Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?

Joint inflammatory diseases are debilitating and very painful conditions that still lack effective treatments. Recently, glial cells were shown to be crucial for the development and maintenance of chronic pain, constituting novel targets for therapeutic approaches. At the periphery, the satellite gl...

Full description

Bibliographic Details
Main Authors: Diana Sofia Marques Nascimento, José Manuel Castro-Lopes, Fani Lourença Moreira Neto
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4172763?pdf=render
_version_ 1830358067018989568
author Diana Sofia Marques Nascimento
José Manuel Castro-Lopes
Fani Lourença Moreira Neto
author_facet Diana Sofia Marques Nascimento
José Manuel Castro-Lopes
Fani Lourença Moreira Neto
author_sort Diana Sofia Marques Nascimento
collection DOAJ
description Joint inflammatory diseases are debilitating and very painful conditions that still lack effective treatments. Recently, glial cells were shown to be crucial for the development and maintenance of chronic pain, constituting novel targets for therapeutic approaches. At the periphery, the satellite glial cells (SGCs) that surround the cell bodies of primary afferents neurons in the dorsal root ganglia (DRG) display hypertrophy, proliferation, and activation following injury and/or inflammation. It has been suggested that the expression of neuronal injury factors might initially trigger these SGCs-related events. We then aimed at evaluating if SGCs are involved in the establishment/maintenance of articular inflammatory pain, by using the monoarthritis (MA) model, and if the neuronal injury marker activating transcriptional factor 3 (ATF3) is associated with these SGCs' reactive changes. Western Blot (WB) analysis of the glial fibrillary acidic protein (GFAP) expression was performed in L4-L5 DRGs from control non-inflamed rats and MA animals at different time-points of disease (4, 7, and 14d, induced by complete Freund's adjuvant injection into the left hind paw ankle joint). Data indicate that SGCs activation is occurring in MA animals, particularly after day 7 of disease evolution. Additionally, double-immunostaining for ATF3 and GFAP in L5 DRG sections shows that SGCs's activation significantly increases around stressed neurons at 7d of disease, when compared with control animals. The specific labelling of GFAP in SGCs rather than in other cell types was also confirmed by immunohistochemical labeling. Finally, BrdU incorporation indicates that proliferation of SGCs is also significantly increased after 7 days of MA. Data indicate that SGCs play an important role in the mechanisms of articular inflammation, with 7 days of disease being a critical time-point in the MA model, and suggest that ATF3 might be involved in SGCs' reactive changes such as activation.
first_indexed 2024-12-20T02:34:36Z
format Article
id doaj.art-cb115783ad9f49a194b80bf45a029aa2
institution Directory Open Access Journal
issn 1932-6203
language English
last_indexed 2024-12-20T02:34:36Z
publishDate 2014-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj.art-cb115783ad9f49a194b80bf45a029aa22022-12-21T19:56:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10815210.1371/journal.pone.0108152Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?Diana Sofia Marques NascimentoJosé Manuel Castro-LopesFani Lourença Moreira NetoJoint inflammatory diseases are debilitating and very painful conditions that still lack effective treatments. Recently, glial cells were shown to be crucial for the development and maintenance of chronic pain, constituting novel targets for therapeutic approaches. At the periphery, the satellite glial cells (SGCs) that surround the cell bodies of primary afferents neurons in the dorsal root ganglia (DRG) display hypertrophy, proliferation, and activation following injury and/or inflammation. It has been suggested that the expression of neuronal injury factors might initially trigger these SGCs-related events. We then aimed at evaluating if SGCs are involved in the establishment/maintenance of articular inflammatory pain, by using the monoarthritis (MA) model, and if the neuronal injury marker activating transcriptional factor 3 (ATF3) is associated with these SGCs' reactive changes. Western Blot (WB) analysis of the glial fibrillary acidic protein (GFAP) expression was performed in L4-L5 DRGs from control non-inflamed rats and MA animals at different time-points of disease (4, 7, and 14d, induced by complete Freund's adjuvant injection into the left hind paw ankle joint). Data indicate that SGCs activation is occurring in MA animals, particularly after day 7 of disease evolution. Additionally, double-immunostaining for ATF3 and GFAP in L5 DRG sections shows that SGCs's activation significantly increases around stressed neurons at 7d of disease, when compared with control animals. The specific labelling of GFAP in SGCs rather than in other cell types was also confirmed by immunohistochemical labeling. Finally, BrdU incorporation indicates that proliferation of SGCs is also significantly increased after 7 days of MA. Data indicate that SGCs play an important role in the mechanisms of articular inflammation, with 7 days of disease being a critical time-point in the MA model, and suggest that ATF3 might be involved in SGCs' reactive changes such as activation.http://europepmc.org/articles/PMC4172763?pdf=render
spellingShingle Diana Sofia Marques Nascimento
José Manuel Castro-Lopes
Fani Lourença Moreira Neto
Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?
PLoS ONE
title Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?
title_full Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?
title_fullStr Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?
title_full_unstemmed Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?
title_short Satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats: is there a role for ATF3?
title_sort satellite glial cells surrounding primary afferent neurons are activated and proliferate during monoarthritis in rats is there a role for atf3
url http://europepmc.org/articles/PMC4172763?pdf=render
work_keys_str_mv AT dianasofiamarquesnascimento satelliteglialcellssurroundingprimaryafferentneuronsareactivatedandproliferateduringmonoarthritisinratsistherearoleforatf3
AT josemanuelcastrolopes satelliteglialcellssurroundingprimaryafferentneuronsareactivatedandproliferateduringmonoarthritisinratsistherearoleforatf3
AT fanilourencamoreiraneto satelliteglialcellssurroundingprimaryafferentneuronsareactivatedandproliferateduringmonoarthritisinratsistherearoleforatf3