Sweat lipid mediator profiling: a noninvasive approach for cutaneous research
Recent advances in analytical and sweat collection techniques provide new opportunities to identify noninvasive biomarkers for the study of skin inflammation and repair. This study aims to characterize the lipid mediator profile including oxygenated lipids, endocannabinoids, and ceramides/sphingoid...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520314462 |
| _version_ | 1818410024906522624 |
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| author | Karan Agrawal Lauren A. Hassoun Negar Foolad Theresa L. Pedersen Raja K. Sivamani John W. Newman |
| author_facet | Karan Agrawal Lauren A. Hassoun Negar Foolad Theresa L. Pedersen Raja K. Sivamani John W. Newman |
| author_sort | Karan Agrawal |
| collection | DOAJ |
| description | Recent advances in analytical and sweat collection techniques provide new opportunities to identify noninvasive biomarkers for the study of skin inflammation and repair. This study aims to characterize the lipid mediator profile including oxygenated lipids, endocannabinoids, and ceramides/sphingoid bases in sweat and identify differences in these profiles between sweat collected from nonlesional sites on the unflared volar forearm of subjects with and without atopic dermatitis (AD). Adapting routine procedures developed for plasma analysis, over 100 lipid mediators were profiled using LC-MS/MS and 58 lipid mediators were detected in sweat. Lipid mediator concentrations were not affected by sampling or storage conditions. Increases in concentrations of C30–C40 [NS] and [NdS] ceramides, and C18:1 sphingosine, were observed in the sweat of study participants with AD despite no differences being observed in transepidermal water loss between study groups, and this effect was strongest in men (P < 0.05, one-way ANOVA with Tukey's post hoc HSD). No differences in oxylipins and endocannabinoids were observed between study groups. Sweat mediator profiling may therefore provide a noninvasive diagnostic for AD prior to the presentation of clinical signs. |
| first_indexed | 2024-12-14T10:08:57Z |
| format | Article |
| id | doaj.art-cb0d4fc4200c464ab9bef1317761fa88 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T10:08:57Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-cb0d4fc4200c464ab9bef1317761fa882022-12-21T23:07:04ZengElsevierJournal of Lipid Research0022-22752017-01-01581188195Sweat lipid mediator profiling: a noninvasive approach for cutaneous researchKaran Agrawal0Lauren A. Hassoun1Negar Foolad2Theresa L. Pedersen3Raja K. Sivamani4John W. Newman5Department of Nutrition, University of California-Davis, Davis, CA 95616; National Institutes of Health West Coast Metabolomics Center, Davis, CA 95616Department of Dermatology, University of California-Davis Medical Center, Sacramento, CA 95816Department of Dermatology, University of California-Davis Medical Center, Sacramento, CA 95816National Institutes of Health West Coast Metabolomics Center, Davis, CA 95616; United States Department of Agriculture, Agricultural Research Service, Western Human Nutrition Research Center, Davis, CA 95616Department of Dermatology, University of California-Davis Medical Center, Sacramento, CA 95816Department of Nutrition, University of California-Davis, Davis, CA 95616; National Institutes of Health West Coast Metabolomics Center, Davis, CA 95616; United States Department of Agriculture, Agricultural Research Service, Western Human Nutrition Research Center, Davis, CA 95616; To whom correspondence should be addressed.Recent advances in analytical and sweat collection techniques provide new opportunities to identify noninvasive biomarkers for the study of skin inflammation and repair. This study aims to characterize the lipid mediator profile including oxygenated lipids, endocannabinoids, and ceramides/sphingoid bases in sweat and identify differences in these profiles between sweat collected from nonlesional sites on the unflared volar forearm of subjects with and without atopic dermatitis (AD). Adapting routine procedures developed for plasma analysis, over 100 lipid mediators were profiled using LC-MS/MS and 58 lipid mediators were detected in sweat. Lipid mediator concentrations were not affected by sampling or storage conditions. Increases in concentrations of C30–C40 [NS] and [NdS] ceramides, and C18:1 sphingosine, were observed in the sweat of study participants with AD despite no differences being observed in transepidermal water loss between study groups, and this effect was strongest in men (P < 0.05, one-way ANOVA with Tukey's post hoc HSD). No differences in oxylipins and endocannabinoids were observed between study groups. Sweat mediator profiling may therefore provide a noninvasive diagnostic for AD prior to the presentation of clinical signs.http://www.sciencedirect.com/science/article/pii/S0022227520314462oxidized lipidsendocannabinoidsceramidessphingolipidsskinmetabolic profiling |
| spellingShingle | Karan Agrawal Lauren A. Hassoun Negar Foolad Theresa L. Pedersen Raja K. Sivamani John W. Newman Sweat lipid mediator profiling: a noninvasive approach for cutaneous research Journal of Lipid Research oxidized lipids endocannabinoids ceramides sphingolipids skin metabolic profiling |
| title | Sweat lipid mediator profiling: a noninvasive approach for cutaneous research |
| title_full | Sweat lipid mediator profiling: a noninvasive approach for cutaneous research |
| title_fullStr | Sweat lipid mediator profiling: a noninvasive approach for cutaneous research |
| title_full_unstemmed | Sweat lipid mediator profiling: a noninvasive approach for cutaneous research |
| title_short | Sweat lipid mediator profiling: a noninvasive approach for cutaneous research |
| title_sort | sweat lipid mediator profiling a noninvasive approach for cutaneous research |
| topic | oxidized lipids endocannabinoids ceramides sphingolipids skin metabolic profiling |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520314462 |
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