A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography
Ten compounds, including three lignan glycosides and seven lignans, were purified from Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H2O (1:0.7:1:0.7, v/v...
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MDPI AG
2017-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/12/2024 |
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| author | Jiaojiao Jiang Hongjing Dong Tao Wang Ruixuan Zhao Yan Mu Yanling Geng Zhenjia Zheng Xiao Wang |
| author_facet | Jiaojiao Jiang Hongjing Dong Tao Wang Ruixuan Zhao Yan Mu Yanling Geng Zhenjia Zheng Xiao Wang |
| author_sort | Jiaojiao Jiang |
| collection | DOAJ |
| description | Ten compounds, including three lignan glycosides and seven lignans, were purified from Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H2O (1:0.7:1:0.7, v/v) was firstly employed to separate the crude extract (320 mg), from which 19.3 mg of justicidin B (f), 10.8 mg of justicidin A (g), 13.9 mg of 6′-hydroxyjusticidin C (h), 7.7 mg of justicidin E (i), 6.3 mg of lignan J1 (j) were obtained with 91.3 mg of enriched mixture of compounds a–e. The enriched mixture (91.3 mg) was further separated using the solvent system consisting of petroleum–ethyl acetate–methanol–H2O (3:3.8:3:3.8, v/v), yielding 12.1 mg of procumbenoside E (a); 7.6 mg of diphyllin-1-O-β-d-apiofuranoside (b); 7.4 mg of diphyllin (c); 8.3 mg of 6′-hydroxy justicidin B (d); and 7.9 mg of diphyllin acetyl apioside (e). The purities of the 10 components were all above 94%, and their structures were identified by NMR and ESI-MS spectra. The results demonstrated that the strategy based on HSCCC for the separation of lignans and their glycosides was efficient and rapid. |
| first_indexed | 2024-12-11T18:12:58Z |
| format | Article |
| id | doaj.art-cb0aa04d6fd04a11a28b4e8e257d618e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T18:12:58Z |
| publishDate | 2017-11-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cb0aa04d6fd04a11a28b4e8e257d618e2022-12-22T00:55:31ZengMDPI AGMolecules1420-30492017-11-012212202410.3390/molecules22122024molecules22122024A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current ChromatographyJiaojiao Jiang0Hongjing Dong1Tao Wang2Ruixuan Zhao3Yan Mu4Yanling Geng5Zhenjia Zheng6Xiao Wang7Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaKey Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaKey Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaKey Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Taian 271018, ChinaKey Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaTen compounds, including three lignan glycosides and seven lignans, were purified from Justicia procumbens L. in 8 h using an efficient strategy based on high-speed counter-current chromatography (HSCCC). The two-phase solvent system composed of petroleum–ethyl acetate–methanol–H2O (1:0.7:1:0.7, v/v) was firstly employed to separate the crude extract (320 mg), from which 19.3 mg of justicidin B (f), 10.8 mg of justicidin A (g), 13.9 mg of 6′-hydroxyjusticidin C (h), 7.7 mg of justicidin E (i), 6.3 mg of lignan J1 (j) were obtained with 91.3 mg of enriched mixture of compounds a–e. The enriched mixture (91.3 mg) was further separated using the solvent system consisting of petroleum–ethyl acetate–methanol–H2O (3:3.8:3:3.8, v/v), yielding 12.1 mg of procumbenoside E (a); 7.6 mg of diphyllin-1-O-β-d-apiofuranoside (b); 7.4 mg of diphyllin (c); 8.3 mg of 6′-hydroxy justicidin B (d); and 7.9 mg of diphyllin acetyl apioside (e). The purities of the 10 components were all above 94%, and their structures were identified by NMR and ESI-MS spectra. The results demonstrated that the strategy based on HSCCC for the separation of lignans and their glycosides was efficient and rapid.https://www.mdpi.com/1420-3049/22/12/2024Justicia procumbens L.lignans and their glycosidespreparative separationhigh-speed counter-current chromatography |
| spellingShingle | Jiaojiao Jiang Hongjing Dong Tao Wang Ruixuan Zhao Yan Mu Yanling Geng Zhenjia Zheng Xiao Wang A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography Molecules Justicia procumbens L. lignans and their glycosides preparative separation high-speed counter-current chromatography |
| title | A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography |
| title_full | A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography |
| title_fullStr | A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography |
| title_full_unstemmed | A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography |
| title_short | A Strategy for Preparative Separation of 10 Lignans from Justicia procumbens L. by High-Speed Counter-Current Chromatography |
| title_sort | strategy for preparative separation of 10 lignans from justicia procumbens l by high speed counter current chromatography |
| topic | Justicia procumbens L. lignans and their glycosides preparative separation high-speed counter-current chromatography |
| url | https://www.mdpi.com/1420-3049/22/12/2024 |
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