Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report
Abstract Background We report a rare case of Mammalian orthoreovirus (MRV) infection in a child with a primary immunodeficiency (PID). Infections with Mammalian orthoreovirus are very rare and probably of zoonotic origin. Only a few cases have been described so far, including one with similar pathog...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-018-2946-7 |
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| author | Dagmara W. Lewandowska Riccarda Capaul Seraina Prader Osvaldo Zagordi Fabienne-Desirée Geissberger Martin Kügler Marcus Knorr Christoph Berger Tayfun Güngör Janine Reichenbach Cyril Shah Jürg Böni Andrea Zbinden Alexandra Trkola Jana Pachlopnik Schmid Michael Huber |
| author_facet | Dagmara W. Lewandowska Riccarda Capaul Seraina Prader Osvaldo Zagordi Fabienne-Desirée Geissberger Martin Kügler Marcus Knorr Christoph Berger Tayfun Güngör Janine Reichenbach Cyril Shah Jürg Böni Andrea Zbinden Alexandra Trkola Jana Pachlopnik Schmid Michael Huber |
| author_sort | Dagmara W. Lewandowska |
| collection | DOAJ |
| description | Abstract Background We report a rare case of Mammalian orthoreovirus (MRV) infection in a child with a primary immunodeficiency (PID). Infections with Mammalian orthoreovirus are very rare and probably of zoonotic origin. Only a few cases have been described so far, including one with similar pathogenesis as in our case. Case presentation The patient, age 11, presented with flu-like symptoms and persistent severe diarrhea. Enterovirus has been detected over several months, however, exact typing of a positive cell culture remained inconclusive. Unbiased metagenomic sequencing then detected MRV in stool samples from several time points. The sequencing approach further revealed co-infection with a recombinant Coxsackievirus and Adenovirus. MRV-specific antibodies detected by immunofluorescence proved that the patient seroconverted. Conclusion This case highlights the potential of unbiased metagenomic sequencing in supplementing routine diagnostic methods, especially in situations of chronic infection with multiple viruses as seen here in an immunocompromised host. The origin, transmission routes and implications of MRV infection in humans merit further investigation. |
| first_indexed | 2024-12-23T04:40:40Z |
| format | Article |
| id | doaj.art-cb0761699b334f39883b93fa8c7e3b4c |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-23T04:40:40Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-cb0761699b334f39883b93fa8c7e3b4c2022-12-21T17:59:46ZengBMCBMC Infectious Diseases1471-23342018-01-011811510.1186/s12879-018-2946-7Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case reportDagmara W. Lewandowska0Riccarda Capaul1Seraina Prader2Osvaldo Zagordi3Fabienne-Desirée Geissberger4Martin Kügler5Marcus Knorr6Christoph Berger7Tayfun Güngör8Janine Reichenbach9Cyril Shah10Jürg Böni11Andrea Zbinden12Alexandra Trkola13Jana Pachlopnik Schmid14Michael Huber15Institute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichDivision of Immunology, University Children’s Hospital ZurichInstitute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichDivision of Infectious Diseases and Hospital Epidemiology, University Children’s Hospital ZurichDivision of Stem Cell Transplantation, University Children’s Hospital ZurichDivision of Immunology, University Children’s Hospital ZurichInstitute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichInstitute of Medical Virology, University of ZurichDivision of Immunology, University Children’s Hospital ZurichInstitute of Medical Virology, University of ZurichAbstract Background We report a rare case of Mammalian orthoreovirus (MRV) infection in a child with a primary immunodeficiency (PID). Infections with Mammalian orthoreovirus are very rare and probably of zoonotic origin. Only a few cases have been described so far, including one with similar pathogenesis as in our case. Case presentation The patient, age 11, presented with flu-like symptoms and persistent severe diarrhea. Enterovirus has been detected over several months, however, exact typing of a positive cell culture remained inconclusive. Unbiased metagenomic sequencing then detected MRV in stool samples from several time points. The sequencing approach further revealed co-infection with a recombinant Coxsackievirus and Adenovirus. MRV-specific antibodies detected by immunofluorescence proved that the patient seroconverted. Conclusion This case highlights the potential of unbiased metagenomic sequencing in supplementing routine diagnostic methods, especially in situations of chronic infection with multiple viruses as seen here in an immunocompromised host. The origin, transmission routes and implications of MRV infection in humans merit further investigation.http://link.springer.com/article/10.1186/s12879-018-2946-7OrthoreovirusCoxsackievirusAdenovirusPrimary immunodeficiencyMetagenomic sequencing |
| spellingShingle | Dagmara W. Lewandowska Riccarda Capaul Seraina Prader Osvaldo Zagordi Fabienne-Desirée Geissberger Martin Kügler Marcus Knorr Christoph Berger Tayfun Güngör Janine Reichenbach Cyril Shah Jürg Böni Andrea Zbinden Alexandra Trkola Jana Pachlopnik Schmid Michael Huber Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report BMC Infectious Diseases Orthoreovirus Coxsackievirus Adenovirus Primary immunodeficiency Metagenomic sequencing |
| title | Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report |
| title_full | Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report |
| title_fullStr | Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report |
| title_full_unstemmed | Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report |
| title_short | Persistent mammalian orthoreovirus, coxsackievirus and adenovirus co-infection in a child with a primary immunodeficiency detected by metagenomic sequencing: a case report |
| title_sort | persistent mammalian orthoreovirus coxsackievirus and adenovirus co infection in a child with a primary immunodeficiency detected by metagenomic sequencing a case report |
| topic | Orthoreovirus Coxsackievirus Adenovirus Primary immunodeficiency Metagenomic sequencing |
| url | http://link.springer.com/article/10.1186/s12879-018-2946-7 |
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