsbiomodificationdentin repairresin–dentin bond |
| spellingShingle | Rene Garcia-Contreras Patricia Alejandra Chavez-Granados Carlos Alberto Jurado Benjamin Aranda-Herrera Kelvin I. Afrashtehfar Hamid Nurrohman Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells Biomimetics polyphenols biomodification dentin repair resin–dentin bond |
| title | Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells |
| title_full | Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells |
| title_fullStr | Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells |
| title_full_unstemmed | Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells |
| title_short | Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells |
| title_sort | natural bioactive epigallocatechin gallate promote bond strength and differentiation of odontoblast like cells |
| topic | polyphenols biomodification dentin repair resin–dentin bond |
| url | https://www.mdpi.com/2313-7673/8/1/75 |
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