The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2021-10-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4034 |
| _version_ | 1797394299803926528 |
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| author | Danuta Kozak Marzena Parzymies Alicja Świstowska Barbara Marcinek Elżbieta Pogroszewska |
| author_facet | Danuta Kozak Marzena Parzymies Alicja Świstowska Barbara Marcinek Elżbieta Pogroszewska |
| author_sort | Danuta Kozak |
| collection | DOAJ |
| description |
Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media and explants orientation on multiplication of Mandevilla sanderi, an exotic, ornamental pot plant. The shoot tips were placed vertically or horizontally on the Murashige and Skoog medium supplemented with benzyladenine (BA) or isopentenyladenine (2iP), at concentrations of 1, 2.5 or 5 mg·dm–3 singly or in combination with thidiazuron (TDZ) at concentrations of 0.01, 0.025 or 0.05 mg·dm–3. Maximum multiplication rate was noted on the media supplemented with 2.5 mg·dm–3 2iP + 0.025 mg·dm–3 TDZ or 5 mg·dm–3 2iP, when explants were placed horizontally. All the treatments resulted in callus formation. Medium supplemented with the highest concentration of BA combined with TDZ was the most active in callus growth.
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| first_indexed | 2024-03-09T00:17:37Z |
| format | Article |
| id | doaj.art-cb0ca7c50b914a5aa953db88a1b701a3 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:17:37Z |
| publishDate | 2021-10-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-cb0ca7c50b914a5aa953db88a1b701a32023-12-12T07:49:15ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052021-10-0120510.24326/2021.5.12The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitroDanuta Kozak0Marzena Parzymies1Alicja Świstowska2Barbara Marcinek3Elżbieta Pogroszewska4University of Life Sciences in LublinUniversity of Life Sciences in Lublin, Lublin, PolandUniversity of Life Sciences in LublinUniversity of Life Sciences in LublinUniversity of Life Sciences in Lublin Mandevilla is a valuable ornamental pot vine. However, due to a low propagation rate, it is difficult to keep up with the demand. Micropropagation would allow to produce lots of plants for the market. The aim of the study was to determine the effect of the growth regulators addition to the media and explants orientation on multiplication of Mandevilla sanderi, an exotic, ornamental pot plant. The shoot tips were placed vertically or horizontally on the Murashige and Skoog medium supplemented with benzyladenine (BA) or isopentenyladenine (2iP), at concentrations of 1, 2.5 or 5 mg·dm–3 singly or in combination with thidiazuron (TDZ) at concentrations of 0.01, 0.025 or 0.05 mg·dm–3. Maximum multiplication rate was noted on the media supplemented with 2.5 mg·dm–3 2iP + 0.025 mg·dm–3 TDZ or 5 mg·dm–3 2iP, when explants were placed horizontally. All the treatments resulted in callus formation. Medium supplemented with the highest concentration of BA combined with TDZ was the most active in callus growth. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4034DipladeniaRocktrumpetmicropropagationcytokininsexplant orientation |
| spellingShingle | Danuta Kozak Marzena Parzymies Alicja Świstowska Barbara Marcinek Elżbieta Pogroszewska The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro Acta Scientiarum Polonorum: Hortorum Cultus Dipladenia Rocktrumpet micropropagation cytokinins explant orientation |
| title | The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro |
| title_full | The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro |
| title_fullStr | The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro |
| title_full_unstemmed | The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro |
| title_short | The influence of growth regulators and explant position on the growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro |
| title_sort | influence of growth regulators and explant position on the growth and development of mandevilla sanderi hemsl woodson in vitro |
| topic | Dipladenia Rocktrumpet micropropagation cytokinins explant orientation |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/4034 |
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