Approaches for the isolation and long-term expansion of pericytes from human and animal tissues
Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controllin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.1095141/full |
| _version_ | 1828067628059459584 |
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| author | Valeria Vincenza Alvino Khaled Abdelsattar Kassem Mohammed Khaled Abdelsattar Kassem Mohammed Yue Gu Paolo Madeddu |
| author_facet | Valeria Vincenza Alvino Khaled Abdelsattar Kassem Mohammed Khaled Abdelsattar Kassem Mohammed Yue Gu Paolo Madeddu |
| author_sort | Valeria Vincenza Alvino |
| collection | DOAJ |
| description | Pericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair. |
| first_indexed | 2024-04-10T23:49:34Z |
| format | Article |
| id | doaj.art-cb038d33178a4eab922542bb659d2680 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-10T23:49:34Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-cb038d33178a4eab922542bb659d26802023-01-10T21:46:44ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-01-01910.3389/fcvm.2022.10951411095141Approaches for the isolation and long-term expansion of pericytes from human and animal tissuesValeria Vincenza Alvino0Khaled Abdelsattar Kassem Mohammed1Khaled Abdelsattar Kassem Mohammed2Yue Gu3Paolo Madeddu4Bristol Heart Institute, University of Bristol, Bristol, United KingdomBristol Heart Institute, University of Bristol, Bristol, United KingdomDepartment of Cardiothoracic Surgery, Faculty of Medicine, Assiut University, Asyut, EgyptBristol Heart Institute, University of Bristol, Bristol, United KingdomBristol Heart Institute, University of Bristol, Bristol, United KingdomPericytes surround capillaries in every organ of the human body. They are also present around the vasa vasorum, the small blood vessels that supply the walls of larger arteries and veins. The clinical interest in pericytes is rapidly growing, with the recognition of their crucial roles in controlling vascular function and possible therapeutic applications in regenerative medicine. Nonetheless, discrepancies in methods used to define, isolate, and expand pericytes are common and may affect reproducibility. Separating pure pericyte preparations from the continuum of perivascular mesenchymal cells is challenging. Moreover, variations in functional behavior and antigenic phenotype in response to environmental stimuli make it difficult to formulate an unequivocal definition of bona fide pericytes. Very few attempts were made to develop pericytes as a clinical-grade product. Therefore, this review is devoted to appraising current methodologies’ pros and cons and proposing standardization and harmonization improvements. We highlight the importance of developing upgraded protocols to create therapeutic pericyte products according to the regulatory guidelines for clinical manufacturing. Finally, we describe how integrating RNA-seq techniques with single-cell spatial analysis, and functional assays may help realize the full potential of pericytes in health, disease, and tissue repair.https://www.frontiersin.org/articles/10.3389/fcvm.2022.1095141/fullpericytesregenerative medicinemethodsisolation and expansioncardiovascular |
| spellingShingle | Valeria Vincenza Alvino Khaled Abdelsattar Kassem Mohammed Khaled Abdelsattar Kassem Mohammed Yue Gu Paolo Madeddu Approaches for the isolation and long-term expansion of pericytes from human and animal tissues Frontiers in Cardiovascular Medicine pericytes regenerative medicine methods isolation and expansion cardiovascular |
| title | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
| title_full | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
| title_fullStr | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
| title_full_unstemmed | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
| title_short | Approaches for the isolation and long-term expansion of pericytes from human and animal tissues |
| title_sort | approaches for the isolation and long term expansion of pericytes from human and animal tissues |
| topic | pericytes regenerative medicine methods isolation and expansion cardiovascular |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.1095141/full |
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