Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations
Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedn...
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| Format: | Article |
| Language: | English |
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Universitas Brawijaya
2018-04-01
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| Series: | AGRIVITA Journal of Agricultural Science |
| Subjects: | |
| Online Access: | http://agrivita.ub.ac.id/index.php/agrivita/article/view/1365 |
| _version_ | 1817997130206281728 |
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| author | Halimaton Saadiah Othman Mohamad Osman Zarina Zainuddin |
| author_facet | Halimaton Saadiah Othman Mohamad Osman Zarina Zainuddin |
| author_sort | Halimaton Saadiah Othman |
| collection | DOAJ |
| description | Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedness among stevia accessions in order to broaden the genetic basis for future stevia breeding. This study described morphological and chemical variations and investigates genetic relationships among stevia accessions derived from across Malaysia and Paraguay using inter simple sequence repeats (ISSR) markers. HPLC (high-performance liquid chromatography) analysis also revealed high variability with stevioside content between 4.54 % (Taman Pertanian) to 20.36 % (Bangi) and rebaudioside A content varied between 0.3 % (Nilai) to 2.04 % (MNQ). From 32 ISSR markers, a total of 332 bands were scored, of which 264 (78 %) were polymorphic. The dendrogram from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis separated 17 stevia accessions into 3 main groups. Rawang and Nilai were found to be closely related. The wide genetic variabilities among stevia accessions are a promising indicator towards the development of new stevia varieties. This valuable information will be able to assist parental selection in future stevia breeding programmes. |
| first_indexed | 2024-04-14T02:32:37Z |
| format | Article |
| id | doaj.art-cb0a35800fda420a8754701622eabb5d |
| institution | Directory Open Access Journal |
| issn | 0126-0537 2477-8516 |
| language | English |
| last_indexed | 2024-04-14T02:32:37Z |
| publishDate | 2018-04-01 |
| publisher | Universitas Brawijaya |
| record_format | Article |
| series | AGRIVITA Journal of Agricultural Science |
| spelling | doaj.art-cb0a35800fda420a8754701622eabb5d2022-12-22T02:17:37ZengUniversitas BrawijayaAGRIVITA Journal of Agricultural Science0126-05372477-85162018-04-0140226728310.17503/agrivita.v40i2.1365398Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular CharacterisationsHalimaton Saadiah Othman0Mohamad Osman1Zarina Zainuddin2International Islamic University Malaysia, MalaysiaUniversiti Putra Malaysia, MalaysiaInternational Islamic University Malaysia, MalaysiaStevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedness among stevia accessions in order to broaden the genetic basis for future stevia breeding. This study described morphological and chemical variations and investigates genetic relationships among stevia accessions derived from across Malaysia and Paraguay using inter simple sequence repeats (ISSR) markers. HPLC (high-performance liquid chromatography) analysis also revealed high variability with stevioside content between 4.54 % (Taman Pertanian) to 20.36 % (Bangi) and rebaudioside A content varied between 0.3 % (Nilai) to 2.04 % (MNQ). From 32 ISSR markers, a total of 332 bands were scored, of which 264 (78 %) were polymorphic. The dendrogram from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis separated 17 stevia accessions into 3 main groups. Rawang and Nilai were found to be closely related. The wide genetic variabilities among stevia accessions are a promising indicator towards the development of new stevia varieties. This valuable information will be able to assist parental selection in future stevia breeding programmes.http://agrivita.ub.ac.id/index.php/agrivita/article/view/1365Genetic VariabilitiesISSR MarkerMorphologyStevia rebaudianaSteviol glycosides |
| spellingShingle | Halimaton Saadiah Othman Mohamad Osman Zarina Zainuddin Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations AGRIVITA Journal of Agricultural Science Genetic Variabilities ISSR Marker Morphology Stevia rebaudiana Steviol glycosides |
| title | Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations |
| title_full | Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations |
| title_fullStr | Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations |
| title_full_unstemmed | Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations |
| title_short | Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations |
| title_sort | genetic variabilities of stevia rebaudiana bertoni cultivated in malaysia as revealed by morphological chemical and molecular characterisations |
| topic | Genetic Variabilities ISSR Marker Morphology Stevia rebaudiana Steviol glycosides |
| url | http://agrivita.ub.ac.id/index.php/agrivita/article/view/1365 |
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