Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis
IntroductionStaphylococcus epidermidis infections are an important concern in worldwide, especially when associated with biofilms, and resistance of this agent to many drugs makes the situation even worse. We investigated the inhibitory effect of Disaspidin BB obtained from plant extracts and purifi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.999449/full |
| _version_ | 1797948126240178176 |
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| author | Shihua Lan Xiaofeng Chen Chuanping Yin Shengjun Xie Shuaishuai Wang Rongrong Deng Zhibin Shen Zhibin Shen Zhibin Shen |
| author_facet | Shihua Lan Xiaofeng Chen Chuanping Yin Shengjun Xie Shuaishuai Wang Rongrong Deng Zhibin Shen Zhibin Shen Zhibin Shen |
| author_sort | Shihua Lan |
| collection | DOAJ |
| description | IntroductionStaphylococcus epidermidis infections are an important concern in worldwide, especially when associated with biofilms, and resistance of this agent to many drugs makes the situation even worse. We investigated the inhibitory effect of Disaspidin BB obtained from plant extracts and purifications on clinical S. epidermidis strains and their biofilms, and preliminarily investigated its mechanism of of its anti-biofilm activity.Methods and ResultsThe broth dilution method was used to determine the minimum inhibitory concentrations (MIC) of Disaspidin BB on 11 clinical S. epidermidis strains (MIC value of 0.63 ~ 2.5 μg/ml). SEP-05 was found to be erythromycin-resistant (MIC value>8 μg/ml) and Disaspidin BB sensitive with an MIC value of 0.63 μg/ml. The time-kill curve assay indicated that the antibacterial activity of Disaspidin BB against SEP-05 with concentration dependence. The metabolic activity and total biomass of the drug-treated SEP-05 biofilm in each stage were significantly inhibited by the crystalline violet and XTT assay, and the scavenging effect of Disaspidin BB on SEP-05 biofilm was also confirmed by SEM observation. The results of real-time quantitative PCR showed that subinhibitory concentrations Disaspidin BB can inhibit biofilm formation by affecting the expression level of key genes (aap, atlE, icaA, luxS, recA) in SEP-05 biofilm formation. In addition, the content of polysaccharides, proteins and extracellular DNA in biofilm matrix after the intervention of Disaspidin BB was significantly reduced, and it was tentatively determined that the ability of SEP-05 biofilm formation and its stability were thus disturbed.DiscussionThe results show that Disaspidin BB has promising antibacterial effect on erythromycin-resistant S. epidermidis and significant scavenging effect on its biofilm, which provides a theoretical basis for the further development of BB as a new drug for the treatment of skin infections caused by S. epidermidis. |
| first_indexed | 2024-04-10T21:39:39Z |
| format | Article |
| id | doaj.art-cb09d46479794fb6a03c31c821a5d921 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T21:39:39Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cb09d46479794fb6a03c31c821a5d9212023-01-19T07:38:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011410.3389/fmicb.2023.999449999449Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidisShihua Lan0Xiaofeng Chen1Chuanping Yin2Shengjun Xie3Shuaishuai Wang4Rongrong Deng5Zhibin Shen6Zhibin Shen7Zhibin Shen8School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaGuangzhou Hipower Pharmaceutical R&D Co., Ltd., Guangzhou, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaSchool of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, ChinaGuangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou, ChinaGuangdong Cosmetics Engineering and Technology Research Center, Guangdong Pharmaceutical University, Guangzhou, ChinaIntroductionStaphylococcus epidermidis infections are an important concern in worldwide, especially when associated with biofilms, and resistance of this agent to many drugs makes the situation even worse. We investigated the inhibitory effect of Disaspidin BB obtained from plant extracts and purifications on clinical S. epidermidis strains and their biofilms, and preliminarily investigated its mechanism of of its anti-biofilm activity.Methods and ResultsThe broth dilution method was used to determine the minimum inhibitory concentrations (MIC) of Disaspidin BB on 11 clinical S. epidermidis strains (MIC value of 0.63 ~ 2.5 μg/ml). SEP-05 was found to be erythromycin-resistant (MIC value>8 μg/ml) and Disaspidin BB sensitive with an MIC value of 0.63 μg/ml. The time-kill curve assay indicated that the antibacterial activity of Disaspidin BB against SEP-05 with concentration dependence. The metabolic activity and total biomass of the drug-treated SEP-05 biofilm in each stage were significantly inhibited by the crystalline violet and XTT assay, and the scavenging effect of Disaspidin BB on SEP-05 biofilm was also confirmed by SEM observation. The results of real-time quantitative PCR showed that subinhibitory concentrations Disaspidin BB can inhibit biofilm formation by affecting the expression level of key genes (aap, atlE, icaA, luxS, recA) in SEP-05 biofilm formation. In addition, the content of polysaccharides, proteins and extracellular DNA in biofilm matrix after the intervention of Disaspidin BB was significantly reduced, and it was tentatively determined that the ability of SEP-05 biofilm formation and its stability were thus disturbed.DiscussionThe results show that Disaspidin BB has promising antibacterial effect on erythromycin-resistant S. epidermidis and significant scavenging effect on its biofilm, which provides a theoretical basis for the further development of BB as a new drug for the treatment of skin infections caused by S. epidermidis.https://www.frontiersin.org/articles/10.3389/fmicb.2023.999449/fullDisaspidin BBantibacterialStaphylococcus epidermidisanti-biofilm activityantibiotics resistanceanti-biofilm mechanism |
| spellingShingle | Shihua Lan Xiaofeng Chen Chuanping Yin Shengjun Xie Shuaishuai Wang Rongrong Deng Zhibin Shen Zhibin Shen Zhibin Shen Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis Frontiers in Microbiology Disaspidin BB antibacterial Staphylococcus epidermidis anti-biofilm activity antibiotics resistance anti-biofilm mechanism |
| title | Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis |
| title_full | Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis |
| title_fullStr | Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis |
| title_full_unstemmed | Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis |
| title_short | Antibacterial and anti-biofilm activities of Disaspidin BB against Staphylococcus epidermidis |
| title_sort | antibacterial and anti biofilm activities of disaspidin bb against staphylococcus epidermidis |
| topic | Disaspidin BB antibacterial Staphylococcus epidermidis anti-biofilm activity antibiotics resistance anti-biofilm mechanism |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.999449/full |
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