Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS)
Fluorescence is not frequently used as a tool for investigating the photocycles of rhodopsins, largely because of the low quantum yield of the retinal chromophore. However, a new class of genetically encoded voltage sensors is based upon rhodopsins and their fluorescence. The first such sensor repor...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-09-01
|
| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnins.2015.00315/full |
| _version_ | 1818453300140310528 |
|---|---|
| author | Jay eNadeau |
| author_facet | Jay eNadeau |
| author_sort | Jay eNadeau |
| collection | DOAJ |
| description | Fluorescence is not frequently used as a tool for investigating the photocycles of rhodopsins, largely because of the low quantum yield of the retinal chromophore. However, a new class of genetically encoded voltage sensors is based upon rhodopsins and their fluorescence. The first such sensor reported in the literature was the proteorhodopsin optical proton sensor (PROPS), which is capable of indicating membrane voltage changes in bacteria by means of changes in fluorescence. However, the properties of this fluorescence, such as its lifetime decay components and its origin in the protein photocycle, remain unknown. This paper reports steady-state and nanoscale time-resolved emission of this protein expressed in two strains of Escherichia coli, before and after membrane depolarization. The voltage-dependence of a particularly long lifetime component is established. Additional work to improve quantum yields and improve the general utility of PROPS is suggested. |
| first_indexed | 2024-12-14T21:36:47Z |
| format | Article |
| id | doaj.art-cb0504534827409a9bb5395ab738009b |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-14T21:36:47Z |
| publishDate | 2015-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-cb0504534827409a9bb5395ab738009b2022-12-21T22:46:34ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2015-09-01910.3389/fnins.2015.00315158355Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS)Jay eNadeau0California Institute of TechnologyFluorescence is not frequently used as a tool for investigating the photocycles of rhodopsins, largely because of the low quantum yield of the retinal chromophore. However, a new class of genetically encoded voltage sensors is based upon rhodopsins and their fluorescence. The first such sensor reported in the literature was the proteorhodopsin optical proton sensor (PROPS), which is capable of indicating membrane voltage changes in bacteria by means of changes in fluorescence. However, the properties of this fluorescence, such as its lifetime decay components and its origin in the protein photocycle, remain unknown. This paper reports steady-state and nanoscale time-resolved emission of this protein expressed in two strains of Escherichia coli, before and after membrane depolarization. The voltage-dependence of a particularly long lifetime component is established. Additional work to improve quantum yields and improve the general utility of PROPS is suggested.http://journal.frontiersin.org/Journal/10.3389/fnins.2015.00315/fullElectrophysiologyoptical recordingGenetically-encoded voltage sensorstime-resolved fluorescenceproteorhodopin |
| spellingShingle | Jay eNadeau Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS) Frontiers in Neuroscience Electrophysiology optical recording Genetically-encoded voltage sensors time-resolved fluorescence proteorhodopin |
| title | Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS) |
| title_full | Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS) |
| title_fullStr | Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS) |
| title_full_unstemmed | Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS) |
| title_short | Initial Photophysical Characterization of the Proteorhodopsin Optical Proton Sensor (PROPS) |
| title_sort | initial photophysical characterization of the proteorhodopsin optical proton sensor props |
| topic | Electrophysiology optical recording Genetically-encoded voltage sensors time-resolved fluorescence proteorhodopin |
| url | http://journal.frontiersin.org/Journal/10.3389/fnins.2015.00315/full |
| work_keys_str_mv | AT jayenadeau initialphotophysicalcharacterizationoftheproteorhodopsinopticalprotonsensorprops |